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  4. Recombinant Human Myc box-dependent-interacting protein 1 (BIN1)

Recombinant Human Myc box-dependent-interacting protein 1 (BIN1)

  • 货号：
    CSB-YP002700HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002700HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002700HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002700HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002700HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    AMPH 2; AMPH2; Amphiphysin 2; Amphiphysin II; Amphiphysin like protein; amphiphysin-like; Amphiphysin-like protein; AMPHL; Bin1; BIN1_HUMAN; Box Dependant MYC Interacting Protein 1; Box-dependent myc-interacting protein 1; Bridging integrator 1; DKFZp547F068; MGC10367; MGC105358; Myc box dependent interacting protein 1; Myc box-dependent-interacting protein 1; SH3P9
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-593
  • 氨基酸序列
    AEMGSKGVT AGKIASNVQK KLTRAQEKVL QKLGKADETK DEQFEQCVQN FNKQLTEGTR LQKDLRTYLA SVKAMHEASK KLNECLQEVY EPDWPGRDEA NKIAENNDLL WMDYHQKLVD QALLTMDTYL GQFPDIKSRI AKRGRKLVDY DSARHHYESL QTAKKKDEAK IAKPVSLLEK AAPQWCQGKL QAHLVAQTNL LRNQAEEELI KAQKVFEEMN VDLQEELPSL WNSRVGFYVN TFQSIAGLEE NFHKEMSKLN QNLNDVLVGL EKQHGSNTFT VKAQPSDNAP AKGNKSPSPP DGSPAATPEI RVNHEPEPAG GATPGATLPK SPSQLRKGPP VPPPPKHTPS KEVKQEQILS LFEDTFVPEI SVTTPSQFEA PGPFSEQASL LDLDFDPLPP VTSPVKAPTP SGQSIPWDLW EPTESPAGSL PSGEPSAAEG TFAVSWPSQT AEPGPAQPAE ASEVAGGTQP AAGAQEPGET AASEAASSSL PAVVVETFPA TVNGTVEGGS GAGRLDLPPG FMFKVQAQHD YTATDTDELQ LKAGDVVLVI PFQNPEEQDE GWLMGVKESD WNQHKELEKC RGVFPENFTE RVP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Is a key player in the control of plasma membrane curvature, membrane shaping and membrane remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling. Is a negative regulator of endocytosis. Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production. In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates. May be involved in the regulation of MYC activity and the control cell proliferation. Has actin bundling activity and stabilizes actin filaments against depolymerization in vitro.
  • 基因功能参考文献：
    1. Meta-analysis validated the association of late onset Alzheimer disease with BIN1 (rs744373) variants. PMID: 29504051
    2. The findings reveal the ability of Bin1 to modify actin dynamics and provide a possible mechanistic connection between Bin1 and tau-induced pathobiological changes of the actin cytoskeleton. PMID: 28893863
    3. the association between the rs744373 polymorphism of BIN1 protein and late-onset Alzheimer's disease in East Asian, American, and European populations (Meta-Analysis) PMID: 26846281
    4. Italian family with centronuclear myopathy, carrying a novel pathogenic mutation of BIN1 gene in heterozygous state, consistent with autosomal dominant inheritance. PMID: 27854204
    5. bridging integrator 1 Gene rs7561528 polymorphism contributes to Alzheimer's disease susceptibility in East Asian and Caucasian populations PMID: 28302384
    6. Findings indicated that BIN1 restoration in NSCLC could reverse PD-L1-mediated immune escape by inactivating the c-MYC and EGFR/mitogen-activated protein kinase pathways. PMID: 28714960
    7. propose that efforts to define how genetic variants in BIN1 elevate the risk for Alzheimer's disease would behoove to consider BIN1 function in the context of its main expression in mature oligodendrocytes PMID: 27538496
    8. The results emphasize an additional level of complexity in the regulation of the interaction between BIN1 and Tau dependent on the BIN1 isoforms. PMID: 28755476
    9. Data (including data from studies using transgenic mice) suggest that the process leading to microparticle release from cardiac myocytes involves recruitment of CHMP4B protein to the forming microparticle membrane which also contains cBIN1; plasma cBIN1 is reduced in patients with heart failure as compared to control subjects. (CHMP4B = charged multivesicular body protein 4B; cBIN1 = cardiac bridging integrator 1) PMID: 28806752
