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  4. Recombinant Human Myoblast determination protein 1 (MYOD1)

Recombinant Human Myoblast determination protein 1 (MYOD1)

  • 货号：
    CSB-YP015358HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015358HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015358HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015358HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015358HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MYOD1
  • Uniprot No.：
  • 别名：
    bHLHc1; Class C basic helix-loop-helix protein 1; MYF 3; Myf-3; MYF3; Myoblast determination protein 1; Myod 1; MYOD; MYOD1; MYOD1_HUMAN; Myogenic differentiation 1; Myogenic factor 3; Myogenic factor MYF 3; Myogenin D1; PUM
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-320
  • 氨基酸序列
    MELLSPPLRD VDLTAPDGSL CSFATTDDFY DDPCFDSPDL RFFEDLDPRL MHVGALLKPE EHSHFPAAVH PAPGAREDEH VRAPSGHHQA GRCLLWACKA CKRKTTNADR RKAATMRERR RLSKVNEAFE TLKRCTSSNP NQRLPKVEIL RNAIRYIEGL QALLRDQDAA PPGAAAAFYA PGPLPPGRGG EHYSGDSDAS SPRSNCSDGM MDYSGPPSGA RRRNCYEGAY YNEAPSEPRP GKSAAVSSLD CLSSIVERIS TESPAAPALL LADVPSESPP RRQEAAAPSE GESSGDPTQS PDAAPQCPAG ANPNPIYQVL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
  • 基因功能参考文献：
    1. We address this paradox using basic helix-loop-helix (bHLH) transcription factors ASCL1, ASCL2, and MYOD1, crucial mediators of lineage specification..Although the ASCL factors and MYOD1 have some distinct DNA motif preference, it is not sufficient to explain the extent of the differential binding. All three factors can bind inaccessible chromatin and induce changes in chromatin accessibility and H3K27ac PMID: 29500235
    2. ACL regulates the net amount of acetyl groups available, leading to alterations in acetylation of H3(K9/14) and H3(K27) at the MYOD locus, thus increasing MYOD expression. PMID: 29241530
    3. we found that MYOD transcription factor can upregulate miR-223 expression by binding to an E-box region of the gga-miR-223 gene promoter during avian myoblast differentiation. IGF2 and ZEB1 are two target genes of miR-223 PMID: 28981085
    4. A high extent more than 25% of BRAF(V600E) alleles may be associated with disease outcome in PTC patients. PMID: 27688110
    5. we present the first report of MYOD1 (L122R) mutation in the largest cohort of 49 rhabdomyosarcomas reported so far, that are associated with a relatively aggressive clinical course PMID: 27562493
    6. Cell transdifferentiation of primary skin fibroblasts by forced expression of myogenic transcription factor MyoD was performed by quantitative analyses of gene expression and chromatin accessibility profiles. PMID: 28977539
    7. Analysis of the chromatin status of Cdkn1c promoter and KvDMR1 in unresponsive compared to responsive cell types showed that their differential responsiveness to the MyoD-dependent induction of the gene does not involve just their methylation status but, rather, the differential H3 lysine 9 dimethylation at KvDMR1. PMID: 27611768
    8. Data show that MeCP2 promotes gastric cancer (GC) cell proliferation via FOXF1-mediated Wnt5a/beta-Catenin signaling pathway, and suppresses GC cell apoptosis through MYOD1-mediated Caspase-3 signaling pathway. PMID: 28131747
    9. Our results on Pax7 and MyoD protein expression suggest that proliferation and differentiation of skeletal muscle stem cells are affected in ALS patients, and the myogenic processes cannot overcome the denervation-induced wasting. PMID: 27195289
    10. The molecular pathogenesis of radiotherapy-induced muscle fibrosis involves the TGF-beta1 pathway and its repression of MyoD expression. Our results suggest a correlation between traditional swallow therapy /neuromuscular electrical stimulation combined therapy and the restoration of TGF-beta1/MyoD homeostasis in cervical muscles. PMID: 27144672
    11. Unmethylated MYOD1 gene is associated with chemoradiation resistance in Invasive Cervical Carcinoma. PMID: 26344356
    12. We provide the first description of a human phenotype that appears to result from MYOD1 mutation. The presentation with Lethal fetal akinesia deformation sequence is consistent with a large body of data demonstrating that in the mouse, MyoD is a major controller of precursor cell commitment to the myogenic differentiation programme PMID: 26733463
    13. these results suggest that sarcoma metastasis can be partially controlled through Pax7/MyoD-dependent activation of miR-182 and provide insight into the role that myogenic transcription factors have in sarcoma progression PMID: 26234681
    14. The mechanism of bakuchiol-induced myogenesis is described. PMID: 26902638
    15. These observations demonstrated the first time that Wnt3a can directly activate MyoD expression through targeting cis-elements in the DE and the L fragment. PMID: 25651906
    16. Studies indicate that MyoD occupies multiple promoters that induce the transcription of genes vital for establishing the myogenic fate and is also implicated as a mediator of many chromatin modifying enzymes for their recruitment to myogenic enhancers. PMID: 24905980
    17. MUNC is not a classic cis-acting enhancer RNA (e-RNA) acting exclusively by stimulating the neighboring MyoD gene PMID: 25403490
    18. MyoD acts to promote SC proliferation and transition of cells into differentiation, while myogenin is known to drive terminal differentiation PMID: 25108351
