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  4. Recombinant Human Netrin-1 (NTN1)

Recombinant Human Netrin-1 (NTN1)

  • 货号：
    CSB-YP016127HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016127HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016127HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016127HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016127HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    epididymis tissue protein Li 131P; NET1_HUMAN; Netrin 1, mouse, homolog of; Netrin 1-like; Netrin-1; Netrin1; Ntn 1; NTN 1L; NTN1; NTN1L; Unc6
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-604
  • 氨基酸序列
    GPGLSM FAGQAAQPDP CSDENGHPRR CIPDFVNAAF GKDVRVSSTC GRPPARYCVV SERGEERLRS CHLCNASDPK KAHPPAFLTD LNNPHNLTCW QSENYLQFPH NVTLTLSLGK KFEVTYVSLQ FCSPRPESMA IYKSMDYGRT WVPFQFYSTQ CRKMYNRPHR APITKQNEQE AVCTDSHTDM RPLSGGLIAF STLDGRPSAH DFDNSPVLQD WVTATDIRVA FSRLHTFGDE NEDDSELARD SYFYAVSDLQ VGGRCKCNGH AARCVRDRDD SLVCDCRHNT AGPECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS GRKSGGVCLN CRHNTAGRHC HYCKEGYYRD MGKPITHRKA CKACDCHPVG AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSVEEPE DCDSYCKASK GKLKINMKKY CKKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR DIACKCPKIK PLKKYLLLGN AEDSPDQSGI VADKSSLVIQ WRDTWARRLR KFQQREKKGK CKKA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Involved in dorsal root ganglion axon projection towards the spinal cord. It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.
  • 基因功能参考文献：
    1. High NTN1 expression is associated with gastric cancer. PMID: 30106432
    2. increased concentrations of urinary SEMA-3A and urinary Netrin-1 are found in urine from children with severe hydronephrosis and that their concentrations are related to the degree of obstruction. PMID: 29199452
    3. miR214 reduces chemoresistance by targeting netrin1 in bladder cancer cell lines. PMID: 29328435
    4. Netrin-1 decreased in experimental autoimmune encephalomyelitis and in multiple sclerosis patients, mainly during relapse, suggesting an anti-inflammatory role of netrin-1 PMID: 28677863
    5. Such data indicate that cooperation between the UPR and UNC5A depletion as previously observed by ourselves in HCC patients samples may foster liver cancer development and growth. PMID: 29277614
    6. Netrin-1 restores cell injury and impaired angiogenesis in vascular endothelial cells upon high glucose via PI3K/AKT-eNOS signaling. PMID: 28250059
    7. Collectively, our results defined netrin-1 as a positive regulator of malignant tumor metastasis in GC by activating the YAP signaling, with potential implications for new approaches to GC therapy. PMID: 29305865
    8. Results demonstrate that NTN1 is an important regulator of stemness and motility of glioblastoma cells. Furthermore, it deciphers a novel mechanism where NTN1 activates Notch signaling and subsequent stemness in invasive glioblastoma cells. PMID: 28069038
    9. Three mutations in exon 7 of NTN1 in 2 unrelated families and 1 sporadic case with isolated congenital mirror movements (CMM), a disorder characterized by involuntary movements of one hand that mirror intentional movements of the opposite hand. PMID: 28945198
    10. Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton's jelly mesenchymal stem cells. PMID: 28241866
    11. Our study has shown an increase in netrin-1 expression that could be linked with macrophage infiltration and polarization in the epicardial adipose tissue of patients with coronary artery disease PMID: 27727089
    12. netrin-1 degradation products are capable of modulating vascular permeability in diabetic retinopathy PMID: 27400127
    13. NETRIN1 signaling pathway is identified as a candidate pathway for major depressive disorder and should be explored in further large population studies. PMID: 27422368
    14. Enhanced detection of netrin-1-expressing CD14(low) cells in patients with systemic sclerosis-related interstitial lung disease was observed, and antibody-mediated netrin-1 neutralization attenuated detection of fibrocytes in all settings. PMID: 26749424
    15. we review the Netrin-1 mediated regulation of cancer, its potential use as a biomarker, and the targeting of the Netrin-1 pathway to treat cancers PMID: 28443497
    16. Our observations revealed that SOX6 is a tumor suppressor in ovarian cancer cells, and SOX6 exerts an inhibitory effect on the proliferation, invasion, and tumor cell-induced angiogenesis of ovarian cancer cells, whereas nerin-1 plays an opposite role and its expression is inversely correlated with SOX6. PMID: 28475012
    17. NTN1 rs9788972 is identified as a risk locus for nonsyndromic orofacial clefts susceptibility in a northern Chinese population; SNPs in PAX7 were not associated with any increased risk PMID: 28489749
    18. that netrin-1 may function as a positive regulator of hypoxia-triggered malignant behavior in prostate cancer by activating the Yes-associated protein signaling PMID: 27815019
    19. Data suggest that urinary netrin-1 may be a new biomarker for determining early tubular injury in obese children. PMID: 27087488
    20. cell proliferation and survival were decreased following targeted deletion of Netrin-1. Cell invasion was dramatically diminished in Netrin-1 knockdown Glioblastoma stem cells. Moreover, Netrin-1 knockdown Glioblastoma stem cells exhibited less proangiogenic activity. PMID: 27651158
    21. Data show that netrin-1 concentrations elevated in advanced non-small cell lung cancer compared to a healthy control group, and netrin-1 concentrations decreased with chemotherapy. PMID: 27067437
