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  4. Recombinant Human Lysosomal Pro-X carboxypeptidase (PRCP)

Recombinant Human Lysosomal Pro-X carboxypeptidase (PRCP)

  • 货号：
    CSB-YP018636HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018636HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018636HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018636HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018636HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Angiotensinase C; HUMPCP ; Lysosomal carboxypeptidase C; Lysosomal Pro X carboxypeptidase; Lysosomal Pro-X carboxypeptidase; MGC2202; PCP ; PCP_HUMAN; PRCP; Proline carboxypeptidase; Prolylcarboxypeptidase (angiotensinase C); Prolylcarboxypeptidase; prolylcarboxypeptidase isoform 1 preproprotein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    46-496
  • 氨基酸序列
    KNYSV LYFQQKVDHF GFNTVKTFNQ RYLVADKYWK KNGGSILFYT GNEGDIIWFC NNTGFMWDVA EELKAMLVFA EHRYYGESLP FGDNSFKDSR HLNFLTSEQA LADFAELIKH LKRTIPGAEN QPVIAIGGSY GGMLAAWFRM KYPHMVVGAL AASAPIWQFE DLVPCGVFMK IVTTDFRKSG PHCSESIHRS WDAINRLSNT GSGLQWLTGA LHLCSPLTSQ DIQHLKDWIS ETWVNLAMVD YPYASNFLQP LPAWPIKVVC QYLKNPNVSD SLLLQNIFQA LNVYYNYSGQ VKCLNISETA TSSLGTLGWS YQACTEVVMP FCTNGVDDMF EPHSWNLKEL SDDCFQQWGV RPRPSWITTM YGGKNISSHT NIVFSNGELD PWSGGGVTKD ITDTLVAVTI SEGAHHLDLR TKNALDPMSV LLARSLEVRH MKNWIRDFYD SAGKQH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
  • 基因功能参考文献：
    1. For the first time, PRCP is identified as an apelin-cleaving enzyme. PMID: 27449720
    2. The decrease in PRCP levels in the first 24 h after stroke onset is associated with stroke severity and an unfavourable short-term stroke outcome PMID: 25370794
    3. PRCP1 interacts with plasma kallikrein (PK) at multiple sites for PK activation. PMID: 25324000
    4. The present results indicated PRCP rs7104980 can be considered as a marker for EH and Hap3 GAGCACTAACA (PRCP) and Hap16 TTTA (CMA1) might be associated with essential hypertension in Chinese Han population. PMID: 22679278
    5. PRCP regulates cell growth, angiogenesis, and the response to vascular injury PMID: 23744584
    6. analysis of non-benzimidazole and brain-penetrant prolylcarboxypeptidase inhibitors PMID: 22079761
    7. PRCP as a resistance factor for 4OHTAM resistance in estrogen receptor-positive breast cancer cells. PMID: 21087932
    8. A single peptide, with the sequence YPRPIHPA, as a novel substrate for PRCP in human cerebrospinal fluid. PMID: 20517885
    9. Purified PrCP yielded crystals belonging to space group R32, with unit-cell parameters a = b = 181.14, c = 240.13 A, that diffracted to better than 2.8 A resolution. PMID: 20516604
    10. A structure-based alignment with the previously undescribed structure of DPP7 illuminates the mechanism of orthogonal substrate specificity of PRCP and DPP7. PMID: 20540760
    11. Data suggest that the E112D polymorphism in the PRCP gene may be a useful genetic marker to predict the antihypertensive effect of short-term benazepril treatment in hypertensive patients. PMID: 20079160
    12. PRCP appears to be a HUVEC-associated prekallikrein activator. PMID: 11830581
    13. Identification of prolylcarboxypeptidase as the cell matrix-associated prekallikrein activator. PMID: 12123826
    14. recombinant protein, identical to enzyme from human umbilical vein endothelial cells, is a prekallikrein activator. PMID: 14996700
    15. Prolylcarboxypepdiase E112D (rs2298668)D allele alone and jointly with chronic hypertension were associated with a significantly increased risk of preeclampsia PMID: 16681991
    16. role in regulation cardiovascular tone; proinflammatory agent PMID: 18396440
    17. These investigations showed that the C-terminal region of the rPRCP(40) contributes to PRCP's catalytic function, and provided additional experimental evidence for this suggestion. PMID: 18656443

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  • 亚细胞定位：
    Lysosome.
  • 蛋白家族：
    Peptidase S28 family
  • 组织特异性：
    Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney.
  • 数据库链接：

    HGNC: 9344

    OMIM: 176785

    KEGG: hsa:5547

    STRING: 9606.ENSP00000377055

    UniGene: Hs.523936