    19. A recurring point mutation in MYOD1 is found in 10% of embryonal rhabdomyosarcomas with distinct clinical features and poor prognosis. PMID: 25002625
    20. Spindle cell and sclerosing rhabdomyosarcoma show recurrent MYOD1 mutations, in keeping with a single pathologic entity, regardless of age at presentation. PMID: 24824843
    21. MyoD was required for the induction of FGF21 gene transcription by mitochondrial dysfunction. PMID: 25055037
    22. analysis of a mutation in MYOD1 that may have a role in progression of embryonal rhabdomyosarcoma and may be associated with mutations altering PI3K-AKT pathway components PMID: 24793135
    23. MYOD1 homozygous mutations are frequent, recurrent and pathognomonic events in adult-type spindle cell Rhabdomyosarcoma PMID: 24272621
    24. Although expression of MyoD in a proliferating tumor is insufficient to prevent tumor progression, its expression in the cerebellum hinders medulloblastoma genesis. PMID: 24092238
    25. Direct reprogramming of fibroblasts to myocytes via bacterial injection of MyoD protein. PMID: 23438194
    26. MYOD1-transduced amnion-derived cells are capable of the dystrophin expression necessary for myogenic differentiation. PMID: 22727434
    27. CRABP2 promotes myoblast differentiation and is modulated by the transcription factors MyoD and Sp1 in C2C12 cells. PMID: 23383201
    28. SREBP-1 regulate muscle protein synthesis through the downregulation of the expression of MYOD1, MYOG and MEF2C factors. PMID: 23226416
    29. Using both primary human muscle cells and RD rhabdomyosarcoma cells, the study shows that MyoD binds in a similar genome-wide pattern in both tumor and normal cells but binds poorly at a subset of myogenic genes that fail to activate in the tumor cells. PMID: 23230269
    30. Promoter gene hypermethylation of the MYOD-1 gene increases significantly with age in normal individuals and thus may offer potential as a putative biomarker for colorectal cancer. PMID: 22591756
    31. Human squamous cell carcinomas and malignant melanomas contain significantly more Myo/Nog cells than basal cell carcinomas. PMID: 22621191
    32. results suggest that MyoD and TIP120B potentiate each other at gene expression and post-translation levels, respectively, which may promote myogenesis cooperatively PMID: 22613845
    33. the involvement of HUWE1 in the ubiquitination and proteasomal degradation of MyoD was described. PMID: 22277673
    34. BAF60c-MyoD complex directs recruitment of SWI/SNF to muscle loci in response to differentiation cues. PMID: 22068056
    35. Data demonstrate radical acceleration of iPSC creation with a fusion gene between Oct4 and the powerful transactivation domain (TAD) of MyoD. PMID: 21732495
    36. Using MYOD1, study shows that an nucleosome-depleted region at the minimal enhancer region allows reprogramming to be initiated, which occurs in response to signals such as the forced expression of Myod1 in fibroblasts. PMID: 22153073
    37. The myofibroblasts demonstrate the capacity for de-differentiation and proliferation by modulation of endogenous levels of MyoD. PMID: 21440539
    38. MyoD can play an active role in Alveolar rhabdomyosarcoma (ARMS)by augmenting Pax7-FKHR function. PMID: 21321994
    39. Increases in MYOD indicate that 1 week of conventional resistance exercise may increase myogenic activity, including satellite cell proliferation and differentiation, respectively, in younger men. PMID: 21326383
    40. Mef2d, Six4, and p38alpha MAPK function coordinately as regulators of a master regulator to mediate expression of MyoD target genes. PMID: 20716948
    41. Knockdown of MyoD and PEA3 attenuated MDR1 expression and increased the sensitivity of multidrug resistant cancer cells to cytotoxic drugs that were transported by P-gp in SGC7901/VCR cells. PMID: 20980337
    42. Transgenic Pax7 and MyoD are not essential for myogenic differentiation and participation of bone marrow-derived myogenic progenitors in muscle regeneration. PMID: 20333749
    43. calpain 3 participates in the establishment of the pool of reserve cells by decreasing the transcriptional activity of the key myogenic regulator MyoD via proteolysis independently of the ubiquitin-proteasome degradation pathway. PMID: 20139084
    44. MYOD1 hypermethylation plays an important role in colorectal cancer and may be a novel prognostic factor. PMID: 14767572
    45. MyoD modulates the rate of Id1 degradation and suggest a dynamic interplay of these factors PMID: 15163661
    46. Hypermethylation of MYOD1 is statistically significantly associated with poor disease outcome in cervical cancer. PMID: 15251938
    47. degradation is modulated by E12 and E47 PMID: 16007194
    48. This review highlights studies of molecular mechanisms by which the muscle-specific myogenic basic helix-loop-helix protein MyoD interacts with other regulatory factors to coordinate gene expression in a controlled and ordered manner. PMID: 16099183
    49. The expression of MyoD1 was more sensitive but less specific in patients with rhabdomyosarcoma. PMID: 16435141
    50. The results establish that cdk9/cyclin T2a-mediated coactivation of MyoD depends on serine 37 phosphorylation. PMID: 16841087

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    HGNC: 7611

    OMIM: 159970

    KEGG: hsa:4654

    STRING: 9606.ENSP00000250003

    UniGene: Hs.181768