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  4. Recombinant Human Neuroepithelial cell-transforming gene 1 protein (NET1)

Recombinant Human Neuroepithelial cell-transforming gene 1 protein (NET1)

  • 货号：
    CSB-YP768775HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP768775HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP768775HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP768775HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NET1
  • Uniprot No.：
  • 别名：
    ARHG8_HUMAN; ARHGEF8; GLYT2 ; Guanine nucleotide regulatory protein oncogene; MGC114559 ; MGC127767 ; MGC53602 ; mNET1; Net1; NET1A; Neuroepithelial cell transforming gene 1; Neuroepithelial cell-transforming gene 1 protein; p65 Net1 proto oncogene; Proto-oncogene p65 Net1; Rho guanine nucleotide exchange factor 8; Rho guanine nucleotide exchange factor GEF 8
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-596
  • 氨基酸序列
    MEPELAAQKQ PRPRRRSRRA SGLSTEGATG PSADTSGSEL DGRCSLRRGS SFTFLTPGPN WDFTLKRKRR EKDDDVVSLS SLDLKEPSNK RVRPLARVTS LANLISPVRN GAVRRFGQTI QSFTLRGDHR SPASAQKFSS RSTVPTPAKR RSSALWSEML DITMKESLTT REIRRQEAIY EMSRGEQDLI EDLKLARKAY HDPMLKLSIM SEEELTHIFG DLDSYIPLHE DLLTRIGEAT KPDGTVEQIG HILVSWLPRL NAYRGYCSNQ LAAKALLDQK KQDPRVQDFL QRCLESPFSR KLDLWSFLDI PRSRLVKYPL LLKEILKHTP KEHPDVQLLE DAILIIQGVL SDINLKKGES ECQYYIDKLE YLDEKQRDPR IEASKVLLCH GELRSKSGHK LYIFLFQDIL VLTRPVTRNE RHSYQVYRQP IPVQELVLED LQDGDVRMGG SFRGAFSNSE KAKNIFRIRF HDPSPAQSHT LQANDVFHKQ QWFNCIRAAI APFQSAGSPP ELQGLPELHE ECEGNHPSAR KLTAQRRAST VSSVTQVEVD ENAYRCGSGM QMAEDSKSLK THQTQPGIRR ARDKALSGGK RKETLV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
  • 基因功能参考文献：
    1. data suggest that NET-1 promotes HCC invasion, adhesion and growth by regulating BAX, caspase 3, caspase 8 and BCL2 expression. PMID: 30301500
    2. Study found NET1 as a direct target for miR-22 to promote chemoresistance of bladder cancer cells. PMID: 29620229
    3. We suggest that the oncogenic properties of CML cells are probably due to deregulated expression of NET1 as a result of altered expression of miR-22. PMID: 25041463
    4. Net1 promotes the angiogenesis in cervical squamous cell carcinomas and siRNA targeting of Net1 can effectively reduce angiogenesis and inhibit tumor growth via VEGF signaling. PMID: 28511963
    5. AMPK phosphorylation of the RHOA guanine nucleotide exchange factor NET1A inhibits extracellular matrix degradation, an early step in cell invasion PMID: 26456332
    6. Net1 mRNA and protein levels in non-small cell lung cancer tissues were significantly elevated compared with those in their corresponding nontumor tissues. PMID: 26459749
    7. Simultaneous silencing of NET-1 and survivin using one-chain-double-target siRNA thus provides an advantageous alternative in the development of therapeutics for skin squamous cell carcinoma . PMID: 26080853
    8. These data indicate that Net1A acetylation regulates its subcellular localization to impact on RhoA activity and actin cytoskeletal organization. PMID: 25588829
    9. NET1 was associated with comorbidity of oppositional defiant disorder and symptoms in Attention-deficit/hyperactivity disorder probands PMID: 24942521
    10. Net1A plays a plays a previously unrecognized, Rho GTPase-independent role in controlling ATM activity and downstream signaling after DNA damage to impact cell survival. PMID: 25486363
    11. High expression of neuroepithelial transforming gene 1 was associated with hepatocellular carcinoma. PMID: 25113254
    12. The overexpression of NET-1 in tumor cells may be closely related to the malignant phenotype of skin squamous cell carcinoma including proliferation, invasion and tumor growth. PMID: 24196235
    13. NET1 expression is elevated in OAC and its pre-malignant phenotype, Barrett's oesophagus. NET1 promotes OAC cell invasion and proliferation. PMID: 23945136
