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  4. Recombinant Human Metallothionein-1A (MT1A)

Recombinant Human Metallothionein-1A (MT1A)

  • 货号：
    CSB-YP015107HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015107HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015107HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015107HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015107HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MT1A
  • Uniprot No.：
  • 别名：
    CES 1; CES1; Metallothionein 1A; Metallothionein 1S; Metallothionein 2; Metallothionein 2A; Metallothionein IA; Metallothionein II; Metallothionein-1A; Metallothionein-IA; Metallothionein2; MGC32848; MT 1A; MT 2; MT 2A; MT IA; MT II; MT-1A; MT-IA; MT1; MT1A; MT1A_HUMAN; MT1S; MT2; MT2A; MTC
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-61
  • 氨基酸序列
    MDPNCSCATG GSCTCTGSCK CKECKCTSCK KSCCSCCPMS CAKCAQGCIC KGASEKCSCC A
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
  • 基因功能参考文献：
    1. Full-length recombinant MT isoform 1a and it's isolated domain fragments were alkylated, changing protein conformation. PMID: 29518586
    2. MT1A is aberrantly silenced by DNA methylation of 5' MT1A CpG island in melanoma. PMID: 28764861
    3. Using a combination of electrospray ionization mass spectrometry (ESI-MS), circular dichroism (CD), and emission spectroscopy, this study reports that the Cu(i) to human apo-MT1A binding mechanism is highly pH-dependent. PMID: 28466911
    4. Disruption of metallothionein I and II genes in mouse produced viable mice with increased susceptibility to cadmium poisoning. PMID: 8290567
    5. Selective cysteine modification of metal-free human metallothionein 1a and its isolated domain fragments: Solution structural properties revealed via ESI-MS. PMID: 28187517
    6. blockade of metallothioneins 1 and 2 constitutes a promising approach for the treatment of conditions which result in muscle atrophy. PMID: 27956698
    7. Neonatal phthalate ester exposure induced placental MTs, FATP1 and HFABP mRNA expression PMID: 26867681
    8. The present study was undertaken to explore further the interrelationship between p53 and metallothioneins. PMID: 27049123
    9. MTF1 heads a hierarchy of zinc sensors, and through controlling the expression of a raft of metallothioneins and other key proteins involved in controlling intracellular zinc levels (e.g. ZnT1) alters zinc buffering capacity and total cellular zinc content. PMID: 26824222
    10. These results clearly suggest that MT-1 may be involved in AD pathogenesis. PMID: 26836194
    11. Zn(ii) and Cd(ii) metalation of the human MT1a takes place through two distinct pathways. PMID: 26583802
    12. We report on the competitive zinc metalation of apo-carbonic anhydrase [CA; metal-free CA (apo-CA)] in the presence of apo-metallothionein 1A domain fragments to identify domain specific determinants of zinc binding and zinc donation PMID: 26475450
    13. The Zinc and Cadmium exchange kinetics between human MT1A and carbonic anhydrase were examined using time-dependent electrospray ionization mass spectrometry. PMID: 26401817
    14. Modeling of the reactions showed that at both physiological (7.4) and acidic (5.8) pHs, zinc binding and cadmium exchanges occur essentially randomly between two MT1A fragments. PMID: 26167879
    15. Data indicate the calculated equilibrium zinc binding constants of each of the 7 zinc metallothionein 1A species ranged from a high of (log(KF)) 12.5 to a low of 11.8. PMID: 25208334
    16. Low MT1A expression is associateed with lung carcinogenesis. PMID: 23947958
    17. The metal-free, apo-alpha-MT also adopts a folded structure in the presence of the As(3+) even though there is no As(3+) bound. PMID: 24140052
    18. Increased metallothionein expression reflects steroid resistance in renal allograft recipients. PMID: 23763497
    19. Polymorphisms in the MT1A gene may influence excretion of urine uric acid and N-acetyl-beta-D-glucosamine in chronic lead-exposed workers. PMID: 23429061
    20. MT-1A is epigenetically regulated by PU.1 during monocytic differentiation. PMID: 23501100
