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  4. Recombinant Human Nuclear receptor subfamily 0 group B member 2 (NR0B2)

Recombinant Human Nuclear receptor subfamily 0 group B member 2 (NR0B2)

  • 货号：
    CSB-YP624025HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP624025HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP624025HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP624025HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP624025HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NR0B2
  • Uniprot No.：
  • 别名：
    NR0B2; NR0B2_HUMAN; Nr0b2a; Nuclear receptor subfamily 0 group B member 2; Nuclear receptor subfamily 0; group B; member 2a; Orphan nuclear receptor SHP; SHP; SHP-1; Shp1; Small heterodimer partner
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-257
  • 氨基酸序列
    MSTSQPGACP CQGAASRPAI LYALLSSSLK AVPRPRSRCL CRQHRPVQLC APHRTCREAL DVLAKTVAFL RNLPSFWQLP PQDQRRLLQG CWGPLFLLGL AQDAVTFEVA EAPVPSILKK ILLEEPSSSG GSGQLPDRPQ PSLAAVQWLQ CCLESFWSLE LSPKEYACLK GTILFNPDVP GLQAASHIGH LQQEAHWVLC EVLEPWCPAA QGRLTRVLLT ASTLKSIPTS LLGDLFFRPI IGDVDIAGLL GDMLLLR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator that acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with which it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated transcription complex for NEUROD1. Essential component of the liver circadian clock which via its interaction with NR1D1 and RORG regulates NPAS2-mediated hepatic lipid metabolism. Regulates the circadian expression of cytochrome P450 (CYP) enzymes. Represses: NR5A2 and HNF4A to down-regulate CYP2C38, NFLI3 to up-regulate CYP2A5, BHLHE41/HNF1A axis to up-regulate CYP1A2, CYP2E1 and CYP3A11, and NR1D1 to up-regulate CYP2B10, CYP4A10 and CYP4A14.
  • 基因功能参考文献：
    1. results suggest that NR0B2 is a tumor suppressor gene in ccRCC, and that the hypermethylation of promoter region is the main mechanism of its downregulation. PMID: 29989580
    2. DDX3 regulates MTP gene expression and lipid homeostasis through interplay with HNF4 and SHP. PMID: 28128295
    3. The miR-141 was significantly up-regulated in the samples of metastatic tumors compared to localized tumor samples. Tumor samples showed lower SHP mRNA expression levels than BPH samples. PMID: 29562494
    4. The impact of these variants on transactivation of the NR0B2 promoter by NRs known to be regulators of SHP1 expression was assessed in a cell-based reporter gene assay, showing that transactivation by hepatocyte nuclear factor 4alpha and liver receptor homolog-1 was significantly decreased in the presence of the genetic variant NR0B2:c.-594T> PMID: 28873070
    5. Data indicate a functional interaction between small heterodimer partner protein SHP1 and hepatitis C virus (HCV) NS5A protein. PMID: 27661118
    6. This study was performed to test the hypothesis that the SHP gene is a target gene of DEC1. Cotransfection demonstrated that DEC1 repressed the SHP promoter and attenuated the transactivation of the classic circadian activator complex of Clock/Bmal1. PMID: 28797635
    7. The molecular dynamics and structural analysis of the small heterodimer partner in complex with glucocorticoid receptor is described. PMID: 28396564
    8. The finding that overexpression of HIF-1alpha increased the activity of the CYP7A1 promoter suggested that hypoxia decreased the expression of CYP7A1 in a HIF-1-independent manner. PMID: 26521940
    9. Results demonstrated a detrimental effect of Bcl2 and H19 associated with cholestatic liver fibrosis and an indispensable role of Shp to maintain normal liver function. PMID: 26838806
    10. These findings show that POD-1/TCF21 regulates SF-1 and LRH-1 by distinct mechanisms, contributing to the understanding of POD-1 involvement and its mechanisms of action in adrenal and liver tumorigenesis. PMID: 26421305
    11. Overexpression of SHP and activation of AMPK reverses profibrogenic features of HCV-infected cells by decreasing TGF-beta and fibrotic gene expression. PMID: 25976932
    12. Data suggest that LRH1/NR5A2 (liver receptor homologue-1) exhibits phospholipid-mediated allosteric control of protein-protein binding interface in interactions with TIF2 (co-activator; transcription intermediary factor 2) and SHP. PMID: 26553876
    13. At the molecular level, estrogen upregulated hepatic SHP expression through binding to its proximal promoter. In addition, the roles of estrogen were largely blunted in mice with SHP deficiency. PMID: 25720568
    14. These data are suggestive of a role for SHP in controlling NLRP3 inflammasome activation through a mechanism involving interaction with NLRP3 and maintenance of mitochondrial homeostasis. PMID: 25655831
    15. These results establish SHP as a novel antihypertrophic regulator that acts by interfering with GATA6 signaling. PMID: 25015078
    16. E2F1 transcription factor activates early growth response (Egr)-1 promoter which is repressed by SHP and EIA-like inhibitor of differentiation (EID)1. PMID: 24619556
    17. SHP regulates PDCD5-mediated apoptosis in cancer cells PMID: 24343129
    18. SHP repressed HNF4alpha transactivation of CYP2D6 promoter. PMID: 24318876
    19. An unexpected EID1-binding site on SHP is located at the N terminus of the receptor, where EID1 mimics helix H1 of the nuclear receptor ligand-binding domain. PMID: 24379397
