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  4. Recombinant Human Neutrophil cytosol factor 1 (NCF1)

Recombinant Human Neutrophil cytosol factor 1 (NCF1)

  • 货号：
    CSB-YP015525HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015525HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015525HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015525HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015525HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF 47K; NCF-1; NCF-47K; Ncf1; NCF1_HUMAN; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox organizing protein 2; Nox-organizing protein 2; NOXO2; p47 phox; p47-phox; SH3 and PX domain containing protein 1A; SH3 and PX domain-containing protein 1A; SH3PXD1A
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-390
  • 氨基酸序列
    MGDTFIRHIA LLGFEKRFVP SQHYVYMFLV KWQDLSEKVV YRRFTEIYEF HKTLKEMFPI EAGAINPENR IIPHLPAPKW FDGQRAAENR QGTLTEYCGT LMSLPTKISR CPHLLDFFKV RPDDLKLPTD NQTKKPETYL MPKDGKSTAT DITGPIILQT YRAIANYEKT SGSEMALSTG DVVEVVEKSE SGWWFCQMKA KRGWIPASFL EPLDSPDETE DPEPNYAGEP YVAIKAYTAV EGDEVSLLEG EAVEVIHKLL DGWWVIRKDD VTGYFPSMYL QKSGQDVSQA QRQIKRGAPP RRSSIRNAHS IHQRSRKRLS QDAYRRNSVR FLQQRRRQAR PGPQSPGSPL EEERQTQRSK PQPAVPPRPS ADLILNRCSE STKRKLASAV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • 基因功能参考文献：
    1. IL-27 enhances the potential of reactive oxygen species generation from monocyte-derived macrophages and dendritic cells by induction of p47(phox). PMID: 28240310
    2. p47phox, but not p67phox or p40phox, binds to and activates Nrf2, enhancing the function of Nrf2 in suppressing inflammation. PMID: 28939422
    3. p47phox S-glutathionylation plays an essential key role in the sustained ROS generation by human neutrophils. PMID: 29195919
    4. patients with hereditary p47phox deficiency show reduced platelet activation suggesting a role for this Nox cytosolic subunit in platelet activation. PMID: 27723093
    5. Decreased and increased copy numbers of NCF1 predispose to and protect against SLE. PMID: 28135245
    6. Lysophosphatidylcholines prime polymorphonuclear neutrophil through Hck-dependent activation of PKCdelta, which stimulates PKCgamma, resulting in translocation of phosphorylated p47(phox). PMID: 27531930
    7. There was an increased frequency of the NCF1-339 T allele in patients with systemic lupus erythematosus. The NCF1-339 T allele reduced extracellular ROS production in neutrophils and led to an increase expression of type 1 interferon-regulated genes. PMID: 28606963
    8. Skeletal muscle protein expression of the NADPH oxidase subunits p22(phox), p47(phox), and p67(phox) was increased in obese relative to lean subjects, where p22(phox) and p67(phox) expression was attenuated by exercise training in obese subjects. PMID: 27765769
    9. A novel role for Spns2 and S1P1&2 in the activation of p47(phox) and production of reactive oxygen species involved in hyperoxia-mediated lung injury. PMID: 27343196
    10. Study provides evidence for a novel PKC-zeta to p47phox interaction that is required for cell transformation from blebbishields and ROS production in cancer cells. PMID: 27040869
    11. Overexpression of p47phox is associated with increased migration/metastasis rate in melanoma. PMID: 26760964
    12. A rare mutation in NCF1 encoding p47phox of the leukocyte NADPH oxidase causes lack of superoxide generation, leads to chronic granulomatous disease and was recently (1200-2300 years ago) introduced into the Kavkazi Jewish population PMID: 26460255
    13. Data show that diphenylene iodonium (DPI) and apocynin can reduce hyperoxia-induced reactive oxygen species (ROS) production by decreasing the translocation and level of NADPH Oxidase p47phox. PMID: 26728380
    14. increased levels of gp91phox, p47phox and p22phox likely account for the interferon-gamma mediated enhancement of dimethyl sulfoxide-induced Nox2 activity. PMID: 26317224
    15. Results identifies p47phox-dependent NADPH oxidase activity as a critical component of Angpt-1-mediated endothelial barrier defense against classic inflammatory permeability factors. PMID: 25761062
    16. DCLRE1C and NCF1 mutations have been found by whole-genome sequencing to cause primary immunodeficiency in unrelated patients. PMID: 25981738
    17. TLR8, but not TLR7, is involved in priming of human neutrophil reactive oxygen species production by inducing the phosphorylation of p47phox and p38 MAPK. PMID: 25877926
    18. Reduced carotid but not coronary artery atherosclerosis in patients with chronic granulomatous disease despite the high prevalence of traditional risk factors raises questions about the role of NADPH oxidase in the pathogenesis of atherosclerosis. PMID: 25239440
    19. Four novel mutations in the NCF1, NCF2, and CYBB genees have been identified in chronic granulomatous disease patients in Morocco. PMID: 24596025
    20. Suggest eupafolin attenuated COX-2 expression leading to reduced production of prostaglandin E2 by blocking Nox2/p47(phox) pathway. PMID: 24967690
    21. p47(phox) and Rac2 accumulate only transiently at the phagosome at the onset of NADPH activity and detach from the phagosome before the end of reactive oxygen species production. PMID: 23870057
    22. There was an increase in p47-phox phosphorylation in neutrophils from myeloproliferative disorder patients with the JAK2 (V617F) mutation. PMID: 23975181