    21. During the titration with Zn(2+), the electrospray ionization mass spectrometry data show that several metalated species coexist until the fully saturated proteins are formed. PMID: 23506369
    22. MT1A rs11076161 was associated with B-Cd concentrations and Cd-induced kidney toxicity at high exposure levels. PMID: 22995156
    23. A positive correlation between MT and Ki-67 expression was observed for all the studied cases but was even stronger in the metatypic subtype of basal cell carcinoma. PMID: 23042264
    24. Variations in the ability of LAT1/DMT1/MTF1/MT1a to process and transport Hg may not play a significant role in the etiology of autism. PMID: 21798283
    25. Possible role of MT as a marker of cell stress and homeostasis restoration in Graves'disease. PMID: 22090273
    26. The expression of metallothioneins MT1A and MT1X was significantly downregulated during differentiation of Caco-2 cells treated with high levels of zinc. PMID: 21103883
    27. MT-1A, -1F, -1G, -1X and -2A isoforms are significantly down-regulated in proliferating keloid fibroblasts. PMID: 20812968
    28. Data show that MT-I + II and megalin are significantly altered in CNS lymphoma relative to controls. PMID: 20038220
    29. Metallothionein, potential interaction partner for ECRG2, might be involved in regulation of cell proliferation and apoptosis and in various physiological processes. PMID: 12970870
    30. metallothionein measured in renal specimens from cadaver kidneys was restricted to tubular cells with no differences between controls and patients with death due to chronic diseases PMID: 15812196
    31. The overexpression of human MT1A gene dynamically affected cell viability, and the effect was influenced by zinc and cadmium ions. PMID: 16087360
    32. MT-II mRNA expression may be involved in cell proliferation in the livers of patients with chronic HCV infection. PMID: 16107899
    33. +647 MT1a genetic polymorphism may be essential for longevity in women. PMID: 16955215
    34. analysis of glial fibrillary acidic protein, metallothionein, and MHC II expression in human, rat and mouse cells PMID: 17008879
    35. The findings define a pathway for cellular metal acquisition. The results suggest a function of MT in intercellular communication. PMID: 17111383
    36. One biomarker which has recently shown to be expressed in various human tumors but still less reported in carcinoma is metallothionein. PMID: 17373731
    37. The partially metallated and metal-free metallothionein-1a species are stable intermediates and thus may have a potential role in the currently undefined function of metallothionein. PMID: 17388808
    38. The goals of this study were to define the expression of the isoforms of MT 1, 2, 3 at both mRNA and protein levels, in normal prostate, benign prostatic hyperplasia (BPH) and malignant PC-3 cells. PMID: 18208603
    39. the association of the +647 A/C MT1A polymorphism with diabetes mellitus 2 and cardiovascular complications PMID: 18249147
    40. the interleukin-6 and metallothionein 1a genes act in a concerted manner to control zinc-regulated gene expression PMID: 18316168
    41. Metal exchange in metallothioneins: a novel structurally significant Cd(5) species in the alpha domain of MT1A. PMID: 18429853
    42. This study provides the necessary data establishing unambiguously the noncooperative nature of cadmium binding to both isolated domains and the combined beta-domains (beta-rhMT) of human MT-1a. PMID: 18533113
    43. Lupus nephritis is associated with significant alterations in renal MT-I+II expression. Important prognostic information can be deduced from the renal MT-I+II expression profile in systemic lupus erythematosus patients with nephritis. PMID: 18601746
    44. MT-IA mRNA expression in HPBLs may be used as a biomarker for renal dysfunction in occupational cadmium exposure. PMID: 19359654
    45. HIF-1alpha expression qualified as an independent prognostic and characterised an aggressive cancer phenotype associated with an increased expression of MT. PMID: 19529947

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  • 蛋白家族：
    Metallothionein superfamily, Type 1 family
  • 数据库链接：

    HGNC: 7393

    OMIM: 156350

    KEGG: hsa:4489

    STRING: 9606.ENSP00000290705

    UniGene: Hs.655199