    20. These results indicate that the N-terminal domain of SHP is important for its nuclear translocation via HNF4alpha. PMID: 23874642
    21. This study identifies PKCzeta as a novel key upstream regulator of BA-regulated SHP function, revealing the role of Thr-55 phosphorylation in epigenomic regulation of liver metabolism. PMID: 23824184
    22. HNF4alpha and LRH-1 promote active transcription histone marks on the Cyp7a1 promoter that are reversed by FGF19 in a SHP-dependent manner PMID: 23038264
    23. SHP is a downstream factor of BMP2 signaling in odontoblasts and may stimulate dentin matrix mineralization via enhancing the transcriptional activity of Dlx5. PMID: 23010719
    24. Lentivirus-mediated exogenous FXR expression resulted in a marked increase of small heterodimer partner expression, significant repression of liver cancer cell proliferation. PMID: 23042943
    25. Shp is a target for miR-141 and it is downregulated in cultured human PCa cells with the involvement of upregulation of miR-141, which promotes AR transcriptional activity. PMID: 22314666
    26. NR0B2 physically interacted with STAT5 and inhibited STAT5 recruitment on the PEPCK gene promoter. PMID: 22977252
    27. Overexpression of p53 causes rapid SHP protein degradation, which does not require the presence of Mdm2 and is mediated by the proteosome pathway PMID: 22737255
    28. In human hepatocellular carcinoma, increased DNMT1 expression is negatively correlated with SHP levels. PMID: 22362755
    29. The impact of loss of SHP protein may be even more pronounced in fibrolamellar carcinoma than in a typical form of hepatocellular carcinoma. PMID: 22292081
    30. FXR and SHP play a limited role in modulating HBV biosynthesis. PMID: 22171277
    31. control network for Egr-1 expression through a feedback loop between SHP and HNF4alpha. PMID: 21725089
    32. Study provides evidence for nuclear receptor mediated regulation of Dnmt1 expression through ERRgamma and SHP crosstalk. PMID: 21459093
    33. The activity of SHP is also increased by posttranslational methylation at Arg-57 by protein arginine methyltransferase 5 (PRMT5). PMID: 21262773
    34. NR0B2/SHP expression level was significantly decreased in human liver cancer but not in normal liver PMID: 20853064
    35. mutagenesis experiments whose preliminary data suggest the presence of a functional site in SHP as defined by residues Phe96, Arg138 and Arg238 PMID: 20882396
    36. genetic variations in SHP are common among human subjects; the Lys-170 residue plays a key role in controlling SHP ubiquitination and acetylation associated with SHP protein stability and repressive function PMID: 20516075
    37. Mutations of the small heterodimer partner gene is associated with obesity and type 2 diabetes. PMID: 20233523
    38. SHP overexpression decreased the expression of Gli target genes and SHP knockdown increased the expression of these genes. The results suggest that SHP can play a negative role in Hedgehog/Gli1 signalling. PMID: 20175750
    39. polymorphisms in the human SHP1 gene; found no rare SHP1 coding sequence variants that were exclusive to patients with lipodystrophy PMID: 12181644
    40. SHP is able to interact with LXR and to modulate its transcriptional activity. PMID: 12198243
    41. SHP has a role in modulating hepatic glucocorticoid action PMID: 12324453
    42. the relation between genetic variation in SHP and birth weight, adiposity, and insulin levels in three independent populations PMID: 12716767
    43. orphan receptor small heterodimer partner expression is regulated by estrogen receptor alpha PMID: 12842887
    44. Modulation of SHP expression and/or activity in adipose tissue may therefore have significant effects on aromatase expression and estrogen production in breast adipose tissue. PMID: 14593077
    45. basic helix-loop-helix (bHLH) transcription factors, the E2A proteins (E47, E12 and E2/5), activated the human but not the mouse SHP promoter PMID: 14627819
    46. results suggest that bile acids negatively regulate the human angiotensinogen gene through the inhibitory effect of small heterodimer partner on hepatocyte nuclear factor-4 PMID: 14672953
    47. Acts as a novel corepressor for basic helix-loop-helix transcription factor BETA2/NeuroD PMID: 14752053
    48. At diagnosis, methylation of SHP1 occurred more frequently in acute myeloid leukaemia than acute lymphoblastic leukaemia. Frequent methylation of SHP1, but not SOCS1, may be important in the pathogenesis, but not prognosis, of acute leukaemias PMID: 14762685
    49. orphan nuclear receptor small heterodimer partner promoter is regulated by sterol regulatory element binding protein-1 PMID: 15123650
    50. PGC-1alpha mediates the ligand-dependent activation of FXR and transcription of SHP gene. PMID: 15202934

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  • 相关疾病：
    Obesity (OBESITY)
  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Colocalizes with NEUROD1 in the nucleus.
  • 蛋白家族：
    Nuclear hormone receptor family, NR0 subfamily
  • 组织特异性：
    Liver. Low levels of expression were detected in heart and pancreas.
  • 数据库链接：

    HGNC: 7961

    OMIM: 601665

    KEGG: hsa:8431

    STRING: 9606.ENSP00000254227

    UniGene: Hs.427055