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  4. Recombinant Human Neuronal acetylcholine receptor subunit alpha-6 (CHRNA6), partial

Recombinant Human Neuronal acetylcholine receptor subunit alpha-6 (CHRNA6), partial

  • 货号：
    CSB-YP620896HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP620896HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP620896HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP620896HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP620896HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CHRNA6
  • Uniprot No.：
  • 别名：
    CHRNA6; Neuronal acetylcholine receptor subunit alpha-6
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • 基因功能参考文献：
    1. To gain a better understanding of the pathological processes underlying ND and ND-related behaviors and to promote the development of effective smoking cessation therapies, we here present the most recent studies concerning the genetic effects of the CHRNB3-CHRNA6 gene cluster in ND. PMID: 27327258
    2. More low frequency variants in the CHRNA6/CHRNB3 gene region were observed in cases compared to controls. PMID: 27085880
    3. Chrna6 has a cellular expression signature for retinal ganglion cells with high correlation to Thy1, a known marker. Immunofluorescence confirms this gene is preferentially expressed by RGCs in the young and adult mouse retina and expression is reduced in glaucoma. PMID: 23002780
    4. Data show efficient expression of (alpha6beta2)2beta3 nicotinic acetylcholine receptors (AChRs) in Xenopus oocytes using free subunits with only small changes in alpha6 subunits, while not altering AChR pharmacology or channel structure. PMID: 25068303
    5. the CHRNB3-A6 locus contains multiple variants affecting risk for vulnerability to cocaine and nicotine dependence as well as bipolar disorder, suggesting that they have pleiotropic effects. PMID: 24675634
    6. Polymorphism in CHRNA6 gene is associated with esophageal adenocarcinoma. PMID: 25823894
    7. The common variant rs13273442 in the CHRNB3-CHNRA6 region is associated significantly with nicotine dependence in European Americans and African Americans. PMID: 24401102
    8. N-terminal alpha-helix (Asp57); complementary face/inner beta-fold (Arg87 or Asp92) and principal face/outer beta-fold (Ser156 or Asn171) residues in the halpha6 subunit are crucial for functional expression. PMID: 24886653
    9. these results demonstrate that the combined effect of rs6474412-C/T polymorphism in smoking-related CHRNB3-CHRNA6 region gene and smoking behavior may not confer risk to psoriasis vulgaris (PV), but may have impact on PV severity in Chinese Han population. PMID: 24792900
    10. findings indicate that both CHRNA2 and CHRNA6 play a significant role in the etiology of ND in AA and EA smokers PMID: 24253422
    11. Results demonstrate the association between SNPs in CHRNA6 nicotinic acetylcholine receptor genes and the risk of smoking in ADHD PMID: 23899432
    12. Elucidation of molecular impediments in the alpha6 subunit for in vitro expression of functional alpha6beta4* nicotinic acetylcholine receptors. PMID: 24085295
    13. Cross sectional data of US adults from the NHANES linked with genotype and geocodes were used to identify tobacco use phenotypes, state-level taxation rates, and variation in the nicotinic acetylcholine receptor (CHRNA6) genotype PMID: 23227187
    14. beta3 subunit coexpression promotes function of alpha6*-nAChR PMID: 22315221
    15. alpha6beta4* nAChRs are expressed and contribute to exocytosis in human chromaffin cells of the adrenal gland, the main source of adrenaline under stressful situations. PMID: 21917987
    16. analysis of how nicotinic acetylcholine receptor (nAChR) alpha5 subunits and/or variants modulate function of alpha6*-nAChR PMID: 21873428
    17. analysis of N-terminal extracellular domain determinants in nicotinic acetylcholine receptor (nAChR) alpha6 subunits that influence effects of wild-type or mutant beta3 subunits on function of alpha6beta2*- or alpha6beta4*-nAChR PMID: 21832048
    18. CHRNB3 and CHRNA6 polymorphisms are associated with smoking behavior and lung cancer susceptibility in Chinese Han population. PMID: 21831805
    19. Three single nucleotide polymorphisms (SNPs) in CHRNA6 and one SNP in CHRNB3 are associated with a composite of alcohol phenotypes. PMID: 19500157
    20. Concatameric pentamers and pentamers formed from combinations of trimers, dimers, and monomers of alpha6beta2beta3* acetylcholine receptors exhibit similar properties, indicating that the linkers between subunits do not alter their functional properties. PMID: 20923852
    21. The negative consequences of Prenatal exposure to maternal cigarette smoking on the brain and behavior of adolescence and suggestion that the alpha6 nAChR subunit may modify, at least in part, these effects. PMID: 20029407
    22. CHRNB3-CHRNA6 and CYP2A6 sequence variants affect smoking behavior PMID: 20418888
    23. present findings disclose a haplotype association between the CHRNA6 gene and heavy alcohol use as well as an association of the CHRNA4 gene with increased body mass in heavy consumers of alcohol PMID: 19698703
    24. CHRNA6 is associated with subjective responses to tobacco. PMID: 18055561
    25. Together these results further implicate the region downstream of CHRNA6 and the region upstream of CHRNB3 in risk of nicotine dependence. PMID: 18704094
    26. Alpha6 subunit-containing nicotinic receptors play a selective role in the modulation of dopamine release in both the nucleus accumbens and in the dorsal striatum. PMID: 18940582
    27. Activating transgenic gain-of-function alpha 6 nicotinic receptors in dopamine neurons is sufficient to cause locomotor hyperactivity. PMID: 18940593

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Acetylcholine receptor (TC 1.A.9.1) subfamily, Alpha-6/CHRNA6 sub-subfamily
  • 数据库链接：

    HGNC: 15963

    OMIM: 606888

    KEGG: hsa:8973

    STRING: 9606.ENSP00000276410

    UniGene: Hs.103128