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  4. Recombinant Human Neurogenic differentiation factor 1 (NEUROD1)

Recombinant Human Neurogenic differentiation factor 1 (NEUROD1)

  • 货号：
    CSB-YP615700HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP615700HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP615700HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP615700HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP615700HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    atonal; basic helix loop helix transcription factor; BETA 2; Beta cell E box transactivator 2; BETA2; BHF 1; BHF1; bHLHa3; class A basic helix loop helix protein 3; Class A basic helix-loop-helix protein 3; MODY 6; MODY6; NDF1_HUMAN; NeuroD; NeuroD1; Neurogenic differentiation 1; Neurogenic differentiation factor 1; neurogenic helix loop helix protein NEUROD; Neuronal differentiation 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-356
  • 氨基酸序列
    MTKSYSESGL MGEPQPQGPP SWTDECLSSQ DEEHEADKKE DDLETMNAEE DSLRNGGEEE DEDEDLEEEE EEEEEDDDQK PKRRGPKKKK MTKARLERFK LRRMKANARE RNRMHGLNAA LDNLRKVVPC YSKTQKLSKI ETLRLAKNYI WALSEILRSG KSPDLVSFVQ TLCKGLSQPT TNLVAGCLQL NPRTFLPEQN QDMPPHLPTA SASFPVHPYS YQSPGLPSPP YGTMDSSHVF HVKPPPHAYS AALEPFFESP LTDCTSPSFD GPLSPPLSIN GNFSFKHEPS AEFEKNYAFT MHYPAATLAG AQSHGSIFSG TAAPRCEIPI DNIMSFDSHS HHERVMSAQL NAIFHD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis.
  • 基因功能参考文献：
    1. Data suggest that the first cases of MODY6 identified in Japan are result of missense (p.L157R, c.470T>G) or frameshift mutations (p.H206PfsTer38, c.616_617insC; p.P245RfsTer17, c.734delC; p.H206TfsTer56, c.616delC) in NEUROD1. The probands and affected family members exhibit intrinsically lower capacity of insulin secretion and neurological disorders. [CASE REPORT] PMID: 28664602
    2. Data suggest the EGR1-miR-30a-5p-NEUROD1 axis might serve as a promising biomarker for diagnosis and treatment monitoring for schizophrenic patients in acute psychotic state. EGR1 and miR-30a-5p were remarkably downregulated, whereas NEUROD1 was significantly upregulated in PBMNCs from patients in acute psychotic state. PMID: 28072411
    3. Mutation in NEUROD1 gene is associated with Maturity Onset Diabetes of the Young. PMID: 28095440
    4. the importance of the oligomeric state of CtBP for coactivation of NeuroD1-dependent transcription, was investigated. PMID: 27880001
    5. NeuroD1 seemed not sufficient to induce and maintain neuronal differentiation. Induction of neuronal differentiation by overexpression of Neurog1 initiated important steps for the development of glutamatergic neurons such as the spiral ganglion neurons PMID: 27423984
    6. detected c.133A > G (p. Ala45Thr) in children with sensorineural hearing loss PMID: 26634621
    7. Study reports a family with autosomal dominant diabetes related to a new NEUROD1 mutation, one of very few meeting Maturity Onset Diabetes of the Young criteria. PMID: 26773576
    8. RNAi of lentiviral vector target NeuroD can reduce the migration and invasion abilities of PANC-1 cells PMID: 24464628
    9. this study concludes that the novel mechanism would regulate the expression of ALK in neuroblastoma and that NeuroD1 should be significantly involved in neuroblastoma tumorigenesis. PMID: 25652313
    10. NEUROD1 is important for maintenance of the retina function and partial loss-of-function mutation in NEUROD1 is likely a rare cause of nonsyndromic ARRP. PMID: 25477324
    11. Increased expression of NeuroD1 subsequently leads to regulation of expression and function of the nicotinic acetylcholine receptor subunit cluster of alpha3, alpha5, and beta4. PMID: 24719457
    12. Transactivation of Ctbp was dependent on the histone H3 lysine 9 (H3K9) demethylase activity of LSD1 facilitates subsequent H3K9 acetylation by the NeuroD1-associated histone acetyltransferase, P300/CBP-associated factor. PMID: 24732800
    13. The variant A45T does not play a major role in the development of T2 Diabetes mellitus in East Asian descent. PMID: 23203005
    14. Gene expression profiling revealed that permissive lines are typified by lower expression of the early neurogenic transcription factor ASCL1 and, conversely, by higher expression of the late neurogenic transcription factor NEUROD1. PMID: 23739064
    15. NeuroD1 regulates survival and migration of neuroendocrine lung carcinomas via signaling molecules TrkB and NCAM. PMID: 23553831
    16. The overexpression of NeuroD may contribute to the tumorogenesis and development of pancreatic carcinoma, and is closely correlated to the cancer cell proliferation, p53 signal pathway and neural invasion. PMID: 22455846
    17. Combined transfection of the three transcriptional factors, PDX-1, NeuroD1, and MafA, causes differentiation of bone marrow mesenchymal stem cells into insulin-producing cells PMID: 22761608
    18. Photosensitive photoreceptor cells can be generated by combinations of transcription factors. The combination of CRX and RX generate immature photoreceptors: and additional NEUROD promotes maturation. PMID: 22558175
    19. Most, if not all, nasal chemosensory neurons derive from NeuroD1-expressing globose basal cells of the immediate neuronal precursor variety. PMID: 21800309
