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  4. Recombinant Human Nectin-1 (NECTIN1), partial

Recombinant Human Nectin-1 (NECTIN1), partial

  • 货号：
    CSB-YP621867HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP621867HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP621867HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP621867HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP621867HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CD111; CD111 antigen; CLPED1; ectodermal dysplasia 4 (Margarita Island type); ED4; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig like receptor; Herpesvirus Ig-like receptor; HIgR; HveC; MGC142031; Nectin 1; Nectin-1; OFC7; OROFACIAL CLEFT 7; OTTHUMP00000232093; OTTHUMP00000232094; OTTHUMP00000232095; Poliovirus receptor related protein 1; poliovirus receptor-like 1; Poliovirus receptor-related protein 1; PRR; PRR1; PVRL 1; PVRL1; PVRL1_HUMAN; PVRR; PVRR1; SK-12
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between NECTIN1 and NECTIN3 and between NECTIN1 and NECTIN4. Has some neurite outgrowth-promoting activity.; (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1, herpes simplex virus 2/HHV-2, and pseudorabies virus/PRV.
  • 基因功能参考文献：
    1. this is the first report showing an association between nectin1 expression and histological subtypes of gastric cancer PMID: 30221498
    2. Pseudorabies virus infects host cells via both human and swine nectin-1. PMID: 28542478
    3. In a transgenic mouse line with high expression of nectin-1Ig, significant protection from the infection with 30 and 300 MLD50 was observed . PMID: 28671524
    4. nectin-1 is expressed in human hair follicles and there is a p63-responsive element in the NECTIN1 promoter PMID: 27516130
    5. The viral entry receptor Nectin-1 is also internalized during HSV-1 infection in a Cbl-dependent mechanism, and that increases the opportunity of the virus to spread to uninfected cells. PMID: 28381567
    6. HSV gD is able to disrupt intercellular homophilic trans-interaction of nectin-1 and induce a rapid redistribution of nectin-1 from cell junctions. PMID: 27723487
    7. PVRL1 variants make a contribution to non syndromic Cleft Lip with/without Cleft palate in Turkish patients. PMID: 26953873
    8. PDZD11 forms a complex with nectin-1 and nectin-3, and its PDZ domain interacts directly with the PDZ-binding motif of nectin-1. PMID: 27044745
    9. mutations in exons 2 and 5 of PVRL1, and T334A, A391T, G1183A in the alpha-spliced transcript, and G1082T in the beta-spliced transcript do not participate in the development of non-syndromic cleft of the lip and/or palate in patients from Guangdong. PMID: 25966106
    10. data suggests that E-cadherin regulates assembly of nectin junctions through alpha-catenin-induced remodeling of the actin cytoskeleton around the cadherin clusters. PMID: 25395582
    11. The results showed that the interface between the BV gD and nectin-1 molecule is not geometrically complementary. PMID: 24902525
    12. These data provided solid structural and functional evidence that herpes simplex virus 1 and herpes simplex virus 2 gD proteins recognize nectin-1 via the same binding mode. PMID: 25231300
    13. s show that the same key residues in the BC and FG loops of nectin-4 govern binding to the measles virus attachment protein hemagglutinin (H) and cell entry, nectin-4 homodimerization, and heterodimerization with nectin-1. PMID: 25275122
    14. Host nectin-1 is required for efficient Chlamydia trachomatis serovar E development. PMID: 25414835
    15. These results suggest that HVEM might play more important roles than CD28 in ConA-mediated T cell proliferation PMID: 24163161
    16. We identified new supportive evidence that the association between PVRL1 gene and nsCL/P in Turkish patients. PMID: 24581844
    17. Glycoprotein D (gD) epitope core structure maps to the external surface of herpesvirus (HSV) gD, corresponding to the binding sites of two receptors, herpesvirus entry mediator (HVEM) and nectin-1, which mediate HSV infection. PMID: 24100313
    18. The conformation of the N-terminus of herpes simplex virus gD is induced by direct binding to HVEM and nectin-1. PMID: 24314649
    19. This study demonistated that increased expression of nectin-1 in MS lesions plays a role in the pathogenesis of MS through participation in axonal responses to injury and mediation of altered neuron-glia interactions relevant to myelination. PMID: 22460696
    20. Nectin-1 Ig3 induced phosphorylation of FGFR1c in the same manner as the whole nectin-1 ectodomain, and promoted survival of cerebellar granule neurons induced to undergo apoptosis. PMID: 22955284
