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  4. Recombinant Human Nicotinamide N-methyltransferase (NNMT)

Recombinant Human Nicotinamide N-methyltransferase (NNMT)

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  • 货号：
    CSB-EP015906HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    NNMT
  • Uniprot No.：
  • 别名：
    EC 2.1.1.1; Nicotinamide N methyltransferase; Nicotinamide N-methyltransferase; NNMT; NNMT_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    56.6kDa
  • 表达区域：
    1-264aa
  • 氨基酸序列
    MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway. Plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3. In a developmental context, contributes to low levels of the repressive histone marks that characterize pluripotent embryonic stem cell pre-implantation state. Acts as a metabolic regulator primarily on white adipose tissue energy expenditure as well as hepatic gluconeogenesis and cholesterol biosynthesis. In white adipocytes, regulates polyamine flux by consuming S-adenosyl-L-methionine which provides for propylamine group in polyamine biosynthesis, whereas by consuming nicotinamide controls NAD(+) levels through the salvage pathway. Via its product N1-methylnicotinamide regulates protein acetylation in hepatocytes, by repressing the ubiquitination and increasing the stability of SIRT1 deacetylase. Can also N-methylate other pyridines structurally related to nicotinamide and play a role in xenobiotic detoxification.
  • 基因功能参考文献：
    1. TGF-beta1 is significantly overexpressed in tumor tissue samples of clear cell Renal cell carcinoma patients. TGF-beta1 up-regulation could be responsible for the high levels of NNMT observed in clear cell Renal cell carcinoma tissues. PMID: 29974846
    2. Our data indicate that NNMT is a promising biomarker that could be used to support the early and noninvasive diagnosis of Bladder cancer PMID: 29148015
    3. Here we explored the association between NNMT gene polymorphisms and obesity. The subjects were recruited from male Chinese Han college student... the variation of the tagSNP, rs10891644, is significantly associated with obesity and the GT carriers are the susceptible population PMID: 29075643
    4. our results suggest that the ZEB1/NNMT signaling axis induces phenotypic and metabolic plasticity, as well as mesenchymal gene expression in ovarian cancer cells upon chronic glucose deprivation PMID: 28412735
    5. We demonstrate that NNMT outcompetes leucine carboxyl methyl transferase 1 (LCMT1) for methyl transfer from principal methyl donor SAM in biological systems. Inhibiting NNMT increased the availability of methyl groups for LCMT1 to methylate PP2A, resulting in the inhibition of oncogenic serine/threonine kinases (STK). PMID: 27810903
    6. High expression level of NNMT is associated with pancreatic cancer. PMID: 26942567
    7. This finding, for the first time, suggests the involvement of the NNMT gene rs694539 variant in the etiology of epilepsy. PMID: 26215836
    8. this study provides the first demonstration that NNMT plays a role in the resistance to 5-fluorouracil in colorectal cancer cells PMID: 27323852
    9. We suggest that lymph node metastatic adenoid cystic carcinoma (AdCC) cells acquire cancer stem cell features involving the up-regulation of NNMT and the loss of gap junction protein alpha-1, leading to epithelial-mesenchymal transition and consequent AdCC metastasis. PMID: 29277772
    10. Progression of pulmonary hypertension is associated with the activation of the NNMT-1-methylnicotinamide pathway. PMID: 27581040
    11. no SNP in NNMT is significantly associated with performance in 50-m run but rs2256292 is significantly associated with performance in 1000-m run in male Chinese college students PMID: 27900880
    12. These findings indicate that the repression of NNMT may underlie nickel-induced H3K9 dimethylation by altering the cellular SAM/SAH ratio. PMID: 28420001
    13. NNMT expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    14. The results show that a SNP (rs1941404) in NNMT is significantly associated with hyperlipidemia, and the influence of rs1941404 variation on the resting energy expenditure may be the possible mechanism for rs1941404 variation to induce hyperlipidemia. PMID: 27999813
    15. NNMT gene rs694539 variant is a genetic risk factor for migraine in women. PMID: 27726107
    16. There were no associations between MTHFR C677T, NNMT rs694539 AG/AA polymorphisms and conotruncal heart disease. PMID: 25547204
    17. Data show that nicotinamide N-methyltransferase (NNMT) and the metabolic state regulate pluripotency in embryonic stem cells (hESCs). PMID: 26571212
    18. results further reinforce a central role for NNMT in the regulation of energy homeostasis and provide further mechanistic insight into the consequences of enhanced NNMT expression PMID: 26456643