    22. upregulation of netrin-1 expression in different forms of cancer, and the increased expression of netrin-1 has been linked to its functions as a survival and invasion promoting factor. PMID: 27793362
    23. High Plasma netrin-1 levels are associated with peripheral artery disease in smokers. PMID: 26944719
    24. Netrin-1 reduced the Abeta1- 40-induced Abeta1-42 increase, increased sAbetaPPalpha, and reversed the Abeta-induced sAbetaPPalpha decrease in vitro PMID: 27060954
    25. Akt-FoxO1 signaling pathway plays an essential role during liver regeneration PMID: 26573873
    26. MUC4 has a role in promoting neural invasion mediated by the axon guidance factor Netrin-1 in pancreatic ductal adenocarcinoma PMID: 26393880
    27. Netrin-1 enhanced infectivity of hepatitis C virus particles and promoted viral entry by increasing the activation and decreasing the recycling of the epidermal growth factor receptor (EGFR), a protein that is dysregulated in hepatocellular carcinoma. PMID: 27031829
    28. NTN1-DCC pathway contains targets of FDA-approved drugs and may offer promise for guiding applied clinical research on preventive and therapeutic interventions for AMD PMID: 25950802
    29. These results identify Netrin-1 as a key regulator of osteoclast differentiation that may be a new target for bone therapies. PMID: 25483983
    30. Investigation of the mechanisms that trigger local translation revealed a role for calcium-dependent retrograde netrin-1/DCC receptor signaling. PMID: 25569157
    31. Netrin-1 stimulates phosphatase 1A to dephosphorylate YAP, which leads to decreased ubiquitination and degradation, enhancing YAP accumulation and signaling PMID: 26039999
    32. results demonstrate that netrin 1 is an important regulator of blood-brain barrier maintenance that protects the central nervous system against inflammatory conditions such as multiple sclerosis. PMID: 25903786
    33. Increased maternal netrin-4 levels in IUGR neonates may reflect in utero hypoxia, while the negative correlations between fetal netrin-1 and -4 levels may exert the dynamic balance between their angio- and anti-angiogenic properties. PMID: 24716747
    34. early urinary biomarker in the diagnosis of septic acute kidney injury PMID: 25154466
    35. Studies indicate that elevating netrin-1 expression increased migration and invasion in colorectal cancer cells. PMID: 24338005
    36. These studies demonstrate that semaphorin 3A and netrin-1 can be useful early diagnostic biomarkers of AKI after liver transplantation. PMID: 25289643
    37. Netrin-1 ameliorates myocardial infarction-induced myocardial injury. PMID: 24827071
    38. results of another in vitro assay, in which endothelial cells were co-cultured with human fibroblasts, however, showed that Mel2a-netrin1 CM inhibited tube formation through blocking elongation and coalescence of human umbilical vein endothelial cells PMID: 25022751
    39. Ntn-1 induces MMP-12-dependent E-cad degradation via the distinct activation of PKCalpha and FAK/Fyn, which is necessary to govern the activation of ERK, JNK, and NF-KB in promoting motility of umbilical cord blood-derived mesenchymal stem cells. PMID: 24738865
    40. Careful phenotyping of patients combined with molecular and functional studies in zebrafish identifed netrin-1 as a potential shared genetic factor for cardiac and thyroid congenital defects. PMID: 25353184
    41. netrin-1 promoted pancreatic cancer cell proliferation by upregulation of murine double minute 2 (Mdm2). PMID: 25001177
    42. Data indicate both total and nuclear netrin-1 expression as prognostic factors in brain metastases patients in contrast to other prognostic markers in oncology such as patient age, number of brain metastases or Ki67 proliferation index. PMID: 24647424
    43. In bladder cancer cells, netrin-1 expression is increased compared to control cells. PMID: 24528886
    44. promotes angiogenesis and accelerates atherosclerosis, protects the heart against ischemia-reperfusion injury, and reduces the infarct size. PMID: 24262644
    45. Netrin-1 promotes medulloblastoma cell invasiveness and angiogenesis, and demonstrates elevated expression in tumor tissue and urine of patients with pediatric medulloblastoma. PMID: 24812271
    46. Data indicate that dysregulation of expression of netrin-1 NTN1 in smooth muscle cell (SMC) and its chemorepulsive receptor UNC5B in macrophages are involved in the development of atherosclerosis and unstable plaques. PMID: 24122613
    47. These results suggest that in hypoxic HCC cells, netrin-1 activates downstream signaling pathways to induce EMT activation with subsequent production of multiple inflammatory mediators which in turn promotes cancer invasion. PMID: 24442237
    48. The netrin-1 upregulation which appears to be p53-dependent is a survival mechanism as netrin-1 silencing by siRNA is associated with a potentiation of cancer cell death upon Doxorubicin treatment. PMID: 24293316
    49. novel model for the spatial regulation of axon branching by Netrin-1, in which localized plasma membrane expansion occurs via TRIM9-dependent regulation of SNARE-mediated vesicle fusion. PMID: 24778312
    50. Netrin-1 is highly expressed in obese but not lean adipose tissue, where it directs the retention of macrophages, promoting inflammation. NTN1 also promotes insulin resistance. PMID: 24584118

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  • 亚细胞定位：
    Secreted. Cytoplasm.
  • 组织特异性：
    Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Expressed in epididymis (at protein level).
  • 数据库链接：

    HGNC: 8029

    OMIM: 601614

    KEGG: hsa:9423

    STRING: 9606.ENSP00000173229

    UniGene: Hs.660885