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  4. Recombinant Human Oxysterols receptor LXR-alpha (NR1H3)

Recombinant Human Oxysterols receptor LXR-alpha (NR1H3)

In Stock
  • 货号：
    CSB-EP619753HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Liver X receptor alpha; LXR a; LXRA; NR1H3; NR1H3_HUMAN; Nuclear receptor subfamily 1 group H member 3; Oxysterols receptor LXR alpha; Oxysterols receptor LXR-alpha; RLD 1; RLD1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Isoform 3
  • 来源：
    E.coli
  • 分子量：
    61.7kDa
  • 表达区域：
    1-402a
  • 氨基酸序列
    MPHSAGGTAGVGLEAAEPTALLTRAEPPSEPTEIRPQKRKKGPAPKMLGNELCSVCGDKASGFHYNVLSCEGCKGFFRRSVIKGAHYICHSGGHCPMDTYMRRKCQECRLRKCRQAGMREECVLSEEQIRLKKLKRQEEEQAHATSLPPRASSPPQILPQLSPEQLGMIEKLVAAQQQCNRRSFSDRLRVTPWPMAPDPHSREARQQRFAHFTELAIVSVQEIVDFAKQLPGFLQLSREDQIALLKTSAIEVMLLETSRRYNPGSESITFLKDFSYNREDFAKAGLQVEFINPIFEFSRAMNELQLNDAEFALLIAISIFSADRPNVQDQLQVERLQHTYVEALHAYVSIHHPHDRLMFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis. Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators.
  • 基因功能参考文献：
    1. The s report that, in Mycobacterium tuberculosis-infected macrophages, IL-36 signaling modulates cholesterol biosynthesis and efflux via LXR. PMID: 29367626
    2. LXR-alpha played a central role in down-regulating of ABCA1 and ABCG1 and lipid accumulation induced by homocysteine in the macrophages. PMID: 30393020
    3. LXRalpha interacts with OGT in its N-terminal domain and ligand-binding domain (LBD) in a ligand-independent fashion. PMID: 29577901
    4. Our data suggest that LXR could be used as a biomarker for hepatocellular carcinoma prognosis. PMID: 28508927
    5. PBMCs from healthy persons were predisposed to the MPhi2 differentiation phenotype, which exhibits elevated cholesterol uptake and anti-inflammatory properties. LXRalpha over-expression polarizes macrophages towards the anti-inflammatory MPhi2 phenotype. PMID: 29420090
    6. LXR gene expression was significantly increased in obese children with obstructive sleep apnea-hypopnea syndrome (OSAHS). The severity of OSAHS was positively correlated with COX-2 levels. PMID: 28676625
    7. In conclusion, the present study demonstrated that activation of LXRalpha-ABCA1 axis with a synthetic LXR agonist TO90 exerted a potent protective effect against Abeta induced senescent and inflammatory responses in retinal pigment epithelial cells, suggesting that LXR agonists may be promising therapeutic agents for treating age-related macular degeneration. PMID: 28361293
    8. AMPK activates LXRalpha and ABCA1 expression in human macrophages PMID: 27343431
    9. PPARalpha and LXRalpha may be mediators by which omega3PUFA attenuate bile acid-induced hepatocellular injury PMID: 26756785
    10. Inhibition of Pancreatic Cancer Cell-Induced Paracrine Hedgehog Signaling by Liver X Receptor Agonists and Oxy16, a Naturally Occurring Oxysterol PMID: 27490478
    11. Data identify LXR as an important factor in early-pregnancy lipogenesis that is also necessary to protect against abnormalities in fetoplacental lipid homeostasis. PMID: 28420650
    12. data suggest that ASXL3 is another corepressor of LXRalpha, promoting to the regulation of lipid homeostasis PMID: 25450400
    13. results indicated that LXRalpha plays a specific and important role in activation of TH by regulating D1, and that LXRalpha binds to and regulates the hDIO1 promoter, competing with TRbeta on specific sequences within the promoter. PMID: 28617824
    14. GW3965 significantly increases the expression of liver X nuclear receptor beta (LXRbeta) mRNA, while the liver X nuclear receptor alpha (LXRalpha( mRNA expression did not change a lot, and sensitizes gefitinib by inhibiting NF-kappa B (NF-kappaB) activation. PMID: 28178657
    15. Transactivation assays showed that MCFA activated LXRa, whereas long-chain FA caused no effect. Our results suggest that LXRa functions as a receptor for saturated FA or acyl-CoA of C10 and C12 in length. PMID: 28011707
    16. We demonstrated that LXR stimulation decreases mRNA and protein expression of FLOT2 and DHHC5 in MCF-7 cells. LXR stimulation also reduces Akt phosphorylation and its localization at the plasma membrane PMID: 28739689
    17. The effects of LXR agonist on interleukin-8 (IL-8) secretion and nuclear factor-kappa B (NF-kappaB) activation in human umbilical vein endothelial cells (HUVECs), is reported. PMID: 27489081
    18. Collectively, these findings demonstrate that LXRalpha activation induces 17beta-HSD13 expression in a SREBP-1c-dependent manner. PMID: 28270440
    19. mutant NR1H3 (LXRA) alters gene expression profiles, suggesting a disruption in transcriptional regulation as one of the mechanisms underlying Multiple Sclerosis pathogenesis. PMID: 27253448
    20. Data show that the synthetic liver X receptors (LXRs) agonist T0901317 promoted cytokines IL-1beta, IL-6 and TNFalpha mRNA degradation, destabilized TNFalpha mRNA through its 3'-untranslated region, and increased the expression of tristetraprolin (TTP). PMID: 28119310
    21. RXRalpha negatively regulates the transcription and expression by directly binding to the RARE in the promoter of Cx43 PMID: 26991262