    19. White adipose tissue NNMT expression is regulated in human insulin resistance and type 2 diabetes and that plasma MNA correlates with increased tissue NNMT expression and the degree of insulin resistance. PMID: 25596852
    20. The downregulation of NNMT significantly reduced in vitro tumorigenicity of A549 cells. PMID: 25204218
    21. increasing Nnmt expression or MNAM levels stabilizes sirtuin 1 protein, an effect that is required for their metabolic benefits PMID: 26168293
    22. Data sugguest that NNMT plays an important role in PANC-1 cell proliferation, metastatic potential and survival under metabolic stress. PMID: 25592232
    23. results suggest that the NNMT SNP rs694539 may have a role in the etiology of schizophrenia in a Han Chinese female population PMID: 25317069
    24. NNMT is associated with microRNA-1291-altered pancreatic carcinoma cell metabolome and suppressed tumorigenesis. PMID: 25115443
    25. NNMT enhances the capacity of tumorigenesis associated with the inhibition of cell apoptosis and the promotion of cell cycle progression in human colorectal cancer cells PMID: 25201588
    26. Down-regulation of NNMT induces apoptosis via the mitochondria-mediated pathway in breast cancer cells. PMID: 24558488
    27. The rs694539 variant of NNMT gene is a genetic risk factor for developing nonalcoholic steatohepatitis. PMID: 23964925
    28. NNMT expression regulates neurone morphology in vitro via the sequential activation of the EFNB2 and Akt cellular signalling pathways. PMID: 23764850
    29. Data indicate that nicotinamide N-methyltransferase (NNMT) positively correlated with protein kinase B (pAkt) expression and was independent adverse prognosticators of patient survival. PMID: 23838801
    30. Genetic association of the rs694539 variant of nicotinamide-N-methyltransferase gene with bipolar disorder. PMID: 24004542
    31. The nicotinamide N-methyltransferase expression levels were significantly higher in patients with bladder tumor compared to controls that showed very low or undetectable amounts of NNMT transcript and protein. PMID: 23097023
    32. High serum NNMT is associated with kidney cancer. PMID: 23479363
    33. using metabolomics, observation that NNMT impairs the methylation potential of cancer cells by consuming methyl units from S-adenosyl methionine to create the stable metabolic product 1-methylnicotinamide PMID: 23455543
    34. This study suggested that NNMT is involved in the aetiology of schizophrenia PMID: 21791160
    35. a marked increase in enzyme activity in oral cancer PMID: 22628313
    36. Structural basis of substrate recognition in human nicotinamide N-methyltransferase PMID: 21823666
    37. NNMT has a crucial role in cellular invasion via activating PI3K/Akt/SP1/MMP-2 pathway in clear cell renal cell carcinoma (ccRCC). PMID: 21045016
    38. NNMT is over-expressed in a large proportion in renal cell cancers. High NNMT expression is significantly associated with unfavorable prognosis. PMID: 20104648
    39. the present study suggests that NNMT may have potential as a biomarker and as a therapeutic target for OSCCoral squamous cell carcinoma PMID: 19924637
    40. A potential role in predicting response to radiation in bladder cancer PMID: 12216074
    41. HNF-1beta functions as a transcription activator for NNMT gene expression in some papillary thyroid cancer cells PMID: 15486044
    42. A genomewide exploration suggested NNMT as a new candidate and major determinant of plasma homocystein levels. PMID: 15849667
    43. NNMT serum levels may have significance in the early detection and in the management of patients with colorectal cancer. PMID: 16166432
    44. depsipeptide represses NNMT and HNF-1beta gene expression in some papillary thyroid cancer cells PMID: 16676400
    45. NNMT genotype is not a strong determinant of the tHcy concentration but it may have a modifying effect on plasma homocysteine concentration in Japanese men PMID: 17434578
    46. NNMT is a novel Stat3-regulated gene and may be a potential candidate for a tumor marker of various kinds of cancers PMID: 17922140
    47. No association was found between infant nicotinamide N-methyl transferase gene variants and risk for spina bifida in our study population. PMID: 18553462
    48. Adipose tissue ss a source of nicotinamide N-methyltransferase and homocysteine PMID: 18996527
    49. For the first time, we associate the RFC1 80G>A and NNMT IVS -151C>T variants to an increased acute lymphoblastic leukemia susceptibility. PMID: 19020309
    50. NNMT gene expression is associated with tumor stage and DFS time in hepatocellular carcinoma cases. PMID: 19216803

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, NNMT/PNMT/TEMT family
  • 组织特异性：
    Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.
  • 数据库链接：

    HGNC: 7861

    OMIM: 600008

    KEGG: hsa:4837

    STRING: 9606.ENSP00000299964

    UniGene: Hs.503911