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  4. Recombinant Human PCNA-associated factor (PCLAF)

Recombinant Human PCNA-associated factor (PCLAF)

In Stock
  • 货号：
    CSB-EP012164HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    HCV NS5A transactivated protein 9 ; HCV NS5A-transactivated protein 9; Hepatitis C virus NS5A transactivated protein 9; Hepatitis C virus NS5A-transactivated protein 9; KIAA0101; L5; NS5 ATP9; NS5ATP9; OEATC 1; OEATC; OEATC-1; OEATC1; Overexpressed in anaplastic thyroid carcinoma 1; p15(PAF); p15PAF; PAF; PAF_HUMAN; PCNA associated factor; PCNA-associated factor
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    39.0kDa
  • 表达区域：
    1-111aa
  • 氨基酸序列
    MVRTKADSVPGTYRKVVAARAPRKVLGSSTSATNSTSVSSRKAENKYAGGNPVCVRPTPKWQKGIGEFFRLSPKDSEKENQIPEEAGSSGLGKAKRKACPLQPDHTNDEKE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number.
  • 基因功能参考文献：
    1. These results suggested that KIAA0101 knockdown suppressed the cell proliferation and cell cycle progression by promoting the formation of p53/Sp1 complex in breast cancer. PMID: 29902451
    2. PAF supports glioma stem cells maintenance, in part, by influencing DNA replication and pyrimidine metabolism pathways.PAF-associated DNA translesion synthesis activity influences glioma stem cell radiation resistance. PMID: 29073105
    3. KIAA0101 tv2 exerts anti-tumor activity in HCC and acts as an endogenous competitor of tumor-associated KIAA0101 tv1. KIAA0101 tv2 has a potential to work as a therapeutic drug targeting the KIAA0101 tv1 in HCC. PMID: 28410205
    4. Paf15 expression is associated with increased rectal cancer proliferation, decreased patient survival, and a worse radiotherapeutic response PMID: 27246972
    5. High KIAA0101 expression is associated with kidney cancer. PMID: 26575329
    6. The complex between p15PAF and trimeric PCNA is of low affinity, forming a transient complex that is difficult to characterize at a structural level due to its inherent polydispersity. We have determined the structure, conformational fluctuations, and relative population of the five species that coexist in solution by combining small-angle X-ray scattering (SAXS) with molecular modelling. PMID: 28180305
    7. miR-429 mediates deregulation of KIAA0101 by acting as an anti-metastatic miRNA that targets KIAA0101 pro-metastatic gene during metastasis of soft tissue sarcomas PMID: 28432002
    8. PAF stimulation dose-dependently promoted the invasion, migration and growth of prostate cancer cells in vitro, while knockdow our findings demonstrate that PAFR can activate ERK1/2 pathway, and subsequently increase MMP-3 expression and decrease E-cadherin expression PMID: 27176648
    9. High KIAA0101 level is associated with metastasis in hepatocellular carcinoma. PMID: 26968109
    10. Results unveil an unsuspected role of the PAF-Wnt signalling axis in modulating cell plasticity, which is required for the maintenance of breast cancer cell stemness. PMID: 26843124
    11. IN PATIENTS WITH HEPATITIS C VIRUS GENOTYPE 1B, TREATMENT RESPONSE WITH DACLATASVIR PLUS ASUNAPREVIR WITHOUT NS5A-L31F/I/M/V and/or NS5A-Y93H polymorphisms PMID: 26155891
    12. p15PAF acts as a flexible drag that regulates PCNA sliding along the DNA and facilitates the switch from replicative to translesion synthesis polymerase binding. PMID: 25762514
    13. Data show that hepatitis C virus NS5A-transactivated protein 9 (NS5ATP9) knockdown activated mitogen-activated protein kinase kinase/extracellular signal regulated kinases 1/2 signaling under C virus nonstructural protein 5A (NS5A) expression. PMID: 23698777
    14. NS5ATP9 mRNA overexpression in peripheral blood mononuclear cells was significantly associated with poor disease-free survival and overall survival of gastric cancer patients, but not with tumor recurrence. PMID: 24996800