    23. Two novel mutations are identified in Greek patients with chronic granulomatous disease: one in NCF1 and one in cytochrome CYBB. PMID: 24081483
    24. Williams syndrome patients are at risk for increased aortic stiffness. This vascular stiffness is caused by elastin insufficiency and is modified by NCF1 copy number. PMID: 24126171
    25. Data show that curcumin-loaded polyvinylpyrrolidone nanoparticles (CURN) decreased the expression of ICAM-1, inhibited NADPH oxidase (NOX)-derived ROS generation, and reduced MAPKs and AP-1 transcription factor binding activities. PMID: 23671702
    26. this study identified a 10 % incidence of diabetes in p47 (phox) deficient chronic granulomatous disease (CGD), but none in X-linked CGD. PMID: 23386289
    27. three different cross-over points exist within the NCF1 gene cluster, indicating that autosomal p47(phox)-deficient CGD is genetically heterogeneous but can be dissected in detail by MLPA PMID: 23688784
    28. Patients with p47(phox) hereditary deficiency have intermediate flow mediated dilation and oxidative stress compared to healthy subjects and patients with NOX2 deficiency. PMID: 23216310
    29. Defining p47-phox deficient Chronic Granulomatous Disease in a Malay family. PMID: 23393912
    30. Resveratrol decreases hyperglycemic induced superoxide production via up-regulation of SIRT1, induction of FOXO3a and inhibition of p47phox in monocytes. PMID: 21813271
    31. There is no correlation between C923T(Ala308Val)polymorphism and cerebral hemorrhage in Han people in Hunan province. PMID: 21566280
    32. The low affinity and selectivity of the atypical phosphoinositide-binding site on the p47(phox) PX domain suggest that different types of phosphoinositides sequentially bind to the p47(phox) PX domain PMID: 22493288
    33. Phosphorylation of p47(phox) at different serine sites plays distinct roles in endothelial cell response to TNFalpha stimulation. PMID: 22460559
    34. A diffuse cytosolic distribution of p47-phox was observed in neutrophils from HIV-infected patients. PMID: 22690528
    35. MLCK is essential for the translocation and association of cortactin and p47phox. PMID: 22219181
    36. an increased copy number of NCF1 can be protective against developing RA and add support to previous findings of a role of NCF1 and the phagocyte NADPH oxidase complex in RA pathogenesis. PMID: 21728841
    37. autosomal recessive mutational defects are the predominant subtype in Iranian patients with chronic granulomatous disease PMID: 21789723
    38. cooperation of p40(phox) with p47(phox) for Nox2-based NADPH oxidase activation during Fcgamma receptor (FcgammaR)-mediated phagocytosis PMID: 21956105
    39. Data implicate p47phox as one of the sources of oxidative stress in diabetic islets or beta cells during hyperglycemia; evidence supports accelerated Rac1-Nox-ROS-JNK1/2 signaling pathway leading to mitochondrial dysregulation. PMID: 21911753
    40. tein disulfide isomerase redox-dependent association with p47(phox): evidence for an organizer role in leukocyte NADPH oxidase activation. PMID: 21791598
    41. Results demonstrate that PBEF can prime for PMN respiratory burst activity by promoting p40 and p47 translocation to the membrane. PMID: 21518975
    42. Direct contact of solid tumor cells and ECs activates endothelial NAD(P)H oxidase-mediated superoxide production. The oxidative stress contributes to EC apoptosis which in turn facilitates tumor cell extravasation. PMID: 21506107
    43. a differential and agonist-dependent role of the p47(phox) PX domain for neutrophil NADPH oxidase activation. PMID: 20817944
    44. granulomatous disease in Iran is predominantly due to mutations in p47-phox, while the number of mutations in p22-phox is roughly equal to that in gp91-phox, indicating that the genetics of CGD are ethnically variable PMID: 20407811
    45. Loss of p47phox is associated with inflammasome activation resulting in chronic granulomatous disease. PMID: 20495074
    46. p47phox molecular activation for assembly of the neutrophil NADPH oxidase complex. PMID: 20592030
    47. mutations in CYBB, NCF1, CYBA or NCF2 may play a role in chronic granulomatous disease PMID: 18546332
    48. There is an increased expression of NADPH oxidase p47(-PHOX) and p67(-PHOX) factor in idiopathic pulmonary fibrosis patients. PMID: 17651608
    49. All mutations and some polymorphisms identified in the NCF1 gene in the autosomal forms of chronic granulomatous disease are listed. Review. PMID: 20167518
    50. Expression of the p47phox subunit and NOX activity was evaluated in affected (superior and middle temporal gyri) and unaffected (cerebellum) brain regions from a longitudinally followed group of patients with varying degrees of cognitive impairment. PMID: 19929442

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  • 相关疾病：
    Granulomatous disease, chronic, cytochrome-b-positive 1, autosomal recessive (CGD1)
  • 亚细胞定位：
    Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side.
  • 组织特异性：
    Detected in peripheral blood monocytes and neutrophils (at protein level).
  • 数据库链接：

    HGNC: 7660

    OMIM: 233700

    KEGG: hsa:653361

    STRING: 9606.ENSP00000289473

    UniGene: Hs.647047