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  4. Recombinant Human Myristoylated alanine-rich C-kinase substrate (MARCKS)

Recombinant Human Myristoylated alanine-rich C-kinase substrate (MARCKS)

  • 货号：
    CSB-YP013493HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013493HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP013493HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013493HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013493HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    80 kDa protein; 80K L; 80K L protein; 80K-L protein; 80KL; 81 kDa protein; light chain; light chain; MACS; MARCKS; MARCS; MARCS_HUMAN; MGC52672; myristoylated alanine rich C kinase substrate; Myristoylated alanine rich protein kinase C substrate (MARCKS; 80K L); Myristoylated alanine rich protein kinase C substrate; Myristoylated alanine-rich C-kinase substrate; Phosphomyristin; PKCSL; PRKCSL; protein kinase C substrate 80 kDa protein light chain; Protein kinase C substrate
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-332
  • 氨基酸序列
    GAQFSKTAA KGEAAAERPG EAAVASSPSK ANGQENGHVK VNGDASPAAA ESGAKEELQA NGSAPAADKE EPAAAGSGAA SPSAAEKGEP AAAAAPEAGA SPVEKEAPAE GEAAEPGSPT AAEGEAASAA SSTSSPKAED GATPSPSNET PKKKKKRFSF KKSFKLSGFS FKKNKKEAGE GGEAEAPAAE GGKDEAAGGA AAAAAEAGAA SGEQAAAPGE EAAAGEEGAA GGDPQEAKPQ EAAVAPEKPP ASDETKAAEE PSKVEEKKAE EAGASAAACE APSAAGPGAP PEQEAAPAEE PAAAAASSAC AAPSQEAQPE CSPEAPPAEA AE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
  • 基因功能参考文献：
    1. Raman spectra show vibrational bands of Phenylalanine and Lysine residues specific for the protein effector domain, and evidence the presence of alpha helix structure in both configurations. PMID: 28866462
    2. tromal MARCKS overexpression in tumors might contribute to cancer-associated fibroblasts activation and to the poor prognosis of Epithelial ovarian cancer. PMID: 29295532
    3. Study identifies MARCKS phosphorylation at Ser46 is a hallmark of neurite degeneration, the classical hallmark of Alzheimer's disease (AD) pathology. MARCKS phosphorylation is induced by HMGB1 via TLR4. PMID: 27557632
    4. we propose a role for MARCKS in a novel mechanism of BTZ resistance via exocytosis of ubiquitinated proteins in BTZ-resistant cells leading to quenching of proteolytic stress. PMID: 27542283
    5. MARCKS overexpression might in part explain the poor prognosis of inflammatory breast cancer. PMID: 28009981
    6. s determined that myristoylated alanine-rich C-kinase substrate (MARCKS) was highly expressed in ovarian stroma, and was required for the differentiation and tumor promoting function of CAFs. PMID: 27081703
    7. Data indicate MARCKS (myristoylated alanine-rich C-kinase substrate) as a target of miR-21. PMID: 27050372
    8. data suggest a major contribution of MARCKS to kidney cancer growth and provide an alternative therapeutic strategy of improving the efficacy of multikinase inhibitors. PMID: 28166200
    9. These data suggested that miR34c3p acts as a tumor suppressor via regulation of MARCKS expression in OS progression PMID: 28075441
    10. Ca(2+)-PKC-MARCKS-PIP2-PI3K-PIP3 system functions as an activation module in vitro PMID: 27119641
    11. Findings show that calmodulin (CaM) stimulates phosphoinositide-3-kinase (PI3K) lipid kinase activity by binding MARCKS and displacing it from phosphatidylinositol 4,5-bisphosphate (PIP2) headgroups, thereby releasing free PIP2 that recruits active PI3K to the membrane and serves as the substrate for the generation of phosphatidylinositol 3,4,5-trisphosphate (PIP3). PMID: 27933776
    12. Findings suggest that MIR429 modulates mucin secretion in human colorectal cells and mouse colitis tissues by up-regulating of MARCKS expression. PMID: 26818658
    13. Knockdown of MARCKS in HepG2 cells reduced cell migration and invasion, but not cell proliferation. PMID: 26722462
    14. MARCKS upregulation increases vascular smooth muscle cell motility by activation of Rac1 and Cdc42, promoting neointima formation. PMID: 26450120
    15. A novel role for MARCKS in regulating nuclear functions such as gene expression. PMID: 26470026
    16. MARCKS knockdown arrested VSMC cell cycle by decreasing KIS expression. Decreased KIS expression resulted in nuclear trapping of p27kip1 in VSMCs. PMID: 26528715
    17. unresponsiveness of breast cancer to paclitaxel treatment is, at least in part, mediated by phospho-MARCKS PMID: 26015406
    18. MARCKS and PPP1R9A might contribute to spine loss in schizophrenia and bipolar disorder through their interactions. PMID: 25757715
    19. isotype delta-PKC is responsible for myristoylated alanine-rich C-kinase substrate (MARCKS) phosphorylation in human neutrophils following f-Met-Leu-Phe stimulation and MARCKS phosphorylation is essential for neutrophil migration and adhesion. PMID: 25515270
    20. A key role of the effector domain of MARCKS in terms of cellular response, particularly to radiation: the importance of MARCKS phosphorylation status for its subcellular localization in lung cancer. PMID: 25524703
