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  4. Recombinant Human Nexilin (NEXN)

Recombinant Human Nexilin (NEXN)

  • 货号：
    CSB-YP605897HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP605897HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP605897HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP605897HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP605897HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NEXN
  • Uniprot No.：
  • 别名：
    1110046H09Rik; AA553326; F-actin binding protein ; F-actin-binding protein; MGC104234; MGC138865; MGC138866; Nelin; Nexilin; Nexn; NEXN_HUMAN; nF actin binding protein; OTTHUMP00000038336
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-675
  • 氨基酸序列
    MNDISQKAEI LLSSSKPVPK TYVPKLGKGD VKDKFEAMQR AREERNQRRS RDEKQRRKEQ YIREREWNRR KQEIKEMLAS DDEEDVSSKV EKAYVPKLTG TVKGRFAEME KQRQEEQRKR TEEERKRRIE QDMLEKRKIQ RELAKRAEQI EDINNTGTES ASEEGDDSLL ITVVPVKSYK TSGKMKKNFE DLEKEREEKE RIKYEEDKRI RYEEQRPSLK EAKCLSLVMD DEIESEAKKE SLSPGKLKLT FEELERQRQE NRKKQAEEEA RKRLEEEKRA FEEARRQMVN EDEENQDTAK IFKGYRPGKL KLSFEEMERQ RREDEKRKAE EEARRRIEEE KKAFAEARRN MVVDDDSPEM YKTISQEFLT PGKLEINFEE LLKQKMEEEK RRTEEERKHK LEMEKQEFEQ LRQEMGEEEE ENETFGLSRE YEELIKLKRS GSIQAKNLKS KFEKIGQLSE KEIQKKIEEE RARRRAIDLE IKEREAENFH EEDDVDVRPA RKSEAPFTHK VNMKARFEQM AKAREEEEQR RIEEQKLLRM QFEQREIDAA LQKKREEEEE EEGSIMNGST AEDEEQTRSG APWFKKPLKN TSVVDSEPVR FTVKVTGEPK PEITWWFEGE ILQDGEDYQY IERGETYCLY LPETFPEDGG EYMCKAVNNK GSAASTCILT IESKN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：
    人Nexilin（Nexn）是一种参与肌肉收缩的蛋白质，在肌肉细胞的运动过程中发挥着重要的作用。如果想要重组这种蛋白，可以按照以下步骤进行：
    克隆Nexn基因：可以使用PCR技术从人的基因组DNA中扩增Nexn基因的开放阅读框（ORF）序列。此外，还需要添加一些启动子和标签序列，以便在表达过程中调节基因表达，并且可以方便地检测到蛋白质。
    构建表达载体：将Nexn基因插入表达载体中，可以选择一些经过验证的表达载体，如pET系列载体。这些载体中包含了一些促进蛋白表达的元件，如启动子、增强子、终止子等。
    选择表达宿主细胞：可以选择一些常用的表达宿主细胞，如哺乳动物细胞，例如CHO细胞、HEK293细胞等，并在细胞中转染含有Nexn基因的表达载体。这些宿主细胞中还需要添加一些辅助蛋白质，如转染脂质、抗生素等，以帮助蛋白质的正确折叠和纯化。
    蛋白表达和纯化：在表达宿主细胞中诱导Nexn蛋白的表达，并通过细胞破碎和离心等方法提取蛋白质。然后使用亲和层析、离子交换层析、凝胶过滤层析等方法对蛋白进行纯化。
    蛋白质的鉴定和活性检测：使用SDS-PAGE、Western blot等方法对纯化后的Nexn蛋白进行鉴定和定量。此外，还可以使用细胞实验等方法对Nexn蛋白的生物活性进行检测，如检测其对肌肉细胞的收缩和运动的影响。
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in regulating cell migration through association with the actin cytoskeleton. Has an essential role in the maintenance of Z line and sarcomere integrity.
  • 基因功能参考文献：
    1. NELINinduced phenotypic transformation of human vascular smooth muscle cell was regulated via the RhoA/SRF signaling pathway. PMID: 26458985
    2. NEXN as a novel gene for ASD and its function to inhibit GATA4 established a critical regulation of an F-actin binding protein on a transcription factor in cardiac development PMID: 24866383
    3. This is the first study to identify NEXN as a novel coronary artery disease susceptibility gene with both genetic and functional evidence. PMID: 24349201
    4. Nexilin is a component of the machinery that drives the formation of Listeria monocytogenes comet tails and enteropathogenic Escherichia coli pedestals. PMID: 22381134
    5. the mutations in NEXN that we describe here may further expand the knowledge of Z-disc genes in the pathogenesis of HCM. PMID: 20970104
    6. NELIN product is an F-actin associated protein and mediates cell motility PMID: 15823560
    7. The findings of thi study on the role of nexilin in maintaining cardiac Z-disk integrity and on the workload dependence to cardiac Z-disk damage in nexilin-linked cardiomyopathy also might have implications for the treatment of NEXN-mutation carriers. PMID: 19881492

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  • 相关疾病：
    Cardiomyopathy, dilated 1CC (CMD1CC); Cardiomyopathy, familial hypertrophic 20 (CMH20)
  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cytoplasm, myofibril, sarcomere, Z line.
  • 组织特异性：
    Abundantly expressed in heart and skeletal muscle, and at lower levels in placenta, lung, liver and pancreas. Also expressed in HeLaS3 and MOLT-4 cell lines.
  • 数据库链接：

    HGNC: 29557

    OMIM: 613121

    KEGG: hsa:91624

    STRING: 9606.ENSP00000333938

    UniGene: Hs.612385