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  4. Recombinant Human Mothers against decapentaplegic homolog 6 (SMAD6)

Recombinant Human Mothers against decapentaplegic homolog 6 (SMAD6)

In Stock
  • 货号：
    CSB-EP021792HU
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    hSMAD6; MAD homolog 6; MADH6; MADH7; Mothers against decapentaplegic homolog 6; Mothers against DPP homolog 6; SMAD 6; SMAD family member 6; SMAD mothers against DPP homolog 6; Smad6; SMAD6_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    60.9 kDa
  • 表达区域：
    1-496aa
  • 氨基酸序列
    MFRSKRSGLVRRLWRSRVVPDREEGGSGGGGGGDEDGSLGSRAEPAPRAREGGGCGRSEVRPVAPRRPRDAVGQRGAQGAGRRRRAGGPPRPMSEPGAGAGSSLLDVAEPGGPGWLPESDCETVTCCLFSERDAAGAPRDASDPLAGAALEPAGGGRSREARSRLLLLEQELKTVTYSLLKRLKERSLDTLLEAVESRGGVPGGCVLVPRADLRLGGQPAPPQLLLGRLFRWPDLQHAVELKPLCGCHSFAAAADGPTVCCNPYHFSRLCGPESPPPPYSRLSPRDEYKPLDLSDSTLSYTETEATNSLITAPGEFSDASMSPDATKPSHWCSVAYWEHRTRVGRLYAVYDQAVSIFYDLPQGSGFCLGQLNLEQRSESVRRTRSKIGFGILLSKEPDGVWAYNRGEHPIFVNSPTLDAPGGRALVVRKVPPGYSIKVFDFERSGLQHAPEPDAADGPYDPNSVRISFAKGWGPCYSRQFITSCPCWLEILLNNPR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transforming growth factor-beta superfamily receptors signaling occurs through the Smad family of intracellular mediators. SMAD6 is an inhibitory Smad (i-Smad) that negatively regulates signaling downstream of type I transforming growth factor-beta. Acts as a mediator of TGF-beta and BMP anti-inflammatory activities. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. Blocks the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions.
  • 基因功能参考文献：
    1. Overexpression of miR186 mimic induced HUVEC apoptosis through mitogenactivated protein kinase (MAPK) activation by targeting and inhibiting SMAD family member 6 (SMAD6). PMID: 29344637
    2. activation of AMPK upregulated Smad6 and Smurf1 and thereby enhanced their interactions, resulting in its proteosome-dependent degradation of ALK2. PMID: 28847510
    3. Results show that high Smad6 expression is correlated with increased risk of metastasis in ER negative breast cancer and that Smad6 determines BMP-regulated invasive behavior of breast cancer cells in a zebrafish xenograft model. PMID: 27113436
    4. evaluated the role of inherited genetic variation in Langerhans cell histiocytosis susceptibility and identified a novel risk variant in SMAD6 PMID: 28935696
    5. This search revealed that rare mutations that disable one copy of a gene called SMAD6 in combination with a common DNA variant near another gene called BMP2 account for about 7% of infants with midline forms of craniosynostosis. PMID: 27606499
    6. Single Nucleotide Polymorphisms in SMAD6 gene is associated with brain metastasis in non-small-cell lung cancer PMID: 23284751
    7. monoubiquitinated SMAD6 impairs the binding affinity of non-modified SMAD6 to the BMP type I receptor. Moreover, UBE2O and SMAD6 cooperated in the regulation of BMP7-induced adipogenesis PMID: 23455153
    8. Smad6 indirectly maintains stemness by preventing spontaneous erythropoiesis in hematopoietic stem cells. PMID: 22705548
    9. Loss of SMAD6 is associated with lung adenocarcinoma. PMID: 22223368
    10. Congenital cardiovascular malformation is related to genetic variation in SMAD6 PMID: 22275001
    11. Haplotype analysis further revealed that two haplotype blocks within SMAD6 were significantly associated with decreased ovarian cancer risk, as compared to the most common haplotype. PMID: 21984931
    12. results suggest that MyD88 degradation driven by the Smad6-Smurf pathway is a novel mechanism for TGF-beta1-mediated negative regulation of MyD88-dependent pro-inflammatory signalling PMID: 21897371
    13. Data show that Runx1, in conjunction with Fli1, Gata2, and Scl, directly regulates the expression of Smad6 in the aorta-gonad-mesonephros region in the developing embryo, where hematopoietic stem cells originate. PMID: 21576367
