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  4. Recombinant Human Nuclear receptor coactivator 4 (NCOA4)

Recombinant Human Nuclear receptor coactivator 4 (NCOA4)

  • 货号：
    CSB-YP015542HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015542HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015542HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015542HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015542HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NCOA4
  • Uniprot No.：
  • 别名：
    70 kDa androgen receptor activator; 70 kDa androgen receptor coactivator; 70 kDa AR activator; 70 kDa AR-activator; Androgen receptor coactivator 70 kD; Androgen receptor coactivator 70 kDa protein; Androgen receptor-associated protein of 70 kDa; ARA 70; ARA70; DKFZp762E1112; ELE 1; ELE1; ELE1/ret TK; NCOA 4; NCoA-4; NCOA4; NCOA4_HUMAN; Nuclear receptor coactivator 4; Papillary thyroid carcinoma 3; PTC 3; PTC3; RET activating gene ELE1; Ret activating protein ELE1; Ret-activating protein ELE1; RFG
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-614
  • 氨基酸序列
    MNTFQDQSGS SSNREPLLRC SDARRDLELA IGGVLRAEQQ IKDNLREVKA QIHSCISRHL ECLRSREVWL YEQVDLIYQL KEETLQQQAQ QLYSLLGQFN CLTHQLECTQ NKDLANQVSV CLERLGSLTL KPEDSTVLLF EADTITLRQT ITTFGSLKTI QIPEHLMAHA SSANIGPFLE KRGCISMPEQ KSASGIVAVP FSEWLLGSKP ASGYQAPYIP STDPQDWLTQ KQTLENSQTS SRACNFFNNV GGNLKGLENW LLKSEKSSYQ KCNSHSTTSS FSIEMEKVGD QELPDQDEMD LSDWLVTPQE SHKLRKPENG SRETSEKFKL LFQSYNVNDW LVKTDSCTNC QGNQPKGVEI ENLGNLKCLN DHLEAKKPLS TPSMVTEDWL VQNHQDPCKV EEVCRANEPC TSFAECVCDE NCEKEALYKW LLKKEGKDKN GMPVEPKPEP EKHKDSLNMW LCPRKEVIEQ TKAPKAMTPS RIADSFQVIK NSPLSEWLIR PPYKEGSPKE VPGTEDRAGK QKFKSPMNTS WCSFNTADWV LPGKKMGNLS QLSSGEDKWL LRKKAQEVLL NSPLQEEHNF PPDHYGLPAV CDLFACMQLK VDKEKWLYRT PLQM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma.
  • 基因功能参考文献：
    1. Thyroid hormone receptor beta and NCOA4 regulate terminal erythrocyte differentiation. PMID: 28864529
    2. The H-ferritin could bind up to 24 NCOA4(383-522) fragments forming highly stable and insoluble complexes. The binding was partially inhibited only by Fe(II) among the various divalent metal ions analyzed. The iron-dependent, highly-specific formation of the remarkably stable H-ferritin-NCOA4 complex shown in this work may be important for the characterization of the mechanism of ferritinophagy. PMID: 28754384
    3. the ATG5-ATG7-NCOA4 autophagic pathway has a role in ferroptosis PMID: 27245739
    4. Ubiquitin-dependent NCOA4 turnover is promoted by excess iron and involves an iron-dependent interaction between NCOA4 and the HERC2 ubiquitin ligase. PMID: 26436293
    5. Studies indicate nuclear receptor coactivator 4 (NcoA4) as a coactivator for a variety of nuclear receptors. PMID: 22562579
    6. provide a new mechanism for selective autophagy of ferritin and reveal a previously unappreciated role for autophagy and NCOA4 in the control of iron homeostasis in vivo PMID: 25327288
    7. RET/PTC3 gene rearrangements are the most prevalent form of rearrangements in papillary thyroid carcinomas of Chennai population. PMID: 24957039
    8. NCOA4 acts as a regulator of DNA replication origins that helps prevent inappropriate DNA synthesis and replication stress. PMID: 24910095
    9. identification of NCOA4 as a selective cargo receptor for autophagic turnover of ferritin (ferritinophagy), which is critical for iron homeostasis, and provides a resource for further dissection of autophagosomal cargo-receptor connectivity PMID: 24695223