    21. NSCL/P-associated mutations of PVRL1 PMID: 22455396
    22. alphaVbeta3-integrin relocalizes nectin1 to lipid rafts, independently of herpes simplex virus 1. PMID: 22171266
    23. Structure of herpes simplex virus glycoprotein D bound to the human receptor nectin-1. PMID: 21980294
    24. A nonpredicted link between SNAI1-triggered EMT and the down-regulation of Nectin-1 and StarD10 through the up-regulation of miR-661, which may contribute to the invasion of breast cancer cells and poor disease outcome. PMID: 20543867
    25. alpha- and gamma-secretase processing of nectin-1 is a Ca(2+)/calmodulin-regulated event that occurs under conditions of activity-dependent synaptic plasticity and ADAM10 and gamma-secretase are responsible for these cleavage events. PMID: 20501653
    26. There is no relationship between nonsyndromic cleft lip and/or palate and PVRL1exon3 in Han People. PMID: 19408722
    27. results suggest that PVRL1 may play a minor role in susceptibility to the occurrence of nsCL/P in some Caucasian populations, and that variation involving the beta (HIgR) isoform might have particular importance for risk of orofacial clefts PMID: 19715471
    28. evidence that nectin-1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry receptor PMID: 12072519
    29. association of HVEM and nectin-1 with lipid rafts during herpes simplex virus entry PMID: 12915568
    30. both nectin 1 and HVEM receptors play a role during HSV infection in vivo and both are highly efficient even at low levels of expression PMID: 15110526
    31. Nectin-1-Delta2 exhibited a severely reduced ability to mediate HSV entry and accumulated in the endoplasmic reticulum but retained the ability to interact with its HSV ligand, gD. PMID: 15731277
    32. trans-interacting nectin inhibits non-trans-interacting E-cadherin endocytosis through afadin, Rap1, and p120ctn and further accumulates non-trans-interacting E-cadherin to the nectin-based cell-cell adhesion sites for the formation of adherens junctions PMID: 15857834
    33. mutations at codons 185 and 323, especially the W185X mutation, do not participate in the formation of cleft lip and palate within the Taiwanese population examined PMID: 16497481
    34. Mutations within the PVRL1 gene represent risk factors for non-syndromic cleft lip with or without cleft palate. PMID: 16674562
    35. Access to nectin-1 contributes to preferential apical infection of human epithelial cells by herpes simplex virus. PMID: 17005657
    36. the role of PVRL1 in the sporadic forms of orofacial clefting in multiple populations was studied. PMID: 17089422
    37. nectin-1 may be used as a marker to predict the sensitivity of a tumor to herpes oncolytic therapy PMID: 17164781
    38. These data suggest that the determinants of gD-mediated internalization of nectin-1 may direct HSV to an endocytic pathway during entry. PMID: 18076965
    39. Novel non-synonymous PVRL1 mutation of the substitution of valine by methionine a position 395 in a conserved sequence suggests a possible etiologic role of PVRL1 in non-syndromic CL/P across different populations. PMID: 18356023
    40. Western blot analyses demonstrated that accumulation of host nectin-1 is decreased by 85 % at 48 hours post-infection (h.p.i.) in Chlamydia trachomatis serovar E-infected HeLa cells. PMID: 18451037
    41. Antibodies against nectin-1, but not HVEM, were able to block HSV-1 infection.Anti-nectin-1 antibodies and F-actin depolymerizers were also successful in blocking the cytoskeletal changes that occur upon HSV-1 entry into cells PMID: 18803666
    42. These results indicate that nectin-1 is degraded by chlamydial protease-like activity factor (CPAF) in Chlamydia trachomatis-infected genital epithelial cells, a novel strategy that Chlamydiae may use to aid their dissemination. PMID: 18983929
    43. Insertional mutations of gB reduced cell fusion activity when PILRalpha was overexpressed much more than nectin-1. PMID: 19457990
    44. Regions of nectin-1 protein important for herpesvirus entry activity and homotypic nectin-nectin interactions are overlapping but not identical. PMID: 12438620

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  • 相关疾病：
    Ectodermal dysplasia, Margarita Island type (EDMI); Non-syndromic orofacial cleft 7 (OFC7)
  • 亚细胞定位：
    [Isoform Alpha]: Cell membrane; Single-pass type I membrane protein. Cell junction, synapse, presynaptic cell membrane.; [Isoform Delta]: Cell membrane; Single-pass type I membrane protein.; [Isoform Gamma]: Secreted.
  • 蛋白家族：
    Nectin family
  • 数据库链接：

    HGNC: 9706

    OMIM: 225060

    KEGG: hsa:5818

    STRING: 9606.ENSP00000264025

    UniGene: Hs.334846