    20. Findings establish the critical role of the neuronal differentiation factor NeuroD1 in neuroblastoma as well as its functional relationship with the neuronal repellent factor Slit2. PMID: 21349947
    21. ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and beta2, and activates insulin gene transcription. PMID: 21278380
    22. NeuroD alone may not be sufficient to induce regulated insulin release in insulin-producing liver cells. PMID: 21084850
    23. Human NeuroD1 under control of the cytokeratin 19 promoter can induce differerentiation of pancreatic epithelial cells into insulin producing cells. PMID: 20692411
    24. syndrome resulting from homozygous loss of function mutations in NEUROD1 which is characterized by permanent neonatal diabetes. PMID: 20573748
    25. There was no association between methylation and expression in breast tumour specimens, with only 14% exhibiting NEUROD1 expression PMID: 19353266
    26. No significant association of NEUROD1 with retinopathy or nephropathy in Croatian patients with type I diabetes PMID: 20120526
    27. regulator of insulin transcription PMID: 11755474
    28. expression during trophoblast invasion PMID: 11900979
    29. Beta-cell dysfunction in late-onset diabetic subjects carrying homozygous mutation in transcription factor NeuroD1. PMID: 12200761
    30. The genetic polymorphism in NeuroD is associated with the development of early-onset type 2 diabetes. The presence of Thr45 allele may represent a risk factor for early-onset type 2 diabetes among Chinese. PMID: 12476420
    31. Polymorphism Ala45Thr is associated with Type 1 diabetes mellitus in Czech children PMID: 12639765
    32. Ala5Thr polymorphism of NeuroD1 plays a role in the risk of NIDDM in the examined Polish population PMID: 12861411
    33. NeuroD1/E47 transcription factors up-regulate IA-1 gene expression through the proximal E-box element of the IA-1 promoter PMID: 12890672
    34. Ala45 variant of BETA2/NeuroD1 may be associated with IDDM in Caucasians. PMID: 12951629
    35. NeuroD1 is differentially expressed in pituitary adenomas and its possible ontogenetic and/or pathogenetic implications in non-corticotroph tumors are discussed. PMID: 14759067
    36. No evidence of Ala(45)Thr polymorphism of the NeueroD gene and type 1 diabetes. PMID: 15047635
    37. Focus of this review is on recent progress in understanding the important role of BETA2/NeuroD1 in initiating neuronal differentiation and maintaining the nervous system. PMID: 15247487
    38. Polymorphism contributes to glucose intolerance in a South Indian population. PMID: 15277395
    39. NeuroD controls both common and distinct sets of molecules involved in cell survival and differentiation in different tissue types [review] PMID: 15650322
    40. co-expression and functional synergism of these beta-cell enriched transactivators, MafA, Pdx1, and Beta2, are critical for establishing the beta-cell-specific and efficient expression of the insulin gene. PMID: 15993959
    41. the SREBP-1c.BETA2.E47 complex is in a DNA looping structure which is required for efficient recruitment of CREB-binding protein/p300 PMID: 16055439
    42. we demonstrated that ISL1 and BETA2 could activate insulin gene transcription synergistically. PMID: 16321656
    43. Gender-specific association of the Ala45Thr variant of NEUROD1 with Type 1 diabetes in Brazilian women. PMID: 16357810
    44. results presented in this study define INSM1 as a transcriptional repressor of the neuroD/b2 gene. The molecular mechanism of INSM1 transcriptional repression is attributed to the recruitment of cyclin D1 and HDAC-1 and -3 PMID: 16569215
    45. The NeuroD1-Ala45Thr variation may itself have an important role in susceptibility to or be in disequilibrium with early-onset T2DM in Chinese. The Ala45Thr may affect the onset pattern of T2DM, i.e., early-onset but not late-onset T2DM in Chinese. PMID: 16773428
    46. helix-loop-helix (HLH) domain of basic helix-loop-helix (bHLH) family proteins such as NeuroD facilitate protein transduction into various cell lines. PMID: 16870135
    47. Expression of NeuroD1 versus chromogranin-A is more frequent in pCA, and correlates to increased indicators of malignancy in moderately to poorly differentiated pCA. PMID: 17126478
    48. These results suggest that NeuroD plays an important role in regulated exocytosis by inducing expressions of various components required in the process. PMID: 17217914
    49. A study evaluating the extent to which common variation in the six known maturity-onset diabetes of the young (MODY) genes, which cause a monogenic form of type 2 diabetes, is associated with type 2 diabetes is presented. PMID: 17327436
    50. Mutation in the NeuroD1/BETA2 gene contributes to the development of diabetes PMID: 17440689

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  • 相关疾病：
    Maturity-onset diabetes of the young 6 (MODY6); Diabetes mellitus, non-insulin-dependent (NIDDM)
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 数据库链接：

    HGNC: 7762

    OMIM: 125853

    KEGG: hsa:4760

    STRING: 9606.ENSP00000295108

    UniGene: Hs.574626