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  4. Recombinant Human Nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2)

Recombinant Human Nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2)

  • 中文名称：
    人NMNAT2重组蛋白
  • 货号：
    CSB-YP866332HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人NMNAT2重组蛋白
  • 货号：
    CSB-EP866332HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人NMNAT2重组蛋白
  • 货号：
    CSB-EP866332HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人NMNAT2重组蛋白
  • 货号：
    CSB-BP866332HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人NMNAT2重组蛋白
  • 货号：
    CSB-MP866332HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NMNAT2
  • Uniprot No.：
  • 别名：
    C1orf15; KIAA0479; MGC2756; NaMN adenylyltransferase 2; Nicotinamide mononucleotide adenylyltransferase 2; Nicotinamide nucleotide adenylyltransferase 2; Nicotinate-nucleotide adenylyltransferase 2; NMN adenylyltransferase 2; NMNA2_HUMAN; NMNAT 2; Nmnat2; PNAT 2; PNAT2; Pyridine nucleotide adenylyltransferase 2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-307
  • 氨基酸序列
    MTETTKTHVI LLACGSFNPI TKGHIQMFER ARDYLHKTGR FIVIGGIVSP VHDSYGKQGL VSSRHRLIMC QLAVQNSDWI RVDPWECYQD TWQTTCSVLE HHRDLMKRVT GCILSNVNTP SMTPVIGQPQ NETPQPIYQN SNVATKPTAA KILGKVGESL SRICCVRPPV ERFTFVDENA NLGTVMRYEE IELRILLLCG SDLLESFCIP GLWNEADMEV IVGDFGIVVV PRDAADTDRI MNHSSILRKY KNNIMVVKDD INHPMSVVSS TKSRLALQHG DGHVVDYLSQ PVIDYILKSQ LYINASG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Nicotinamide/nicotinate-nucleotide adenylyltransferase that acts as an axon maintenance factor. Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NaAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Axon survival factor required for the maintenance of healthy axons: acts by delaying Wallerian axon degeneration, an evolutionarily conserved process that drives the loss of damaged axons.
  • 基因功能参考文献：
    1. analyses found that rs10494561, located in the intron region within NMNAT2, was associated with the severity of the prodromal symptoms of psychosis implicitly, mediated through the volume of the left hemisphere of the superior frontal region PMID: 28544218
    2. Data indicate a chaperone function of nicotinamide mononucleotide adenylyl transferase 2 (NMNAT2), independent from its enzymatic activity, and NMNAT2 complexes with heat shock protein 90 (HSP90) to refold aggregated protein substrates. PMID: 27254664
    3. We confirmed association at NMNAT2 and identified independent SMG7 association with systemic lupus erythematosus in European American and Amerindian/Hispanic ancestries. PMID: 26783109
    4. NMNAT2 might participate in tumorigenesis of CRC. PMID: 27218101
    5. Knockdown of NMNAT-2 significantly reduces cellular NAD(+) levels and protects cells from p53-dependent cell death upon DNA damage, suggesting an important functional role of NMNAT-2 in p53-mediated signaling. PMID: 24552824
    6. Nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2) is essential for axon growth and survival. PMID: 24840802
    7. Overexpression of NMNAT2 in colorectal cancer cells renders them sensitive to Tiazofurin antineoplastic action. PMID: 23764899
    8. affects tau phosphorylation by regulating PP2A activity PMID: 23579329
    9. Decreased endogenous NMNAT2 function caused by reduced CREB signaling during pathological insults may be one of underlying mechanisms for neuronal death in tauopathies PMID: 22027994
    10. that overexpression of Nmnat2 in M-cells significantly delayed axon degeneration in vivo PMID: 20857515
    11. Nmnat2 is a neuronal protein peripherally attached to membranes via palmitoylation and suggest that Nmnat2 is transported to synaptic terminals via an endosomal pathway. PMID: 20943658
    12. analysis of isoform-specific targeting and interaction domains in human nicotinamide mononucleotide adenylyltransferases PMID: 20388704
    13. cloning, isolation and kinetics PMID: 12359228
    14. Nicotinamide 5'-mononucleotide adenylyltransferase-2 is markedly expressed in the islets of Langerhans. PMID: 14516279
    15. NMNAT1 is a nuclear protein, whereas NMNAT2 and -3 are localized to the Golgi complex and the mitochondria PMID: 16118205
    16. ATP binds before NMN with Golgi apparatus NMNAT2. NMNH conversion to NADH by NMNAT1 and NMNAT3 occurs at similar rates, conversion by NMNAT2 is much slower. PMID: 17402747

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  • 亚细胞定位：
    Golgi apparatus membrane; Lipid-anchor. Cytoplasmic vesicle membrane; Lipid-anchor. Cytoplasm. Cell projection, axon.
  • 蛋白家族：
    Eukaryotic NMN adenylyltransferase family
  • 组织特异性：
    Highly expressed in brain, in particular in cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Also found in the heart, skeletal muscle, pancreas and islets of Langerhans.
  • 数据库链接：

    HGNC: 16789

    OMIM: 608701

    KEGG: hsa:23057

    STRING: 9606.ENSP00000287713

    UniGene: Hs.497123