    10. Low Bin1 expression is associated with esophageal squamous cell carcinoma. PMID: 28152502
    11. the depletion of BIN1 increases cellular BACE1 levels through impaired endosomal trafficking and reduces BACE1 lysosomal degradation, resulting in increased Ab production. Our findings provide a mechanistic role of BIN1 in the pathogenesis of Alzheimer disease (AD), as a novel genetic regulator of BACE1 levels and Ab production PMID: 27179792
    12. BIN1 protein expression in cerebral cortex was related to disease progression in Alzheimer's Disease patients. PMID: 27346750
    13. data show that the previously described consensus sequence PXRPXR for amphiphysin SH3 ligands is inaccurate and instead define it as an extended Class II binding motif PXXPXRpXR, where additional positive charges between the two constant arginine residues can give rise to extraordinary high SH3 binding affinity. PMID: 27268056
    14. findings support a contribution of BIN1 to individual differences in episodic memory performance among Type 2 Diabetes patients. PMID: 26947052
    15. analysis of a novel deregulated mechanism in chronic myeloid leukemia patients, indicating BIN1 and RIN1 as players in the maintenance of the abnormal RTK signaling in this hematological disease PMID: 26194865
    16. BIN1 is involved with late onset Alzheimer's disease. [review] PMID: 27773727
    17. This study supported that BIN1 contributes to the risk of Alzheimer's disease by altering neural degeneration (abnormal tau, brain atrophy and glucose metabolism) but not Abeta pathology PMID: 27003210
    18. Alterations in the BIN1 locus, previously associated with Alzheimer disease, may modify the age of onset of GBA-associated Parkinson. PMID: 26233692
    19. no association was found for either polymorphism, suggesting that these genes are not implicated in the aetiology of Alzheimer's disease in all populations. PMID: 26733302
    20. Data demonstrate that EHBP1L1 links Rab8 and the Bin1-dynamin complex, which generates membrane curvature and excises the vesicle at the endocytic recycling compartment for apical transport. PMID: 26833786
    21. In vitro studies in human Caco-2 cells showed that Bin1 antibody altered the expression of tight junction proteins and improved barrier function. PMID: 26195312
    22. This study demonistrated that BIN1 mutation releated to Centronuclear myopathy. PMID: 25957634
    23. Results show low expression of Bin1, along with high expression of IDO, are predictor for poor prognosis in esophageal squamous cell cancer and thereby could be used to establish new therapeutic strategies. PMID: 25683635
    24. The frequencies of BIN1 alleles were similar in both control and Alzheimer patients showing o no association. PMID: 26738348
    25. This study findings demonstrate that rs744373 itself or a variation in linkage disequilibrium may provide a neurogenetic mechanism for BIN.1 PMID: 25630570
    26. The study is the first to confirm the association of the variant rs7561528 adjacent to Bin1 with Sporadic Alzheimer's Disease in a Han Chinese Population. PMID: 25461955
    27. These findings suggest that an intracellular form CLU and BIN1 interaction might impact Tau function in neurons and uncover potential new mechanisms underlying the etiology of Tau pathology in in Alzheimer's disease. PMID: 25051234
    28. Reduced BIN1 expression is associated with cutaneous T-cell lymphoma. PMID: 25578476
    29. BIN1 rs744373 polymorphism is significantly associated with late onset Alzheimer disease. PMID: 25022885
    30. BIN1/M-Amphiphysin2 has a role in inducing clustering of phosphoinositides to recruit its downstream partner dynamin PMID: 25487648