    21. MARCKS overexpression was observed in several drug-resistant human myeloma cell lines and in drug-resistant primary multiple myeloma samples. PMID: 25179733
    22. Finding that MARCKS acts as a mediator of apoptosis in microsatellite stable colorectal cancer cells adds a novel tumor-suppressing function to the established roles of MARCKS in cell motility and proliferation. PMID: 24662837
    23. results suggest a key role for MARCKS PSD in cancer disease and provide a unique strategy for inhibiting the activity of MARCKS PSD as a treatment for lung cancer. PMID: 25318062
    24. decreased MARCKS and pMARCKS in the frontal cortex in schizophrenia was found; results suggest a mechanism other than myristoylation was responsible for decreased MARCKS expression in schizophrenia PMID: 24568864
    25. MARCKS may represent a potential biomarker for the prognosis of primary lung SCC. PMID: 24240590
    26. Phospho-MARCKS, a post-translational modification, is associated with cell motility, and has a role in the regulation of cancer cell invasiveness and metastasis. PMID: 24735036
    27. MARCKS is a negative modulator of the acrosomal exocytosis. PMID: 23704996
    28. High MARCKS expression is associated with therapeutic responsiveness in breast cancer. PMID: 23876235
    29. MARCKS plays an articulated role in the progression of colorectal cancer PMID: 23376641
    30. heat shock protein 70 (HSP70) and cysteine string protein (CSP), associate with MARCKS in the secretory mechanism in bronchial epithelial cells PMID: 23377348
    31. Cleavage ofMARCKS by Calpain may have an important role in regulation of the PKC/MARCKS pathway regulating airway mucin secretion PMID: 22710197
    32. These findings implicate that MARCKS is essential for proper cytokinesis and that MARCKS and its partner actin are key mitotic regulators during cell cycle in human hepatic stellate cells. PMID: 22555845
    33. a critical role for H(2)O(2) in angiotensin-II signaling to the endothelial cytoskeleton in a novel pathway that is critically dependent on MARCKS, Rac1, and c-Abl. PMID: 22773836
    34. Relative mRNA expression of MARCKS in white blood cells of O. viverrini-infected patients was higher than in healthy subjects; thus, MARCKS is expressed in macrophages and plays a role in inflammation-related cholangiocarcinoma induced by O. viverrini. PMID: 21763456
    35. BK promotes neurite outgrowth through transient MARCKS phosphorylation involving the PKC-dependent RhoA/ROCK pathway and PP2A in a neuroblastoma cell line. PMID: 21448919
    36. MARCKS and related chaperones bind to unconventional myosin V isoforms in airway epithelial cells PMID: 20203291
    37. reducing MRP expression promotes formation of adherens junctions in EpRas cells, allowing collective cell migration, but interferes with oncogenic beta-catenin signaling and tumorigenesis PMID: 19924305
    38. MARCKS, via its myristoylated aminoterminus, is a key regulator of neutrophil migration and adhesion. PMID: 19574534
    39. role for MARCKS as one of the key players in the migration of CCA cells and suggest that cycling between MARCKS and pMARCKS can regulate the metastasis of biliary cancer cells. PMID: 20047593
    40. Myristoylated alanine-rich C kinase substrate (MARCKS) sequesters spin-labeled phosphatidylinositol 4,5-bisphosphate in lipid bilayers. PMID: 11825894
    41. role in interaction with calmodulin PMID: 14506265
    42. MARCKS proteolysis is necessary for the fusion of myoblasts. PMID: 15239673
    43. MARCKS-mediated neurotensin release occurs via protein kinase C-delta downstream of the Rho/ROK pathwaymediated neurotensin release occurs via protein kinase C-delta downstream of the Rho/ROK pathway PMID: 15623535
    44. elevations in MARCKS expression are detrimental to specific aspects of hippocampal function PMID: 15889447
    45. These findings suggest that some PDBu-induced MARCKS phosphorylation includes the RhoA/ROCK pathway in SH-SY5Y cells. PMID: 16677610
    46. Results suggest that unphosphorylated MARCKS is involved in neurite initiation, and highlight the important role played by MARCKS in organization of the actin cytoskeleton. PMID: 16941482
    47. We suggest that the downregulation of MRP by beta3 is not required for increased cell spreading but instead that MRP downregulation is a secondary effect of increased cell spreading. PMID: 17292354
    48. PKC delta plays an important role in mucin secretion by airway epithelium via regulation of MARCKS phosphorylation. PMID: 18055557
    49. First evidence that cysteine string protein and HSP70, and their interactions with MARCKS, are involved in mucin secretion from airway epithelium. PMID: 18314541
    50. The present study indicates that MARCKS play a major key role in PDGF-BB-induced chemotaxis in activated human hepatic stellate cells. PMID: 18329017

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Membrane; Lipid-anchor.
  • 蛋白家族：
    MARCKS family
  • 数据库链接：

    HGNC: 6759

    OMIM: 177061

    KEGG: hsa:4082

    STRING: 9606.ENSP00000357624

    UniGene: Hs.519909