    10. mechanism dissection shows that KLK2 may cooperate with the AR coregulator, ARA70, to enhance AR transactivation that may result in alteration of prostate cancer formation PMID: 24122203
    11. Data indicate that the increase in prostate cancer (PC) risk associated with rs10993994:C>T is likely mediated by the variant's effect of MSMB-encoded protein PSP94 expression; however, this effect does not extend to NCOA4 in the data. PMID: 22887727
    12. Computational network analysis reveals that the MSMB gene is functionally connected to NCOA4 and the androgen receptor signaling pathway. The data provide an example of how GWAS-associated variants may have multiple genetic and epigenetic effects PMID: 22661295
    13. Suppression of MSMB expression or NCOA4 overexpression promotes anchorage-independent growth of prostate epithelial cells. PMID: 21085629
    14. ARA70alpha functions as a tumor suppressor gene PMID: 20167864
    15. Screening for BRAF, RET, KRAS, NRAS, and HRAS mutations, as well as RET-PTC1 and RET-PTC3 rearrangements, was performed on cases of Hashimoto thyroiditis with a dominant nodule PMID: 20012784
    16. Potent mitogenicity of the RET/PTC3 oncogene correlates with its prevalence in tall-cell variant of papillary thyroid carcinoma. PMID: 11786418
    17. Domain interactions between coregulator ARA(70) and the androgen receptor (AR); structure activity relationship PMID: 11818501
    18. ARA70 has a role in enhancing the androgen receptor during testicular development PMID: 12068007
    19. role in reducing agonist activity of antiandrogens PMID: 12649293
    20. causes increases in nuclear NF-kappaB activity and secretion of MCP-1 and GM-CSF; studies implicate oncogene-induced cytokine-signaling pathways in a new mechanism linking inflammation with cancer PMID: 12881713
    21. ARA70 was identifed as a coactivator of PPARalpha in the prostate cancer cell line DU145. In the adrenal cell line Y1, ARA70 behaved as a repressor of PPARalpha while still able to coactivate PPARgamma. PMID: 12897377
    22. The refolding and one-step purification of ARA70 from inclusion bodies over-expressed in Escherichia coli is reported. PMID: 15642480
    23. ARA70 is a coactivator for estrogen receptor alpha (ERalpha) and may represent a functional link between ERalpha/androgen receptor (AR) modulating their cross-talk in models of estrogen signaling in MCF-7 and HeLa cells PMID: 15772083
    24. Together, ARA54 and ARA70 modulate vitamin D receptor transactivation, and the competition for ARA70 mediates the suppressive affect of androgen-androgen receptor on vitamin D receptor transactivation. PMID: 15805579
    25. a functional interaction between NcoA4 and AhR that may alter AhR activity to affect disease development and progression is demonstrated PMID: 16762319
    26. Expression of androgen receptor co-regulators in the testes of men with azoospermia. PMID: 17919607
    27. in prostate cancer cell lines, ARA70beta localized to the cytosol, whereas ARA70alpha was found in the nucleus, supporting the notion of their dissimilar functions PMID: 18091327
    28. ARA70 beta is a regulator of tumor cell growth and metastasis by affecting gene expression PMID: 18156210

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  • 相关疾病：
    A chromosomal aberration involving NCOA4 is found in papillary thyroid carcinomas (PTCs). Inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene.
  • 组织特异性：
    Widely expressed. Also detected in adipose tissues and in different cell lines. Isoform Beta is only expressed in testis.
  • 数据库链接：

    HGNC: 7671

    OMIM: 601984

    KEGG: hsa:8031

    STRING: 9606.ENSP00000395465

    UniGene: Hs.643658