Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Human Pachytene checkpoint protein 2 homolog (TRIP13)

Recombinant Human Pachytene checkpoint protein 2 homolog (TRIP13)

  • 货号：
    CSB-YP623008HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP623008HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP623008HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP623008HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TRIP13
  • Uniprot No.：
  • 别名：
    16E1-BP; 16E1BP; Homo sapiens HPV16 E1 protein binding protein mRNA complete cds; HPV16 E1 protein binding protein; HPV16 E1 protein-binding protein; Human papillomavirus type 16 E1 protein binding protein; Human papillomavirus type 16 E1 protein-binding protein; Pachytene checkpoint protein 2 homolog; PCH2; Thyroid hormone receptor interactor 13; Thyroid receptor interacting protein 13; Thyroid receptor-interacting protein 13; TR-interacting protein 13; TRIP-13; Trip13; TRP13_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-432
  • 氨基酸序列
    MDEAVGDLKQ ALPCVAESPT VHVEVHQRGS STAKKEDINL SVRKLLNRHN IVFGDYTWTE FDEPFLTRNV QSVSIIDTEL KVKDSQPIDL SACTVALHIF QLNEDGPSSE NLEEETENII AANHWVLPAA EFHGLWDSLV YDVEVKSHLL DYVMTTLLFS DKNVNSNLIT WNRVVLLHGP PGTGKTSLCK ALAQKLTIRL SSRYRYGQLI EINSHSLFSK WFSESGKLVT KMFQKIQDLI DDKDALVFVL IDEVESLTAA RNACRAGTEP SDAIRVVNAV LTQIDQIKRH SNVVILTTSN ITEKIDVAFV DRADIKQYIG PPSAAAIFKI YLSCLEELMK CQIIYPRQQL LTLRELEMIG FIENNVSKLS LLLNDISRKS EGLSGRVLRK LPFLAHALYV QAPTVTIEGF LQALSLAVDK QFEERKKLAA YI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a key role in chromosome recombination and chromosome structure development during meiosis. Required at early steps in meiotic recombination that leads to non-crossovers pathways. Also needed for efficient completion of homologous synapsis by influencing crossover distribution along the chromosomes affecting both crossovers and non-crossovers pathways. Also required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation. In males, required for efficient synapsis of the sex chromosomes and for sex body formation. Promotes early steps of the DNA double-strand breaks (DSBs) repair process upstream of the assembly of RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from synapsed chromosomes. Plays a role in mitotic spindle assembly checkpoint (SAC) activation.
  • 基因功能参考文献：
    1. heightened TRIP13 expression appears to promote lung adenocarcinoma tumor progression. PMID: 29567476
    2. TRIP13-p31(comet) intercepts and disassembles free mitotic checkpoint complex not bound to anaphase-promoting complex/cyclosome through mediating the local unfolding of the Mad2 C-terminal region. PMID: 29208896
    3. Microarray analyses indicated that the biological function of TRIP13 in CLL is majorly cell apoptosis and cell proliferation associated. TRIP13 siRNA expressing cells exhibited a slower cell proliferation rate and underwent apoptosis compared with control cells. PMID: 28424416
    4. TRIP13 ubiquitinates and degrades the checkpoint surveillance protein MAD2 via activating Akt signaling pathway, leading to weakened checkpoint surveillance and consequent tumorigenic aneuploidy and drug resistance in MM. PMID: 28157697
    5. These results identify an unexpected dependency on TRIP13 in cells overexpressing Mad2. PMID: 28564602
    6. 6 individuals with biallelic loss-of-function mutations in TRIP13 developed Wilms tumors. TRIP13-mutant patient cells have no detectable TRIP13 and have substantial impairment of the spindle assembly checkpoint (SAC), leading to a high rate of chromosome missegregation. Individuals with biallelic TRIP13 mutations have a high risk of embryonal tumors. These experiments show that their cells display severe SAC impairment. PMID: 28553959
    7. Thes s show that p31(comet) binding to the TRIP13 N-terminal domain positions the disordered MAD2 N-terminus for engagement by the TRIP13 "pore loops", which then unfold MAD2 in the presence of ATP. PMID: 28659378
    8. These results establish a paradigm of the roles of p31(comet) and TRIP13 in both checkpoint activation and inactivation. PMID: 26832417
    9. the oligomeric form of TRIP13 binds both p31(comet) and MCC PMID: 26324890
    10. TRIP13 promotes error-prone non-homologous end joining and induces chemoresistance in head and neck cancer. PMID: 25078033
    11. suggest that premature mitotic checkpoint silencing triggered by TRIP13 overexpression may promote cancer development PMID: 25012665
    12. propose that TRIP13 plays centrally important roles in the sequence of events leading to MCC disassembly and checkpoint inactivation PMID: 25092294

    显示更多

    收起更多

  • 相关疾病：
    Mosaic variegated aneuploidy syndrome 3 (MVA3)
  • 蛋白家族：
    AAA ATPase family, PCH2 subfamily
  • 数据库链接：

    HGNC: 12307

    OMIM: 604507

    KEGG: hsa:9319

    STRING: 9606.ENSP00000166345

    UniGene: Hs.436187