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  4. Recombinant Human Neuronal acetylcholine receptor subunit alpha-3 (CHRNA3), partial

Recombinant Human Neuronal acetylcholine receptor subunit alpha-3 (CHRNA3), partial

  • 货号：
    CSB-MP005389HU1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：
  • 货号：
    CSB-EP005389HU1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Name:CHRNA3Synonyms:NACHRA3
  • Uniprot No.：
  • 别名：
    ACHA3_HUMAN; AChR; Cholinergic receptor neuronal nicotinic alpha polypeptide 3; Cholinergic receptor nicotinic alpha 3; Cholinergic receptor nicotinic alpha polypeptide 3; CHRNA 3; CHRNA3; LNCR2; MGC104879; NACHRA 3; NACHRA3; Neuronal acetylcholine receptor protein alpha 3 chain precursor; Neuronal acetylcholine receptor subunit alpha 3; Neuronal acetylcholine receptor subunit alpha-3; Neuronal nicotinic acetylcholine receptor alpha 3 subunit; PAOD2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 表达区域：
    32-240
  • 氨基酸序列
    SEAEHRLFERLFEDYNEIIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDYGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYPDITYSLYIRRL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • 基因功能参考文献：
    1. Frequency of the cholinergic receptor nicotinic alpha 5 (CHRNA5) rs16969968-A allele and cholinergic receptor nicotinic alpha 3 (CHRNA3) rs1051730-T allele were significantly higher in lung cancer than in normal controls. PMID: 29609626
    2. Data indicate potential associations between CHRNA3polymorphisms and schizophrenia susceptibility, and the significant variants identified in our study may be used as genetic biomarkers for schizophrenia susceptibility in Chinese Han population PMID: 29879020
    3. The rs13180 (IREB2), rs16969968 (CHRNA5) and rs1051730 (CHRNA3) were significantly associated with Chronic obstructive pulmonary disease (COPD) in additive model [Padj =0.00001, odds ratio (OR)=0.64; Padj =0.0001, OR=1.41 and Padj =0.0001, OR=1.47]. The C-G haplotype by rs13180 and rs1051730 was a protective factor for COPD in our population (Padj =0.0005, OR=0.61). PMID: 28474623
    4. our results show that a genomic region with functionally related genes, such as the CHRNA5/CHRNA3/CHRNB4 cluster, is under coordinated regulatory control. PMID: 27758088
    5. CHRNA3 genetic risk score was associated with successful smoking cessation in a Chinese rural population. PMID: 28191914
    6. The minor allele increased the risk of COPD when compared to the population at large. Homozygosity for the risk allele was associated in both cohorts with all-cause mortality, with any type of cancer among the COPD patients and with the number of pack-years among the male smokers. PMID: 26751916
    7. The results of this study suggests a pleiotropic role of Chr15q25 CHRNA5-CHRNA3-CHRNB4 gene cluster with complex influences in ADHD, tobacco smoking and cognitive performance. PMID: 27302872
    8. both rs578776 and rs938682 of CHRNA3 were significantly associated with the susceptibility of lung cancer. PMID: 26831765
    9. the association of 3 selected single-nucleotide polymorphisms (CHRNA3 rs1051730, rs6495308, and CHRNA5 rs55853898) with nicotine dependence in an isolated population of Kashubians from Poland, is reported. PMID: 27127891
    10. CHRNA3 rs1051730 (G > A) and AGPHD1 rs8034191 (A > G) were more susceptible to lung cancers than noncarriers. PMID: 27072204
    11. focus on the CHRNA5/A3/B4 gene cluster and its role in nicotine dependence (review) PMID: 25471942
    12. At CHRNA3, allele G of rs3743078 was associated with increased nicotine craving. PMID: 26997181
    13. Our data suggest that gene variance in the CHRNA5-CHRNA3-CHRNB4 cluster is associated with an increased risk of death, incidence of COPD and tobacco-related cancer in smokers. PMID: 26689306
    14. two SNPs (rs6495308 and rs11072768) in CHRNA5-A3-B4 have a indirect effect on lung cancer through smoking behaviors PMID: 26942719
    15. CHRNA3 - candidate gene for Chronic Obstructive Pulmonary Disease identified by Genome-wide association studies. PMID: 26527870
    16. Data suggest that CHRNA5-A3-B4 gene variants do not exhibit a robust association with smoking cessation and are unlikely to be useful for clinically optimizing smoking cessation pharmacotherapy for Caucasian smokers. PMID: 26010901
    17. The positive interaction between heavy smoking and the homozygous mutant CHRNA3 rs6495308 genotype was found to affect the likelihood of hypertension in Chinese male smokers. PMID: 25874685
    18. Four SNPs in the CHRNA3/5 locus are associated with chronic obstructive pulmonary disease risk. [Meta-analysis] PMID: 25051068
    19. CHRNA5-A3-B4 genotype associates with body mass index in never smokers. PMID: 25474695
    20. Of the 2305 SNPs identified in the CHRNA3 gene, 115 were found to be non-synonymous and 12 and 15 nsSNPs were found to be in the 5' and 3' UTRs, respectively. 115 nsSNPs investigated, eight were predicted to be deleterious by both SIFT and PredictSNP servers. PMID: 26002565
    21. Our findings demonstrated that CHRNA3 gene rs6495309 polymorphism might be a risk factor for the development of lung cancer in Chinese. PMID: 25288178
