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  4. Recombinant Human Nuclear distribution protein nudE-like 1 (NDEL1)

Recombinant Human Nuclear distribution protein nudE-like 1 (NDEL1)

  • 货号：
    CSB-EP872414HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    NDEL1
  • Uniprot No.：
  • 别名：
    A. nidulans; DKFZp451M0318; ENDOOLIGOPEPTIDASE A; EOPA; MITAP 1; MITAP1; Mitosin associated protein 1; Mitosin associated protein MITAP1; Mitosin-associated protein 1; Ndel 1; NDEL1; NDEL1_HUMAN; Nuclear distribution gene E like homolog 1; Nuclear distribution protein nudE like 1; Nuclear distribution protein nudE-like 1; NUDE like protein; NudE nuclear distribution gene E homolog like 1 A. nidulans; NudE nuclear distribution gene E homolog like 1; NUDEL; Protein Nudel
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of BC026101
  • 来源：
    E.coli
  • 分子量：
    65.4kDa
  • 表达区域：
    1-345aa
  • 氨基酸序列
    MDGEDIPDFSSLKEETAYWKELSLKYKQSFQEARDELVEFQEGSRELEAELEAQLVQAEQRNRDLQADNQRLKYEVEALKEKLEHQYAQSYKQVSVLEDDLSQTRAIKEQLHKYVRELEQANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERQQEVTRKSAPSSPTLDCEKMDSAVQASLSLPATPVGKGTENTFPSPKAIPNGFGTSPLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQASRKSYISGNVNCGVLNGNGTKFSRSGHTSFFDKGAVNGFDTAPPPPGLGSSRPSSAPGMLPLSV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts.
  • 基因功能参考文献：
    1. Results demonstrate that Ndel1 interacts with Tara to regulate cell movement revealing a novel role of the Ndel1-Tara complex in actin reorganization during cell movement. PMID: 27546710
    2. results highlight S-Nitrosylation as a key activity-dependent mechanism underlying neonatal brain maturation and suggest that reduction of S-Nitrosylation of NDEL1 acts as a pathological factor mediating neurodevelopmental abnormalities caused by maternal alcohol exposure PMID: 27371763
    3. disrupting DISC1/Ndel1 complex formation prolongs mitotic length and interferes with cell-cycle progression in human cells, and it causes cell-cycle deficits of radial glial cells in the embryonic mouse cortex and human forebrain organoids PMID: 29103808
    4. Study showed that Ndel1 enzyme activity is a complex trait influenced by many different genetic variants that may contribute to schizophrenia physiopathology PMID: 26851141
    5. This study observed a significantly increased myelin basic protein (MBP) and nuclear distribution protein nudE-like 1 (NDEL1) mRNA levels in FEP patients compared with controls. PMID: 26476704
    6. The Plasma Ndel1 enzyme activity is reduced in patients with schizophrenia. PMID: 23388542
    7. Short (DISC)1 splice variants show reduced or no binding to nudE nuclear distribution E homolog (NDEL)1 and phosphodiesterase (PDE)4B proteins, but fully interact with FEZ1 and glycogen synthase kinase 3 (GSK3)beta. PMID: 22832604
    8. Nudel markedly promotes the HSPC300-WAVE2 interaction. PMID: 22453242
    9. Cytosolic mfGbeta is recruited to dynein by Nudel and transported to the centrosome for rapid sequestration and degradation. PMID: 22430153
    10. Studies indicate that binding of dynactin, LIS1 and NudEL regulate cytoplasmic dynein motor activity. PMID: 22373868
    11. DISC1 forms octamers via dimers as building blocks. S704C forms higher-order oligomers, without affecting its affinity with NDEL1 PMID: 21998303
    12. Nuclear distribution element-like 1 (Ndel1 or Nudel) was firstly described as a regulator of the cytoskeleton in microtubule and intermediate filament dynamics and microtubule-based transport. PMID: 21948775
    13. Evidence of statistical epistasis between DISC1, CIT and NDEL1 impacting risk for schizophrenia: biological validation with functional neuroimaging. PMID: 20084519
    14. The truncated monomeric form of LIS1 had little effect on dynein motility, but an artificial dimer of truncated LIS1 suppressed dynein motility, which was restored by the N-terminal fragment of NDEL1. PMID: 21036906
    15. NDEL1 is essential for a specific late step of neuronal migration: entry into the target lamina. PMID: 21092859
