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  4. Recombinant Human Nucleoside diphosphate kinase A (NME1)

Recombinant Human Nucleoside diphosphate kinase A (NME1)

  • 货号：
    CSB-YP015884HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015884HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015884HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015884HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015884HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NME1
  • Uniprot No.：
  • 别名：
    AWD; AWD, drosophila, homolog of; GAAD; Granzyme A activated DNase; Granzyme A-activated DNase; GZMA activated DNase; Metastasis inhibition factor NM23; NB; NBS; NDK A; NDKA; NDKA_HUMAN; NDP kinase A; NDPK-A; NDPKA; NM23; NM23 long variant, included; nm23-H1; NM23-M1; NM23H1B, included; NME/NM23 nucleoside diphosphate kinase 1; Nme1; NME1-NME2 spliced read-through transcript, included; Non-metastatic cells 1, protein (NM23A) expressed in; Nonmetastatic cells 1, protein expressed in; Nonmetastatic protein 23; Nonmetastatic protein 23, homolog 1; Nucleoside diphosphate kinase A; Tumor metastatic process-associated protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 表达区域：
    1-152
  • 氨基酸序列
    MANCERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF MQASEDLLKE HYVDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFHPEELV DYTSCAQNWI YE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
  • 基因功能参考文献：
    1. NME1 enhanced ALDOC transcription, evidenced by increased expression of ALDOC pre-mRNA and activity of an ALDOC promoter-luciferase module. This is the first study to indicate that NME1 induces transcription through its direct binding to the promoter region of a target gene. PMID: 30396920
    2. NME1 rs16949649 associated with increased susceptibility to gynecological cancer and rs2302254 was linked to reduced gastric cancer risk (Meta-Analysis) PMID: 29525404
    3. Immunohistochemical expression of nm23H1 is not an effective tool to distinguish among the cases of BPH, adenocarcinoma of prostate with and without metastasis. Hence nm23H1 gene does not behave like an antimetastatic gene in prostatic lesions. PMID: 29567887
    4. The positive expression rates of KAI1 and nm23 were significantly lower in laryngeal squamous cell carcinoma than normal laryngeal mucosa. PMID: 29187211
    5. coexistence of high MACC1 and low NM23-H1 expression and tumor budding was associated with short overall survival PMID: 29700912
    6. elevated NDKA was associated with severe characteristics of adenomas (>/=3 lesions, size >/= 1 cm or villous component). Setting specificity to 85%, NDKA showed a sensitivity of 30.19% and 29.82% for advanced adenomas and advanced neoplasia, respectively. PMID: 27222072
    7. This study suggested that EGFR was an important predictive factor for the prognosis of the post-operative patients with colorectal carcinoma TNM stage I-II, and nm23 is important for predicting the prognosis of the patients with stage III-IV; it is better that EGFR and nm23 are as predictor of combination. PMID: 27888614
    8. Results demonstrate that nm23 plays a vital role in decidualization in mice and humans and that nm23 gene expression is hormonally regulated. PMID: 27604954
    9. NM23 might be an indicator of good prognosis in patients with breast cancer, although further researches need to be performed to confirm the prognostic value of NM23. [Meta-analysis; review] PMID: 28161101
    10. The data presented suggest an important role for cytoplasmic IRF6 in regulating the availability or localization of the NME1/2 complex and thus the dynamic behavior of epithelia during lip/palate development. PMID: 28767310
    11. High NME1 expression is associated with well tumor differentiation in Digestive System Neoplasms PMID: 27518571
    12. Hepatitis C Virus E1 protein expression and HCV infection induces pro-metastatic effect on cancer cells which is simultaneous to Nm23-H1 transcriptional down-regulation and Nm23-H1 protein degradation. PMID: 28376369
    13. Meta-analysis showed that low expression of nm23-H1 is associated with poorer prognosis in patients with nasopharyngeal carcinoma, suggesting that it is a prognostic factor and potential biomarker for survival in nasopharyngeal carcinoma. PMID: 28614246
    14. Nm23-H1 nuclear localized mainly in the G2/M phase and the nuclear Nm23-H1 promoted A549 cell proliferation in vitro. PMID: 28442010
    15. Differential regulation of NM23-H1 may corroborate/abrogate EMT depending on the nature of stress, tumor microenvironment and cellular context. PMID: 28216015
    16. large-scale and well-designed studies, which use uniform antibody and criterion of NM23 positive expression, are required to further validate the role of the NM23 in predicting gastric cancer progression. PMID: 28401162
    17. Study reveals that NME1L may perform potent biological roles on the cellular behaviors through the extra N-terminal region as well as hexameric conformation. Because the N-terminal region itself has no effect on NF-kappaB signaling, the dimerization of NME1L is likely a pivotal process to confer the IKKbeta binding ability and subsequent regulation of NF-kappaB signaling on the region. PMID: 27094059
    18. A strong association between NME1 heterozygous genotype and breast cancer risk in Kashmiri population PMID: 27509166
    19. Down-regulation of Dyn1 activity enhances extracellular Nme1 in human colon tumor cell lines. PMID: 27449069