    22. Serum and placental LXR-alpha and endoglin levels were significantly higher in patients with preeclampsia than those in control group (P<0.05, each). PMID: 27736929
    23. this study shows that the anti-inflammatory effect of platycodin D in IL-1beta-stimulated chondrocytes is mediated by activating LXR-a PMID: 27743553
    24. results indicated that down-regulation of LXRalpha was shown to suppress invasion and EMT of gastric cancer cells PMID: 28091828
    25. this study shows that show that H2O2 exerts a dual regulation on mRNA expression of LXRalpha and its target genes PMID: 27351826
    26. combined PPARgamma C1431T, PGC-1alpha G482S, and LXRalpha -115G/A polymorphisms increase the risk of coronary artery disease and predicted the severity of coronary atherosclerosis in Thais PMID: 27016616
    27. Distinct gene regulatory programs define the inhibitory effects of liver X receptors, NR1H2/NR1H3 and PPARG on cancer cell proliferation. PMID: 27401066
    28. these data show that YXS is effective in mitigating MI/R injury by suppressing mitochondrial mediated apoptosis and oxidative stress and by upregulating LXRalpha, thereby providing a rationale for future clinical trials and clinical applications PMID: 26964694
    29. decreased expression of miR-155 in the peripheral blood may be utilized as a potential novel biomarker for non-alcoholic fatty liver disease screening mainly by targeting LXRalpha. PMID: 27832630
    30. The anti-inflammatory effects of platelet-derived microparticles in human plasmacytoid dendritic cells involve liver X receptor activation. PMID: 26635040
    31. these data identify a new mechanism of LXR regulation that involves TIPARP, ADP-ribosylation and MACROD1. PMID: 26814197
    32. Intestinal activation of LXR reduces the production of chylomicrons by a mechanism dependent on the apical localization of SR-B1. PMID: 26602218
    33. Suggest that blocking cholesterol deposition and inhibiting the LXRalpha pathway-induced inflammatory response might be one of the main mechanisms by which anthocyanins exert their protective effects in diabetic nephropathy. PMID: 26379423
    34. LXR-alpha might downregulate S1PR2 expression via miR-130a-3p in quiescent HUVECs. Stimulation of TNF-alpha attenuates the activity of LXR-alpha and results in enhanced S1PR2 expression. PMID: 26669941
    35. Lipoxin A4 increases ABCA1 expression and promotes cholesterol efflux through LXRalpha pathway in THP-1 macrophage-derived foam cells. PMID: 26261553
    36. These results identify LXRalpha as a key cardiac transcriptional regulator that helps orchestrate an adaptive metabolic response to chronic cardiac stress. PMID: 26160456
    37. Studied the role of LXRalpha with Wnt/beta-catenin signaling in adipogenesis of MSCs. PMID: 26595172
    38. In conclusion, our data indicate that HNF-4alpha may have a wider role in cell and plasma cholesterol homeostasis by controlling the expression of LXRalpha in hepatic cells. PMID: 26692490
    39. Propofol up-regulates expression of ABCA1, ABCG1, and SR-B1 through the PPARgamma/LXRalpha pathway in THP-1 macrophage-derived foam cells. PMID: 25600616
    40. The rs12221497 polymorphism in the LXRalpha gene was associated with the susceptibility to stroke in a Han Chinese population. PMID: 25867319
    41. Data show that menin, encoded by the MEN1 gene, inhibits the transcriptional activity of nuclear receptor liver X receptor alpha (LXRalpha). PMID: 25962847
    42. the rsl2221497 polymorphism in the LXRalphagene was associated with the susceptibility to stroke in a Chinese population. PMID: 25729942
    43. treatment with Ang-(1-7) promoted cholesterol efflux in Ang II-treated THP-1 macrophages, partly through inactivation of p38 and JNK signaling and by inducing the expression of PPARg and LXRa. PMID: 25779847
    44. LXRb is the dominant isoform in the rat myocardium and the expression of both LXR isoforms (LXRa and LXRb) did not change after administration of T0901317 PMID: 25659329
    45. The data indicate a direct inhibitory interaction of polyunsaturated fatty acids with LXRalpha, a consequent reduction of SREBP-1 and of its binding to SCD1 promoter. PMID: 25264165
    46. NR1H3 accelerates hepatic differentiation through an HNF4alpha-dependent reciprocal network. PMID: 25073010
    47. This study provides the first evidence to show LXR activation reduces cadmium-induced apoptotic cell death of human renal proximal tubular cells by inhibition of reactive oxygen species production and JNK activation. PMID: 25980575
    48. Activation of LXRs interfered with the release of interleukin-6 from macrophages and, thus, inhibited fibroblast activation and collagen release. PMID: 24618263
    49. ligands selectively regulate placenta gene targets and functional pathways PMID: 25255963
    50. An increase of 55% in LXR-alpha gene expression at RNA level was observed in Atorvastatin + 22-R hydroxycholestrol compared to 24% in Ascorbic acid + 22-ROH cholesterol. PMID: 25283515

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Nuclear hormone receptor family, NR1 subfamily
  • 组织特异性：
    Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
  • 数据库链接：

    HGNC: 7966

    OMIM: 602423

    KEGG: hsa:10062

    STRING: 9606.ENSP00000387946

    UniGene: Hs.438863