    16. This data supports a physiological role for the endooligopeptidase activity of Ndel1 and suggests it plays a key role during neurite outgrowth. PMID: 20462516
    17. Findings suggest that physiological functions of LIS1 and NDEL1 in neurons have been ascribed for proteins fundamentally required for cell cycle progression and control. PMID: 20168084
    18. palmitoylated Ndel1 reduced cytoplasmic dynein activity as judged by Golgi distribution, VSVG and short microtubule trafficking, transport of endogenous Ndel1 and LIS1 from neurite tips to the cell body and neuronal migration PMID: 19927128
    19. disease mutant form of Disrupted-in-Schizophrenia-1 fails to bind NUDEL PMID: 12506198
    20. results point to the importance of Nudel-Lis1 interaction for the dynein activity in M phase and to a possible role of Nudel phosphorylation as facilitating such interaction. PMID: 12556484
    21. DISC1 inhibits NUDEL-oligopeptidase activity in a competitive fashion. PMID: 15728732
    22. NDEL1 is essential for mitotic cell division and neuronal migration not only via regulation of cytoplasmic dynein function but also by modulation of katanin p60 localization and function. PMID: 16203747
    23. Aurora-A-mediated phosphorylation of NDEL1 is essential for centrosomal separation and centrosomal maturation and for mitotic entry PMID: 17060449
    24. These results suggest a dual role of kinetochore Nudel: it activates dynein-mediated protein transport and, when interacting with both mitosin and dynein, stabilizes kinetochore dynein/dynactin. PMID: 17494871
    25. Ndel1-deficient cells enter anaphase in a timely manner but lagging chromosomes then manifest. PMID: 17600710
    26. crystallographic study of two fragments of the coiled-coil domain of Ndel1, one of which reveals contiguous high-quality electron density for residues 10-166, the longest such structure reported by X-ray diffraction at high resolution PMID: 17997972
    27. Our work uncovers a unique regulatory mechanism of MT organization by PP4c through its targets Cdk1 and NDEL1 via regulation of katanin p60 distribution. PMID: 18347064
    28. For DISC1-related sporadic psychiatric disease, impaired cellular control over self-association of DISC1 leads to excessive multimerization and subsequent formation of detergent-resistant aggregates, with loss of ligand binding, exemplified by NDEL1. PMID: 18400883
    29. NDEL1 regulates cytoplasmic dynein and involved in the regulation of microtubule organization, and becomes the target of various kinases and phosphatases. PMID: 18421979
    30. NDEL1 significantly influences risk for schizophrenia via an interaction with DISC1. PMID: 18469341
    31. Data show that LIS1 suppresses the motility of cytoplasmic dynein on microtubules, whereas NDEL1 releases the blocking effect of LIS1 on cytoplasmic dynein. PMID: 18784752
    32. NDEL1 protein interacts directly with Cyclic Nucleotide Phosphodiesterases, Type 4 and its isoforms and interaction of NDEL1 protein with phosphodiesterase isoform is uniquely disrupted by elevation of intracellular cyclic AMP levels. PMID: 18845247
    33. Nudel regulates microtubule organization in part by facilitating assembly of the lamin B spindle matrix in a dynein-dependent manner. PMID: 19198602
    34. These results suggest a novel mechanism for selective reinforcement of nascent adhesions via interplays of Nudel and FAK with paxillin to facilitate cell migration. PMID: 19492042
    35. These results indicate an antagonistic effect of alpha1, alpha2 and Ndel1 for Lis1 binding, probably to modulate dynein functions in vivo. PMID: 19622634
    36. There was no strong evidence for association with NUDEL in schizophrenia. PMID: 19632097

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    NudE family
  • 组织特异性：
    Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
  • 数据库链接：

    HGNC: 17620

    OMIM: 607538

    KEGG: hsa:81565

    STRING: 9606.ENSP00000333982

    UniGene: Hs.372123