    20. nm23-H1 and MMP-2 may be as an indicator for esophageal cancer metastasis and prognosis PMID: 27592483
    21. Results confirmed that NME1L, but not NME1, is likely responsible for regulating breast cancer cell growth by inhibiting IGF1-stimulated ERK phosphorylation through N-terminal 25 amino acid-mediated interaction with KSR1. PMID: 26565392
    22. CRC patients with NM23-positive tumors had a better prognosis, and thus NM23 expression maybe used as a key prognostic indicator for CRC. PMID: 26634527
    23. NM23 expression is reduced in CRC tissues and low NM23 levels tightly correlate with higher Dukes stages, poorer differentiation grade, and positive lymph node metastases. PMID: 26825905
    24. Fibronectin is an important effector of the motility-suppressing function of NME1 in melanoma cells. PMID: 25808322
    25. Nm23 loss could be associated with a more favorable environment for the development and dissemination of breast cancer PMID: 25321081
    26. Dual functions of NME1 in suppression of cell motility and enhancement of genomic stability in melanoma PMID: 25017017
    27. Regulation of the metastasis suppressor Nm23-H1 by tumor viruses PMID: 25199839
    28. nm23-H1 protein may be an important prognostic factor in peripheral T-cell lymphoma, not otherwise specified;the nm23-H1-positive group had significantly shorter overall survival (Review). PMID: 25501107
    29. The case for extracellular Nm23-H1 as a driver of acute myeloid leukemia (AML) progression PMID: 25119778
    30. Interaction between Nm23 and the tumor suppressor VHL PMID: 24915993
    31. Using the described method for detecting histidine and aspartic acid phosphorylations and our prostate cancer progression cell system, the potential function of NM23-H1 in suppressing metastasis with a two-component regulation system is discussed PMID: 25373728
    32. The metastasis suppressor NME1 regulates expression of genes linked to metastasis and patient outcome in melanoma and breast carcinoma. PMID: 25048347
    33. NM23-H1 may participate in head and neck squamous cell carcinoma cell responses to cisplatin and be considered a potential therapeutic target. PMID: 25277180
    34. NM23H1 gene suppresses hyperplasia and metastasis of prostate cancer, thereby improving the survival rate. PMID: 24858271
    35. Overexpression of Nm23H1 did not affect tumorigenesis in nude mice assays, while overexpression of Nm23H2 enhanced tumor growth with elevated expression of the c-Myc proto-oncogene. PMID: 25748386
    36. NDPK-A was not able to bind to model membranes mimicking the inner leaflet of plasma membrane, suggesting that its in vivo membrane association is mediated by a non-lipidic partner or other partners than the studied phospholipids. PMID: 25010650
    37. Data shows that c-Abl and Arg induce NM23-H1 degradation by increasing expression and activation of cathepsin L and B, which directly cleave NM23-H1 in the lysosome. PMID: 24096484
    38. NME1L is a potent antimetastatic protein and may be a useful weapon in the fight against cancers. PMID: 24811176
    39. findings show NDPKs (NM23-H1/H2/H4) interact with and provide GTP to dynamins, allowing these motor proteins to work with high thermodynamic efficiency for membrane remodeling PMID: 24970086
    40. abnormal lower expression of NME1 in endometriotic stromal cells leads to more secretion of IL-8 and VEGF, up-regulates the level of CD62E and CD105, and leads to angiogenesis of vascular endothelial cells, which promotes development of endometriosis. PMID: 24133580
    41. Abnormally low expression of NME1 in endometrial stromal cells (ESCs) may be involved in the pathogenesis of endometriosis by up-regulating growth, adhesion and invasion of ESCs. PMID: 23856325
    42. Positive expression of Nm23 protein was found in ovarian tissue in 77.7 % cases in BRCA1 mutation carriers and in 90.9 % in the control group. PMID: 23553196
    43. NM23 suppressor protein may have a role in gastric carcinoma pathogenes PMID: 23725501
    44. In laryngeal squamous cell carcinoma patients, the disease recurrence rate correlates inversely with nuclear nm23-H1 expression. PMID: 23768014
    45. Data indicate that the activation of MAPK and PI3K pathways resulted in TGF-beta1 signaling by down-regulating Nm23-H1 expression and up-regulating the expression of TbetaRI and TbetaRII, favoring further activation of multiple signaling pathways. PMID: 23734265
    46. High NM23-H1 expression is associated with radioresistance in nasopharyngeal carcinoma. PMID: 23464856
    47. High NM23 expression is associated with sporadic colorectal cancer. PMID: 23679306
    48. Reduced nm23 immunohistochemical expression is an independent negative prognostic factor for overall survival and progression-free survival in invasive breast cancer. PMID: 23818346
    49. The crystal structure of oxidized Nm23-H1 is presented. It reveals the formation of an intramolecular disulfide bond between Cys4 and Cys145 that triggers a large conformational change that destabilizes the hexameric state. PMID: 23519676
    50. NM23 demonstrated no discriminatory value in the interpretation of lymph node nevus rests. PMID: 23694823

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.
  • 蛋白家族：
    NDK family
  • 组织特异性：
    Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to t
  • 数据库链接：

    HGNC: 7849

    OMIM: 156490

    KEGG: hsa:4830

    STRING: 9606.ENSP00000013034

    UniGene: Hs.463456