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  4. Recombinant Human Lysine-specific demethylase NO66 (NO66)

Recombinant Human Lysine-specific demethylase NO66 (NO66)

  • 货号：
    CSB-YP887992HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP887992HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP887992HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP887992HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP887992HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RIOX1
  • Uniprot No.：
  • 别名：
    60S ribosomal protein L8 histidine hydroxylase; C14orf169; Chromosome 14 open reading frame 169 ; Histone lysine demethylase NO66; hsNO66; Hypothetical protein FLJ21802; Lysine-specific demethylase NO66; MAPJD; MYC associated protein with JmjC domain; NO66; NO66_HUMAN; Nucleolar protein 66; Ribosomal oxygenase NO66; ROX; Up regulated in lung cancer 2; URLC2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-641
  • 氨基酸序列
    MDGLQASAGP LRRGRPKRRR KPQPHSGSVL ALPLRSRKIR KQLRSVVSRM AALRTQTLPS ENSEESRVES TADDLGDALP GGAAVAAVPD AARREPYGHL GPAELLEASP AARSLQTPSA RLVPASAPPA RLVEVPAAPV RVVETSALLC TAQHLAAVQS SGAPATASGP QVDNTGGEPA WDSPLRRVLA ELNRIPSSRR RAARLFEWLI APMPPDHFYR RLWEREAVLV RRQDHTYYQG LFSTADLDSM LRNEEVQFGQ HLDAARYING RRETLNPPGR ALPAAAWSLY QAGCSLRLLC PQAFSTTVWQ FLAVLQEQFG SMAGSNVYLT PPNSQGFAPH YDDIEAFVLQ LEGRKLWRVY RPRVPTEELA LTSSPNFSQD DLGEPVLQTV LEPGDLLYFP RGFIHQAECQ DGVHSLHLTL STYQRNTWGD FLEAILPLAV QAAMEENVEF RRGLPRDFMD YMGAQHSDSK DPRRTAFMEK VRVLVARLGH FAPVDAVADQ RAKDFIHDSL PPVLTDRERA LSVYGLPIRW EAGEPVNVGA QLTTETEVHM LQDGIARLVG EGGHLFLYYT VENSRVYHLE EPKCLEIYPQ QADAMELLLG SYPEFVRVGD LPCDSVEDQL SLATTLYDKG LLLTKMPLAL N
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation. May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.
  • 基因功能参考文献：
    1. our findings clearly indicated that epigenetic regulation by NO66 plays an important role in cancer metastatic processes in CRC. PMID: 27473587
    2. confirmed the hydroxylase activity of NO66 and showed that oligomerization is required for NO66 to efficiently catalyze the hydroxylation of Rpl8 PMID: 26327385
    3. hinge domain-dependent oligomerization of NO66 is essential for inhibition of Osx-dependent gene activation. PMID: 23620590
    4. NO66 crystallized in space group P3(1) or P3(2), with unit-cell parameters a = 89.35, b = 89.35, c = 304.86 A, alpha = beta = 90, gamma = 120 degrees , and the crystal is likely to contain four molecules in the asymmetric unit PMID: 22750859
    5. interactions between NO66 and Osx regulate Osx-target genes in osteoblasts by modulating histone methylation states PMID: 19927124
    6. NO66 has functions in ribosome biogenesis and in the replication or remodeling of certain heterochromatic regions PMID: 14742713

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  • 亚细胞定位：
    Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Granular part of nucleoli. Nucleoplasm, nucleoplasmic foci, some of them associated with nucleoli.
  • 蛋白家族：
    ROX family, NO66 subfamily
  • 组织特异性：
    Widely expressed. Overexpressed in lung carcinomas.
  • 数据库链接：

    HGNC: 20968

    OMIM: 611919

    KEGG: hsa:79697

    UniGene: Hs.509916