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  4. Recombinant Human Microtubule-associated protein RP/EB family member 2 (MAPRE2)

Recombinant Human Microtubule-associated protein RP/EB family member 2 (MAPRE2)

  • 货号：
    CSB-YP618987HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP618987HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP618987HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP618987HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP618987HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MAPRE2
  • Uniprot No.：
  • 别名：
    APC binding protein EB1; APC binding protein EB2; APC-binding protein EB2; EB1 ; EB2; End binding protein 2; End-binding protein 2; H.sapiens mRNA for novel T cell activation protein; MAPRE2; MARE2_HUMAN; Microtubule associated protein RP/EB family member 2 ; Microtubule-associated protein RP/EB family member 2; RP1 ; T cell activation protein EB1 family
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-327
  • 氨基酸序列
    MPGPTQTLSP NGENNNDIIQ DNNGTIIPFR KHTVRGERSY SWGMAVNVYS TSITQETMSR HDIIAWVNDI VSLNYTKVEQ LCSGAAYCQF MDMLFPGCIS LKKVKFQAKL EHEYIHNFKL LQASFKRMNV DKVIPVEKLV KGRFQDNLDF IQWFKKFYDA NYDGKEYDPV EARQGQDAIP PPDPGEQIFN LPKKSHHANS PTAGAAKSSP AAKPGSTPSR PSSAKRASSS GSASKSDKDL ETQVIQLNEQ VHSLKLALEG VEKERDFYFG KLREIELLCQ EHGQENDDLV QRLMDILYAS EEHEGHTEEP EAEEQAHEQQ PPQQEEY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • 基因功能参考文献：
    1. Aurora B and CDK1 temporally regulate the binding affinity of EB2 for microtubules, thereby ensuring kinetochore microtubule dynamics, proper mitotic progression and genome stability. PMID: 27030108
    2. Mutations in either TUBB or MAPRE2 cause circumferential skin creases Kunze type. PMID: 26637975
    3. EB2 is evidently important for initial microtubule reorganisation during epithelial polarisation, whereas its downregulation facilitates EB1 and ACF7 microtubule lattice association, microtubule-actin filament co-alignment and bundle formation. PMID: 23813963
    4. results suggest that EB1 and ClipCG12 act cooperatively to regulate microtubule dynamics (EB1) PMID: 22424550
    5. heterodimer formation between EB1 and EB3, but not between EB2 and the other two EBs, occurs both in vitro and in cells as revealed by live cell imaging PMID: 20008324
    6. Data suggest that a functional interaction between EB1 and p150(Glued) is required for microtubule minus end anchoring at centrosomes during the assembly and maintenance of a radial microtubule array. PMID: 12388762
    7. EB1 may modulate kinetochore microtubule polymerization and/or attachment PMID: 12475954
    8. results support the novel hypothesis that EB1 overexpression may play a role in the development of esophageal squamous cell carcinoma by affecting APC function and activating the beta-catenin/TCF pathway PMID: 16007168
    9. Impaired EB1 or APC function generates lesions invisible to the spindle checkpoint and thereby promotes low levels of chromosomal loss (CIN) expected to fuel aneuploidy and possibly tumorigenesis. PMID: 16763565
    10. These data demonstrate that the COP9 signalosome-dependent protection of EB1 is important for microtubule function. PMID: 17350042
    11. Crystal structures of the tubulin binding domains of XMAP215 (yeast Stu2p and Drosophila Msps), EB1 (yeast Bim1p and human EB1), and CLIP-170 (human), which reveal diverse tubulin binding interfaces, are reported. PMID: 17889670
    12. p150Glued may activate and thereby facilitate the recruitment of EB1 to the tips of microtubules to regulate their dynamics. PMID: 18081319
    13. EB1-tubulin interactions are mediated in part by the same tubulin acidic tails utilized by other MAPs PMID: 19778897
    14. MAPRE2 is highly expressed in pancreatic cancer cells, and seems to be involved in perineural invasion PMID: 19787265

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  • 相关疾病：
    Skin creases, congenital symmetric circumferential, 2 (CSCSC2)
  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Note=Associated with the microtubule network. Accumulates at the plus end of microtubules.
  • 蛋白家族：
    MAPRE family
  • 组织特异性：
    Expressed in different tumor cell lines. Up-regulated in activated B- and T-lymphocytes.
  • 数据库链接：

    HGNC: 6891

    OMIM: 605789

    KEGG: hsa:10982

    STRING: 9606.ENSP00000300249

    UniGene: Hs.532824