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  4. Recombinant Human One cut domain family member 2 (ONECUT2)

Recombinant Human One cut domain family member 2 (ONECUT2)

  • 货号：
    CSB-YP016337HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016337HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016337HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016337HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016337HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ONECUT2
  • Uniprot No.：
  • 别名：
    ONECUT2; HNF6B; One cut domain family member 2; Hepatocyte nuclear factor 6-beta; HNF-6-beta; One cut homeobox 2; Transcription factor ONECUT-2; OC-2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-504
  • 氨基酸序列
    MKAAYTAYRC LTKDLEGCAM NPELTMESLG TLHGPAGGGS GGGGGGGGGG GGGGPGHEQE LLASPSPHHA GRGAAGSLRG PPPPPTAHQE LGTAAAAAAA ASRSAMVTSM ASILDGGDYR PELSIPLHHA MSMSCDSSPP GMGMSNTYTT LTPLQPLPPI STVSDKFHHP HPHHHPHHHH HHHHQRLSGN VSGSFTLMRD ERGLPAMNNL YSPYKEMPGM SQSLSPLAAT PLGNGLGGLH NAQQSLPNYG PPGHDKMLSP NFDAHHTAML TRGEQHLSRG LGTPPAAMMS HLNGLHHPGH TQSHGPVLAP SRERPPSSSS GSQVATSGQL EEINTKEVAQ RITAELKRYS IPQAIFAQRV LCRSQGTLSD LLRNPKPWSK LKSGRETFRR MWKWLQEPEF QRMSALRLAA CKRKEQEPNK DRNNSQKKSR LVFTDLQRRT LFAIFKENKR PSKEMQITIS QQLGLELTTV SNFFMNARRR SLEKWQDDLS TGGSSSTSST CTKA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator. Activates the transcription of a number of liver genes such as HNF3B.
  • 基因功能参考文献：
    1. Bioinformatics-based prediction, combined with qRT-PCR analysis and Western blotting, identified Onecut2 and SIRT1 as the 2 prominent miR-9 target genes under the conditions tested. In this study, Onecut2 and SIRT1 were confirmed as miR-9 target genes, which will help future investigations to probe in greater detail the role of miR-9 in Nonalcoholic Fatty Liver Disease. PMID: 27756894
    2. Data show that ONECUT2, IGF2BP1, and ANXA2 proteins were confirmed to be microRNA-9 (miR-9) targets and aberrantly upregulated in hepatocellular carcinoma (HCC). PMID: 26547929
    3. our data showed that transcript factor Onecut2 is involved in the EMT, migration and invasion of CRC cells; miR-429 inhibits the initiation of EMT and regulated expression of EMT-related markers PMID: 24402783
    4. Mutations at the -5/-6 site of the F9 promotor account for the majority of hemophilia B Leyden cases and disrupt the binding of ONECUT1 and ONECUT2. PMID: 23472758
    5. OC2 transcription factor is a direct target of T-bet in type-1 T-helper cells. PMID: 18418398
    6. A CpG island proximal to ONECUT2 is frequently methylated in diffuse large B-cell lymphoma (DLBCL). PMID: 18288132

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    CUT homeobox family
  • 数据库链接：

    HGNC: 8139

    OMIM: 604894

    KEGG: hsa:9480

    STRING: 9606.ENSP00000262095

    UniGene: Hs.194725