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  4. Recombinant Human Neuroglobin (NGB)

Recombinant Human Neuroglobin (NGB)

  • 货号：
    CSB-YP015776HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015776HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015776HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015776HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NGB
  • Uniprot No.：
  • 别名：
    Neuroglobin; NGB; NGB_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-151
  • 氨基酸序列
    MERPEPELIR QSWRAVSRSP LEHGTVLFAR LFALEPDLLP LFQYNCRQFS SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLA SLGRKHRAVG VKLSSFSTVG ESLLYMLEKC LGPAFTPATR AAWSQLYGAV VQAMSRGWDG E
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.
  • 基因功能参考文献：
    1. Neuroglobin functions as an important modulator of the Wnt/beta-Catenin and NFkappaB signaling pathway through direct interaction with Dishevelled-1. PMID: 30041403
    2. This study demonstrated that serum NGB levels were significantly associated with 6-month unfavorable outcomes, indicating that Ngb can play a role in predicting 6-month unfavorable outcomes. PMID: 29738858
    3. Results show that the expression of NGB in glioma is grade related and is negatively regulated at the transcriptional level by PPARgamma suggesting an important role of NGB in glioma progression. PMID: 28410531
    4. The s demonstrate that Glu53, Glu60, and Glu118 of human Ngb are crucial for both the neuroprotective activity and interaction with Gi. Moreover, they show that Lys46, Lys70, Arg208, Lys209, and Lys210 residues of Gi are important for binding to human Ngb. PMID: 27109834
    5. Ngb-H64Q-CCC binds CO about 500 times more strongly than hemoglobin, which could be useful in detecting and eliminating carbon monoxide poisoning PMID: 27928027
    6. these results highlight the involvement of ERa activation and of E2/ERa-dependent NGB upregulation in the insensitivity of MCF-7 to paclitaxel. These novel findings could have important implications in the development of targeted therapeutics for overcoming paclitaxel insensitivity in ERa-positive human breast cancer PMID: 27312786
    7. Knockdown of GATA-2 caused NGB expression to drop dramatically, indicating GATA-2 as an essential transcription factor for the activation of NGB expression via binding to a novel distal regulatory element of NGB. PMID: 27651453
    8. analysis of how the interaction of hydrogen sulfide with human neuroglobin is muted by a distal ligand PMID: 28246171
    9. NO oxidation kinetics at physiological NO concentrations. The use of a polarized electrode to efficiently interconvert the ferric (Fe(3+)) and ferrous (Fe(2+)) forms of an immobilized NGB showed that the disulfide bridge both defines the kinetics of NO dioxygenase activity and regulates appearance of the free ferrous deoxy-NGB, which is the redox active form of the protein in contrast to oxy-NGB. PMID: 27402851
    10. Taken together, this work support the first evidence that Ngb can be potentially used as a promising biomarker and target for novel treatment of human glioma PMID: 28103511
    11. The cochlear nuclei and superior olivary complex (SOC) also express considerable amounts of Ngb. Within the human SOC, many medial superior olive neurons, and scattered neurons in the LSO, were Ngb-immunoreactive PMID: 25636685
    12. Study characterized the presence of Ngb in the human cochlea PMID: 26556771
    13. Findings suggest a control model for neuroglobin's anti-apoptotic activity under physiological conditions. While in normoxic conditions, its activity is maintained at low level, an immediate transition to an hypoxic situation increases it drastically. PMID: 26305249
    14. The review summarizes the recent experimental findings, mainly focusing on the mechanisms of Ngb's protection and induction during ischemic/hypoxic conditions and it roles in astrocytes, tumors, as well as in neurite outgrowth. [review] PMID: 25372753
    15. Neuroglobin player role in estrogen receptor alpha-positive cancer cell viability. PMID: 25299774
    16. a mechanism for redox control of human Ngb (hNgb) activity PMID: 24855999
    17. 17beta-estradiol-induced NGB up-regulation could act as an oxidative stress sensor, which does not oppose to the pro-apoptotic 17beta-estradiol effect in ER beta-containing colon cancer cells unless a rise of oxidative stress occurs. PMID: 25683270
    18. first X-ray structure of wild-type human neuroglobin is reported at 1.74 A resolution PMID: 24699645
    19. Glu60 of human Ngb is a crucial residue for its GDI and neuroprotective activities PMID: 24376733
    20. Data show that chimeric neuroglobin and myoglobin were generated by swapping a regulatory segment. PMID: 25452214
    21. This paper for the first time presents the data on neuroglobin distribution in human cerbellum using immunohistochemistry. PMID: 25552092