    14. NET-1 and VEGF silencing play a key role in inhibiting hepatocellular cell proliferation, promoting apoptosis, and reducing angiogenesis. PMID: 23636606
    15. Results identify Net1 as a novel regulator of mitosis and indicate that altered expression of Net1, as occurs in human cancers, may adversely affect genomic stability. PMID: 23864709
    16. Data suggests that NET1 may be of prognostic significance in the clinical management of gastric adenocarcinoma PMID: 24375299
    17. A combination of NET1 (rs2242447) and SNAP-25 (rs3746544) is a risk factor for ADHD. PMID: 23872233
    18. A previously unrecognized role for the Net1A isoform in controlling FAK activation during planar cell movement. PMID: 23689132
    19. Rac1 activation causes relocalization of Net1 isoforms outside the nucleus and stimulates Net1A catalytic activity. PMID: 23184663
    20. Net -1 frameshifting on a noncanonical sequence in a herpes simplex virus drug-resistant mutant is stimulated by nonstop mRNA. PMID: 22927407
    21. Net1 is a CARMA-interacting molecule and brings important information on the molecular mechanisms that control NF-kappaB transcriptional activity PMID: 22343628
    22. A mechanism involving the nuclear GEFs Ect2 and Net1 for activating RhoB after genotoxic stress, thereby facilitating cell death after treatment with DNA damaging agents. PMID: 21373644
    23. Existence of a functional Net1/RhoA signaling pathway within the nucleus of the cell and this implicates them in the DNA damage response. PMID: 21390328
    24. NET1 plays an role in gastric cancer cell migration and invasion. The gene expression profile further elucidates the molecular mechanisms underpinning NET1-mediated aggressive GC cell behaviour. PMID: 21284875
    25. RNAi targeting NET-1 gene effectively down-regulates the expression of NET-1 in A431 cells, leading to an inhibition of proliferation, migration and infiltration. PMID: 20078975
    26. Smad3 regulates RhoA activation and cytoskeletal reorganization by controlling NET1 in TGF-beta1-induced ARPE-19 cells. PMID: 20547485
    27. NET-1 protein is expressed in cytoplasm of HCC cells as irregular granules near Golgi apparatus, and may promote the uncontrolled proliferation and abnormal differentiation in HCC cells. PMID: 18478931
    28. NET1 has roles in stimulating cytoskeletal reorganization and in transforming cells PMID: 15611121
    29. PAK1 negatively regulates the activity of NET1 PMID: 15684429
    30. The putative role for NET1 genes may provide important targets for intervention in Gastric adenocarcinoma, evidenced by their role in promoting invasion and proliferation, key phenotypic hallmarks of cancer cells. PMID: 16552434
    31. These results suggest that the interaction between Net1 and Dlg1 may contribute to the mechanism of Net1-mediated transformation. PMID: 17938206
    32. study concludes expression of NET-1 may relate to proliferation, metastasis & clinic stages of hepatocellular carcinoma (HCC); expression in HCC tissues may positively correlate to TMN stages; expression in HCC tissues was positively correlated with PCNA PMID: 18214716
    33. data highlight the importance of Net1 in controlling RhoA and p38 MAPK mediated cell survival in cells exposed to DNA damaging agents PMID: 18509476
    34. the importance of NET1 as a driver of tumour cell invasion PMID: 18827818
    35. NET1 and integrin alpha6beta4 coexpression is independently associated with the development of distant metastasis in breast cancer. PMID: 19124484
    36. Net1 requires interaction with PDZ domain proteins, such as Dlg1, to protect it from proteasome-mediated degradation and to maximally stimulate RhoA and that this interaction is regulated by cell-cell contact. PMID: 19586902

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 14592

    OMIM: 606450

    KEGG: hsa:10276

    STRING: 9606.ENSP00000347134

    UniGene: Hs.25155