    22. Study provides new evidence that the overexpression of the CHRNA5/A3/B4 region disrupts pyramidal neuronal structure in the hippocampus, and thus affecting the cognitive capacities PMID: 25384568
    23. the haplotype analysis found that the haplotypes "TCAC" and "CTGT," composed of rs938682, rs12914385, rs11637630, and rs2869546, were associated with a 1.79-fold and 501-fold increased lung cancer risk PMID: 25656608
    24. The frequencies of genotype and allele in CHRNA3 (rs8040868) and PHACTR2 (rs9390123) were not significantly different between the NSCLC cases and controls, or between either of the subgroups. PMID: 25399010
    25. GABRR2 and CHRNA3 were found to be differentially expressed after risperidone treatment. These genes may be regulated by antipsychotic use. PMID: 24113126
    26. Suggest no relationship between these smoking-related SNPs in the CHRNA5/A3/B4 gene cluster and psoriasis vulgaris in Chinese Han population. PMID: 25297392
    27. In Mendelian randomisation analyses, there was no strong evidence that the minor allele of rs16969968/rs1051730 was associated with depression, anxiety or psychological distress in current or former smokers. [Meta-analysis] PMID: 25293386
    28. The alpha3beta4* nicotinic ACh receptor subtype mediates physical dependence to morphine PMID: 24750073
    29. High cumulative tobacco consumption is associated with short telomeres observationally, but there is no clear genetic association. PMID: 24906368
    30. Results show that CHRNA3 genotype is associated with decreased lung function and risk of chronic obstructive pulmonary disease. among ever-smokers, whereas this was not the case for CYP3A5*3. PMID: 24535486
    31. Increased expression of NeuroD1 subsequently leads to regulation of expression and function of the nicotinic acetylcholine receptor subunit cluster of alpha3, alpha5, and beta4. PMID: 24719457
    32. CHRNA3 polymorphism functions as a genetic modifier of the risk of developing lung ADC in the Chinese population, particularly in nonsmoking females. PMID: 24686516
    33. The minor alleles of two polymorphisms (rs578776 and rs3743078) in the CHRNA3 gene are associated with an increased risk of tobacco smoking only among patients with ADHD. PMID: 24375168
    34. Elucidation of the signaling events elicited upon agonist binding to corneal mAChRs and nAChRs will be crucial for understanding the mechanisms of ACh signaling in CECs, which has salient clinical implications. PMID: 25270189
    35. rs1051730 polymorphism may modify susceptibility to lung cancer via a smoking-independent manner among Chinese Han population. PMID: 24337855
    36. Rare missense variants in CHRNB3 and CHRNA3 are associated with risk of alcohol and cocaine dependence. PMID: 24057674
    37. Genetic variants in the CHRNA5-A3-B4 gene cluster alter nicotine intake and body mass index in a population of Alaska Native people, who have a distinct haplotype structure, smoking behaviors and prevalence of obesity. PMID: 23692359
    38. Investigated associations between neuroticism and 13 SNPs in the CHRNA5 and CHRNA3 genes in young adult Mexican American men and women. PMID: 24588897
    39. Women with the variant AA genotype of CHRNA3 rs578775 were at significantly decreased risk of heavy smoking. PMID: 21810735
    40. study identified an association between a nicotinic acetylcholine receptor a3 subunit variant (rs578776)and reward-related neural response in a large cohort of healthy non-smoking adolescents. PMID: 23689675
    41. Genetic variations in CHRNA3 are associated with COPD in the Korean population. PMID: 23207642
    42. Genetic variation in nicotinic receptor gene CHRNA3 might be an important connecting link between early attentional processes and smoking behavior. PMID: 23604333
    43. CHRNA3 gene rs3743073G variant genotype significantly increased lung cancer risk, especially in male smokers over the age of 60. PMID: 23023782
    44. Rare genetic variation in the CHRNA5-A3-B4 gene cluster contributes modestly to the level of response to alcohol. PMID: 23458267
    45. CHRNA5-A3-B4 rs667282 TT/TG genotypes were associated with significantly increased risk of esophageal squamous cell carcinoma. PMID: 23844051
    46. CHRNA3 polymorphism was associated with pack-year of smoking in chronic obstructive pulmonary disease Chinese Han patients PMID: 22914670
    47. Our findings uncover a novel mechanism of nicotine-induced alpha3beta4 nAChR upregulation that may be relevant also for other nAChR subtypes. PMID: 23884938
    48. silencing alpha3 nAChR mRNA might enhance the effect of Abeta25-35 on cell apoptosis by increasing the levels of p38 protein and bax mRNA and decreasing the level of bcl-2 mRNA, which may play a role in the pathogenesis of Alzheimer's disease PMID: 23710919
    49. SNP rs1051730 associated with smoking and body mass index PMID: 23729684
    50. we evaluate the utility of genetic variants in CHRNA3 for smoking as a risk factor in low birth weight PMID: 23701534

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  • 亚细胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Acetylcholine receptor (TC 1.A.9.1) subfamily, Alpha-3/CHRNA3 sub-subfamily
  • 数据库链接：

    HGNC: 1957

    OMIM: 118503

    KEGG: hsa:1136

    STRING: 9606.ENSP00000315602

    UniGene: Hs.89605