    22. Findings indicate that myoglobin (Mb) and neuroglobin (Ngb) can be expressed in nonmuscle and non-neural contexts. PMID: 24446190
    23. analysis of single nucleotide polymorphisms in neuroglobin that affect transcription and possibly Alzheimer's Disease PMID: 24362753
    24. Neuroglobin brain levels are increased in Alzheimer's disease patients compared to controls. PMID: 23648513
    25. These findings suggest that neuroglobin is expressed early in the course of neuronal differentiation and may, therefore, have a role in neurodevelopment. PMID: 23643985
    26. NGB is expressed in the carotid body of heroin addicts and may have a role in compensating for the negative effects of heroin PMID: 23319379
    27. Ngb and Cygb are expressed in the solitary tract nucleus and in the carotid body, and may have roles in the processing of cardiovascular and respiratory reflex inputs PMID: 23835959
    28. Report neuroglobin expression in human brain. PMID: 23160832
    29. functional impact of heme disorder and cysteine oxidation state on the properties of the Ngb ligand PMID: 23135388
    30. the oxidative stress-induced structural changes of human Ngb are essential for its neuroprotective activity. PMID: 22787149
    31. We found that NGB was present in a rat astrocytoma cell line (C6), human astrocytoma cell line (U251), and human astrocytoma tissues PMID: 22009023
    32. overexpression in non-small cell lung cancer is associated with histological subtype, hypoxia PMID: 21601304
    33. 14-3-3 binding and phosphorylation of neuroglobin during hypoxia modulate six-to-five heme pocket coordination and rate of nitrite reduction to nitric oxide PMID: 21965683
    34. The phenylalanine B10 residue of neuroglobin plays a key role in transmitting the structural information from the disulfide bridge to the heme-pocket region. PMID: 21704585
    35. This review describes the discovery of neuroglobin over a decade ago, its structure, affinity for oxygen, and its expression in cerebral neurons, all of which suggest it plays a role in providing oxygen to the brain. PMID: 21620833
    36. Caenorhabditis elegans globin GLB-26 (expressed from gene T22C1.2) has been studied in comparison with human neuroglobin (Ngb) and cytoglobin (Cygb) for its electron transfer properties PMID: 21674044
    37. This study demonistrated that Neuroglobin overexpression in brain with arteriovenous malformation and intracerebral hemorrhage. PMID: 21725774
    38. Cimeric Myoglobin has a cell-membrane-penetrating activity similar to zebrafish Ngb. PMID: 21304818
    39. Data suggest a potential role of DNA methylation in regulating NGB tissue-specific expression. PMID: 21362510
    40. neuroglobin may function as a physiological oxidative stress sensor and a post-translationally redox-regulated nitrite reductase that generates NO under six-to-five-coordinate heme pocket control PMID: 21296891
    41. There is a plausible central role for neuroglobin in the control of apoptosis. PMID: 21190290
    42. A significant association was found between Alzheimer disease risk and a variant in the NGB gene. Data is consistent with a model where women, older individuals and carriers of specific genotypes have lower levels of NGB leading to increased risk for AD. PMID: 19010568
    43. Ngb may confer protection against ischemia-reperfusion injury in the brain through its intrinsic antioxidant properties. PMID: 20571522
    44. These data favor the hypothesis that the disulfide bond between Cys46 and Cys55 modulates the functioning of human neuroglobin. PMID: 20643048
    45. the haplotype block represented by rs3783988 in neuroglobin appears to influence recovery after severe traumatic brain injury PMID: 20345238
    46. These data provides an explanation the action of neuroglobin in the protection of nerve cells from unwanted apoptosis. PMID: 20091232
    47. The acid-induced denaturation pathway of apo-Ngb can be illustrated from the native state (N), via a partially unfolded state (U(A)) to the molten globule state (MG). PMID: 20227336
    48. Ischemic stroke increases the expresssion of the neuroprotective protein neuroglobin in brain. PMID: 20075359
    49. The conformational and functional stability of Ngb at acidic pH was analyzed. PMID: 19860834
    50. Full-length cDNA cloning and genomic organization of NGB have been reported. PMID: 11820779

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  • 亚细胞定位：
    Perikaryon. Cytoplasm. Mitochondrion.
  • 蛋白家族：
    Globin family
  • 组织特异性：
    Predominantly expressed in brain, the strongest expression is seen in the frontal lobe, the subthalamic nucleus and the thalamus.
  • 数据库链接：

    HGNC: 14077

    OMIM: 605304

    KEGG: hsa:58157

    STRING: 9606.ENSP00000298352

    UniGene: Hs.274363