    31. The release of BIN1 from hypo-poly(ADP-ribosyl)ated E2F1 is a mechanism by which serum starvation promotes E2F1-induced apoptosis. PMID: 25257171
    32. Results suggest that BIN1 is likely involved in Alzheimer's disease as a modulator of neurofibrillary tangle pathology, and that this role may extend to other human diseases that feature tau pathology PMID: 25024306
    33. These results support a role for N-WASP in amphiphysin-2-dependent nuclear positioning and triad organization and in centronuclear myopathy and myotonic dystrophy pathophysiology. PMID: 25262827
    34. specific amphiphysin 2 mutations can cause either recessive or dominant centronuclear myopathy and that both disorders involve different pathomechanisms PMID: 25260562
    35. Brain DNA methylation in BIN1 was associated with pathological Alzheimer disease. PMID: 25365775
    36. physical activity attenuated the effects of genetic risk (ie. the constellation of PICALM, BIN1, and CLU polymorphisms) on episodic memory PMID: 24660791
    37. The study compares the binding dynamics and affinities of the relevant regions for binding of c-Myc and NS5A to Bin1 SH3. The result gives further insights into the potential role of NS5A in Bin1-mediated apoptosis. PMID: 24616074
    38. These results suggest an autoinhibition model for BIN1 that involves a synergistic regulation by membrane composition and protein-protein interactions. PMID: 25350771
    39. Two mutants of BIN1 showed impaired membrane tubulation both in vivo and in vitro, and displayed characteristically different behaviors. PMID: 24755653
    40. BIN1 downregulation is linked to cancer progression and also correlates with ventricular cardiomyopathy and arrhythmia preceding heart failure. Increased BIN1 expression is linked to increased susceptibility for late-onset Alzheimer's disease. [review] PMID: 24590001
    41. Our analyses suggest that these DNA methylation changes may have a role in the onset of Alzheimer disease given that we observed them in presymptomatic subjects and that six of the validated genes connect to a known susceptibility gene network. PMID: 25129075
    42. It is the most important genetic susceptibility locus in late-onset Alzheimer's disease. PMID: 24582639
    43. BIN1 expression is increased in Alzheimer Disease brains when compared with controls. PMID: 23399914
    44. BIN1 is decreased in sporadic but not familial Alzheimer's disease or in aging PMID: 24205320
    45. REVIEW--BIN1: form, function, and Alzheimer's disease PMID: 23871436
    46. The resukts of this study highlight the possible use of plasma BIN1 as a biomarker for AD diagnosis. PMID: 23803295
    47. To our knowledge, this is the first study to show significant association between rs744373 polymorphism and AD in East Asian population. PMID: 23570733
    48. Our data demonstrate that the alteration of the muscle-specific function of BIN1 is a common pathomechanism for centronuclear myopathy, myotonic dystrophy, and IMGD. PMID: 23754947
    49. We identified rare small events overlapping CR1 and BIN1 in Alzheimer's disease and normal controls with opposite copy number variation dosage. PMID: 23202439
    50. The re-expression of BIN1 specifically compromises the proliferation of SNF5-deficient rhabdoid tumors cell lines. PMID: 22544318

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  • 相关疾病：
    Myopathy, centronuclear, 2 (CNM2)
  • 亚细胞定位：
    [Isoform BIN1]: Nucleus. Cytoplasm. Endosome. Cell membrane, sarcolemma, T-tubule.; [Isoform IIA]: Cytoplasm.
  • 组织特异性：
    Ubiquitous. Highest expression in the brain and muscle. Expressed in oligodendrocytes. Isoform IIA is expressed only in the brain, where it is detected in the gray matter, but not in the white matter. Isoform BIN1 is widely expressed with highest expressi
  • 数据库链接：

    HGNC: 1052

    OMIM: 255200

    KEGG: hsa:274

    STRING: 9606.ENSP00000316779

    UniGene: Hs.193163