    14. Smad6 and Smad7, inhibitors of BMP signaling, were up-regulated in HFE-mutant hereditary hemochromatosis compared to controls, disruptin bone morphogenic protein signaling. PMID: 20658468
    15. Smad6 has no role in TGF-beta response and injury in podocytes. In contrast, Smad6 is upregulated in the mesangium in human glomerular diseases and may be involved in functions independent of TGF-beta/Smad signaling. PMID: 12397035
    16. Smad6 and Smad7 regulate thrombomodulin-dependent activation of protein C PMID: 12407115
    17. Smad6 repressed bone morphogenetic protein-induced Id1 transcription through recruiting transcriptional corepressor C-terminal binding protein (CtBP). PMID: 14645520
    18. tetradecanoylphorbol-13-acetate down-regulates Smad6 expression presumably via PKCmu-ERK-dependent pathway and up-regulates Smad7 expression via PKCmu-dependent mechanism(s) which need no MAPK and NF-kappaB activation PMID: 15033458
    19. The regulation in chondrocytes of Smad6 and Smad7 expression by IL-1beta suggests a potentially important role of IL-1beta signaling in chondrocytes, via indirect influencing of the bone morphogenetic protein/TGFbeta signaling cascade. PMID: 15529348
    20. Adrenomedullin can attenuate TGF-beta1-mediated renal tubulointerstitial extracellular matrix turnover via an antagonistic mechanism of inhibitory Smad 6 in TGF-beta1-elicited signaling. PMID: 15665522
    21. the level of Smad6s can alter the level of TGF-beta and the subsequent induction of PAI-1 via a FoxD1 transcription site PMID: 15716278
    22. Smad6 and Smad7 expression affects the progression of early lesions of esophageal SCC and indicates a poor prognosis. PMID: 15736400
    23. appears that the anti-glucocorticoid actions of Smad6 may contribute to the neuroprotective, anticatabolic and pro-wound healing properties of the TGFbeta family of proteins PMID: 16249187
    24. Smad6 interacts with Runx2 and mediates Smad ubiquitin regulatory factor 1-induced Runx2 degradation PMID: 16299379
    25. OAZ can alter the intensity and duration of the BMP4 stimulus through Smad6 PMID: 16373339
    26. Smad6 appears to functionally interact with Dlx3, altering the ability of Dlx3 to bind target gene promoters. PMID: 16687405
    27. Smad6 bound to Pellino-1 promoted TGF-beta-mediated anti-inflammatory effects. PMID: 16951688
    28. Stimulation of Smad 6 inhibits ERK activation and thus results in a negative feedback loop to fine-tune BMP signaling in human umbilical vein endothelial cells. PMID: 17850776
    29. A case-control study in Afro-Caribbeans found SMAD6 SNPs were not strongly associated with increased risk of developing keloids. PMID: 18445023
    30. Splice variant Smad6B, in human prostatic and rodent testicular cell lines, exhibits a truncated C-terminus lacking the entire MH-2 domain and most parts of the linker region. PMID: 19032685
    31. Knockdown of SMAD6 led to the activation of TGF-beta signaling through up-regulation of plasminogen activator inhibitor-1 and phosphorylation of SMAD2/3 in NSCLC. PMID: 19047146
    32. Smad6 gene is a candidate for the genetic determinants of bone mineral density in postmenopausal women. PMID: 19277452
    33. Study data demonstrates downregulated SMAD6 expression in psoriatic skin. PMID: 19688145

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  • 相关疾病：
    Aortic valve disease 2 (AOVD2); Craniosynostosis 7 (CRS7)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Dwarfin/SMAD family
  • 组织特异性：
    Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.
  • 数据库链接：

    HGNC: 6772

    OMIM: 602931

    KEGG: hsa:4091

    STRING: 9606.ENSP00000288840

    UniGene: Hs.153863