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  4. Recombinant Human Terminal nucleotidyltransferase 4B (TENT4B)

Recombinant Human Terminal nucleotidyltransferase 4B (TENT4B)

  • 中文名称：
    人PAPD5重组蛋白
  • 货号：
    CSB-YP843329HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PAPD5重组蛋白
  • 货号：
    CSB-EP843329HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PAPD5重组蛋白
  • 货号：
    CSB-BP843329HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PAPD5重组蛋白
  • 货号：
    CSB-MP843329HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PAPD5
  • Uniprot No.：
  • 别名：
    TENT4B; GLD4; PAPD5; TRF4-2; TUT3; Terminal nucleotidyltransferase 4B; Non-canonical poly(A) RNA polymerase PAPD5; EC 2.7.7.19; PAP-associated domain-containing protein 5; Terminal guanylyltransferase; EC 2.7.7.-; Terminal uridylyltransferase 3; TUTase 3; Topoisomerase-related function protein 4-2; TRF4-2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-572
  • 氨基酸序列
    MYRSGERLLG SHALPAEQRD FLPLETTNNN NNHHQPGAWA RRAGSSASSP PSASSSPHPS AAVPAADPAD SASGSSNKRK RDNKASGGRA AGGGRADGGG VVYSGTPWKR RNYNQGVVGL HEEISDFYEY MSPRPEEEKM RMEVVNRIES VIKELWPSAD VQIFGSFKTG LYLPTSDIDL VVFGKWENLP LWTLEEALRK HKVADEDSVK VLDKATVPII KLTDSFTEVK VDISFNVQNG VRAADLIKDF TKKYPVLPYL VLVLKQFLLQ RDLNEVFTGG IGSYSLFLMA VSFLQLHPRE DACIPNTNYG VLLIEFFELY GRHFNYLKTG IRIKDGGSYV AKDEVQKNML DGYRPSMLYI EDPLQPGNDV GRSSYGAMQV KQAFDYAYVV LSHAVSPIAK YYPNNETESI LGRIIRVTDE VATYRDWISK QWGLKNRPEP SCNGPVSSSS ATQSSSSDVD SDATPCKTPK QLLCRPSTGN RVGSQDVSLE SSQAVGKMQS TQTTNTSNST NKSQHGSARL FRSSSKGFQG TTQTSHGSLM TNKQHQGKSN NQYYHGKKRK HKRDAPLSDL CR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Terminal nucleotidyltransferase that catalyzes preferentially the transfert of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation. Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro). Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1. Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion. Mediates 3' adenylation of the microRNA MIR21 followed by its 3'-to-5' trimming by the exoribonuclease PARN leading to degradation. Mediates 3' adenylation of H/ACA box snoRNAs (small nucleolar RNAs) followed by its 3'-to-5' trimming by the exoribonuclease PARN which enhances snoRNA stability and maturation.
  • 基因功能参考文献：
    1. TENT4A and TENT4B produce a mixed tail that shields mRNA from rapid deadenylation. PMID: 30026317
    2. A novel post-transcriptional regulatory network involving carbohydrate metabolism and glucose homeostasis mediated by GLD4. PMID: 28383716
    3. We analyzed the interactions of the human TRAMP-like proteins, PAPD5, ZCCHC7, and MTR4, with the nuclear exosome. PAPD5 and ZCCHC7 exhibited mutual interactions in presence of the exosome catalytic subunit RRP6, whereas MTR4 was dispensable for their assembly PMID: 27434818
    4. PAPD5 and PARN mediate degradation of oncogenic miRNA miR-21. PMID: 25049417
    5. PAPD5 catalyzes the polyadenylation of different types of RNA substrates in vitro. PMID: 21788334

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  • 亚细胞定位：
    Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Predominantly expressed in the cytoplasm (PubMed:18172165).
  • 蛋白家族：
    DNA polymerase type-B-like family
  • 数据库链接：

    HGNC: 30758

    OMIM: 605540

    KEGG: hsa:64282

    STRING: 9606.ENSP00000396995

    UniGene: Hs.514342