    15. These results highlight an important potential role for NS5ATP9 in hepatitis C virus NS5A-induced hepatocyte autophagy. PMID: 24750205
    16. overexpression of KIAA0101 mRNA in peripheral blood mononuclear cells could act as a predictive biomarker for hepatic cancer PMID: 24197986
    17. Upregulated expression of KIAA0101 is associated with esophageal cancer progression, resistance to chemotherapy, and poor survival. PMID: 24145239
    18. Data indicate that PAF-EZH2-beta-catenin complex hyperactivates Wnt signaling. PMID: 24055345
    19. p15(PAF) is tightly regulated by the Rb/E2F complex. Loss of Rb/E2F-mediated repression during the G1/S transition phase leads to p15(PAF) upregulation, which facilitates DNA synthesis and S-phase progression. PMID: 23593430
    20. Low expression of PAF and high expression of TNF-alpha in leukocytospermia affect the sperm motility, which is one of the reasons that leads to infertility. PMID: 23209346
    21. GC patients with elevated KIAA0101 expression levels exhibited a high recurrence and subsequently poor prognosis in the survival study PMID: 23240630
    22. Down-regulation of KIAA0101 expression leads to an inhibition of cell proliferation, cell cycle and cell invasion of gastric carcinoma. PMID: 23157749
    23. KIAA0101 transcript variant 1 may function as a regulator, promoting cell survival in hepatocellular carcinoma through regulating the function of p53. PMID: 22576474
    24. The results uncovered a critical role for PCNA-associated factor PAF15 (p15(PAF)/KIAA0101) ubiquitylation during DNA replication. During unperturbed S phase, chromatin-associated PAF15 is modified by double mono-ubiquitylation of lysine 15 and 24. PMID: 23000965
    25. High-level KIAA0101 expression was observed in 33.9% (121 of 357 cases). PMID: 21689861
    26. Data supports that KIAA0101 is a marker of cellular proliferation, promotes growth and invasion, and is a good diagnostic marker for distinguishing benign from malignant adrenocortical neoplasm. PMID: 22096502
    27. This study identifies KIAA0101 as a protein important for breast tumorigenesis, and as this factor has been reported as a UV repair factor, it may link the UV damage response to centrosome control PMID: 21673012
    28. Data indicate that PAF15 associates with PCNA, and depletion of PAF15 decreases the number of cells in S phase, suggesting a role for it in cell cycle regulation. PMID: 21628590
    29. KIAA0101 gene may participate in cell cycle regulation of non-small cell lung cancer. PMID: 20118010
    30. Overexpression of KIAA0101 is associated with anaplastic thyroid carcinoma PMID: 15789362
    31. KIAA0101 protein expression was down-regulated in hepatocellular carcinoma; this gene could inhibit the HCC cell growth in vitro and presumably by its blocking effect on cell cycle PMID: 16646990
    32. NS5ATP9 is a NS5A up-regulation gene which may play a role in the pathogenesis of HCV-associated hepatocellular carcinoma. PMID: 18068894
    33. p15(PAF) gene is regulated by NF-kappa B signal transduction. PMID: 18727915
    34. Interaction between p15(PAF)and Proliferating Cell Nuclear Antigen is required for the DNA repair process. PMID: 19219066
    35. The PCNA-associated factor KIAA0101/p15(PAF) binds the potential tumor suppressor product p33ING1b. PMID: 16288740

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  • 亚细胞定位：
    Nucleus. Cytoplasm, perinuclear region. Note=Following DNA damage, localizes to DNA damage sites (PubMed:21628590). Colocalizes with centrosomes in perinuclear region (PubMed:21673012).
  • 组织特异性：
    Expressed predominantly in liver, pancreas and placenta. Not detected in heart or brain. Highly expressed in a number of tumors, especially esophageal tumors, in anaplastic thyroid carcinomas, adrenocortical carcinomas, and in non-small-cell lung cancer l
  • 数据库链接：

    HGNC: 28961

    OMIM: 610696

    KEGG: hsa:9768

    STRING: 9606.ENSP00000300035

    UniGene: Hs.81892