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  4. Recombinant Human Neutrophil cytosol factor 4 (NCF4)

Recombinant Human Neutrophil cytosol factor 4 (NCF4)

  • 货号：
    CSB-YP617999HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP617999HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP617999HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP617999HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP617999HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CGD3; MGC3810; NCF 4; NCF; NCF-4; Ncf4; NCF4_HUMAN; Neutrophil cytosol factor 4; Neutrophil cytosolic factor 4; Neutrophil NADPH oxidase factor 4; p40-phox; p40phox; SH3 and PX domain-containing protein 4; SH3PXD4
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-339
  • 氨基酸序列
    MAVAQQLRAE SDFEQLPDDV AISANIADIE EKRGFTSHFV FVIEVKTKGG SKYLIYRRYR QFHALQSKLE ERFGPDSKSS ALACTLPTLP AKVYVGVKQE IAEMRIPALN AYMKSLLSLP VWVLMDEDVR IFFYQSPYDS EQVPQALRRL RPRTRKVKSV SPQGNSVDRM AAPRAEALFD FTGNSKLELN FKAGDVIFLL SRINKDWLEG TVRGATGIFP LSFVKILKDF PEEDDPTNWL RCYYYEDTIS TIKDIAVEED LSSTPLLKDL LELTRREFQR EDIALNYRDA EGDLVRLLSD EDVALMVRQA RGLPSQKRLF PWKLHITQKD NYRVYNTMP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
  • 基因功能参考文献：
    1. NCF4 polymorphism was associated with Crohn's disease, but not ulcerative colitis in Caucasian--{REVIEW} PMID: 26289093
    2. NCF4 may induce ex- pression of NADPH oxidase enzymes, such as p67phox, p47phox, p22phox and NOX2, which lead to increased ROS levels. PMID: 24378533
    3. Germline variation in NCF4, an innate immunity gene, is associated with an increased risk of colorectal cancer. PMID: 23982929
    4. The contribution of the functionally relevant NADPH polymorphisms rs1883112 and rs4673 to anthracycline-related heart lesions provides a plausible explanation for their modulation of cardiotoxicity. PMID: 23576480
    5. Constitutive and inducible intracellular production of reactive oxygen species (ROS) is higher in B cells expressing functional p40phox, supporting a direct role for p40phox in regulating B cell intracellular ROS generation. PMID: 22984083
    6. Genome-wide association studies-reported associations between the NELL1, NCF4, and FAM92B genes and susceptibility to Crohn's disease could not be replicated in Canadian children and young adults. PMID: 21472827
    7. Younger age at diagnosis, complicated disease behavior, and ileal disease location are risk factors for perianal CD. First report of an association of the NCF4 gene with perianal disease. PMID: 22158027
    8. cooperation of p40(phox) with p47(phox) for Nox2-based NADPH oxidase activation during Fcgamma receptor (FcgammaR)-mediated phagocytosis PMID: 21956105
    9. Results demonstrate that PBEF can prime for PMN respiratory burst activity by promoting p40 and p47 translocation to the membrane. PMID: 21518975
    10. no association between SNP rs4821544 and the presence of granulomas in Crohn's disease PMID: 21122541
    11. cytosolic localisation depends on direct interaction with F-actin PMID: 20637895
    12. All mutations and some polymorphisms identified in the NCF4 gene in the autosomal forms of chronic granulomatous disease are listed. Review. PMID: 20167518
    13. p40(phox) functions primarily to regulate Fc gamma receptor-induced NADPH oxidase activity rather than assembly, and stimulates superoxide production via a phosphatidylinositol-3-phosphate signal that increases after phagosome internalization. PMID: 18711001
    14. Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils PMID: 11903052
    15. Multiple PU.1 sites cooperate in the regulation of p40(phox) transcription during granulocytic differentiation of myeloid cells. PMID: 12036891
    16. p40phox and p47phox PX domains interact with PI-containing membranes PMID: 12556460
    17. A model is proposed in which phosphorylation of p40PHOX on threonine 154 leads to an inhibitory conformation that shifts the balance toward an inhibitory role and blocks NADPH oxidase activation. PMID: 15035643
    18. review of p40phox role in NADPH oxidase dynamics and possible non-NADPH oxidase processes in phagocytic and non-phagocytic cells PMID: 16102984
    19. This study identifies a role for p40(phox) and PI(3)P in coupling FcgammaR-mediated phagocytosis to activation of the NADPH oxidase. PMID: 16880255
    20. analysis of the dual regulatory mechanism through the PX domain of p40(phox): its interaction with the actin cytoskeleton may stabilize NADPH oxidase in resting cells, and binding of PtdIns (3)P potentiates superoxide production upon agonist stimulation PMID: 17698849
    21. In Swedish men with rheumatoid arthritis there were several single nucleotide polymorphisms identified in NCF4. PMID: 17897462
    22. This study has confirmed NCF4 and IRGM are risk factors for ileal Crohn's disease in New Zealand Caucasians. PMID: 18580884
    23. Class III PI3K Vps34 is responsible for the synthesis of PtdIns(3)P on phagosomes containing either S aureus or E coli. PtdIns(3)P binding to p40(phox) is important for CD18-dependent activation oxidase activation in response to S aureus and E coli PMID: 18755982
    24. regulates activity of NADPH oxidase which generates superoxide production in neutrophils. (review) PMID: 18807499
    25. p40(phox) binding to PtdIns(3)P is essential for phagocytosis-induced oxidant production in human neutrophils and its absence can be associated with disease. PMID: 19692703

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  • 相关疾病：
    Granulomatous disease, chronic, cytochrome-b-positive 3, autosomal recessive (CGD3)
  • 亚细胞定位：
    Cytoplasm, cytosol. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Membrane; Peripheral membrane protein.
  • 组织特异性：
    Expression is restricted to hematopoietic cells.
  • 数据库链接：

    HGNC: 7662

    OMIM: 601488

    KEGG: hsa:4689

    STRING: 9606.ENSP00000380334

    UniGene: Hs.474781