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  4. Recombinant Human Lupus La protein (SSB)

Recombinant Human Lupus La protein (SSB)

  • 中文名称：
    人SSB重组蛋白
  • 货号：
    CSB-YP022705HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SSB重组蛋白
  • 货号：
    CSB-EP022705HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SSB重组蛋白
  • 货号：
    CSB-MP022705HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SSB
  • Uniprot No.：
  • 别名：
    Autoantigen La; La ; La autoantigen; La autoantigen homolog; La protein; La ribonucleoprotein; La ribonucleoprotein domain family member 3; LA_HUMAN; LARP3; Lupus La antigen; Lupus La protein; Lupus La protein homolog ; MGC118101; MGC93380; mRNA for autoantigen; OTTMUSP00000014043; RP23 273G23.1; Sjoegren syndrome type B antigen; Sjogren syndrome antigen B (autoantigen La); Sjogren syndrome antigen B; SS B; SS-B; SS-B/La protein ; SSB
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-408
  • 氨基酸序列
    MAENGDNEKM AALEAKICHQ IEYYFGDFNL PRDKFLKEQI KLDEGWVPLE IMIKFNRLNR LTTDFNVIVE ALSKSKAELM EISEDKTKIR RSPSKPLPEV TDEYKNDVKN RSVYIKGFPT DATLDDIKEW LEDKGQVLNI QMRRTLHKAF KGSIFVVFDS IESAKKFVET PGQKYKETDL LILFKDDYFA KKNEERKQNK VEAKLRAKQE QEAKQKLEED AEMKSLEEKI GCLLKFSGDL DDQTCREDLH ILFSNHGEIK WIDFVRGAKE GIILFKEKAK EALGKAKDAN NGNLQLRNKE VTWEVLEGEV EKEALKKIIE DQQESLNKWK SKGRRFKGKG KGNKAAQPGS GKGKVQFQGK KTKFASDDEH DEHDENGATG PVKRAREETD KEEPASKQQK TENGAGDQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation. In case of Coxsackievirus B3 infection, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation.
  • 基因功能参考文献：
    1. A cytoplasmic isoform of La protein as well as LARPs 6, 4, and 1 function in mRNA metabolism and translation in distinct but similar ways, sometimes with the poly(A)-binding protein, and in some cases by direct binding to poly(A)-RNA. PMID: 28782243
    2. our data support a novel model, whereby cancer-associated La protein contributes to cisplatin resistance by stimulating the translation of anti-apoptotic factor Bcl2 in HNSCC cells. PMID: 27105491
    3. La protein is an important microprocessor component regulating miRNA processing efficiency by association with DGCR8 to regulate formation of the DGCR8-Drosha complex for miRNA processing. PMID: 29087193
    4. La functions as gatekeeper ensuring correct tRNA maturation and protecting the miRNA pathway from potentially functional tRNA fragments. PMID: 27345152
    5. Findings identified two novel SUMO acceptors sites on La and suggest that sumoylation of La alters its RNA-binding activity, which could influence a broad range of RNA processing steps where La's function has been implicated. PMID: 27224031
    6. Binding of double-stranded RNA involves less penetration into the RNA recognition motif (RRM2) binding pocket and more engagement with the unstructured C-terminus of the La C-terminal domain (CTD). PMID: 27959512
    7. At the 3' UTR, HUR facilitates circularization of the viral genome through interaction with another host factor, La, which is critical for replication. PMID: 26339049
    8. expression of nuclear hLa Ag induces thymic clonal deletion and tTreg selection, and lymphocytic infiltration of the lung is a consequence of La-specific CD4(+) T cell autoimmunity PMID: 25582858
    9. s provide a model whereby a novel interplay between RNA-binding, RNA chaperoning and AKT phosphorylation of La protein regulates CCND1 IRES-mediated translation. PMID: 25520193
    10. Primary Sjogren's patients with WSP form a benign subgroup, with a lower prevalence of anti-SSB and extraglandular manifestations. PMID: 24529195
    11. A beta turn in the human La protein is responsible for the interaction with the hepatitis C virus RNA. PMID: 24478427
    12. Total IgG levels correlate positively with autoantibodies La, Ro60 and Ro52 but not with RING or B-box domain antibodies of Ro52. PMID: 23554036
    13. The competition between the host factor (La) and the viral protein (NS3) for binding to HCV IRES might regulate the molecular switch from translation to replication of HCV. PMID: 22355520
    14. Our study demonstrates that La plays a critical role in regulating HCV replication by interacting with the GCAC motif. PMID: 23552417
    15. Sjogren syndrome antigen B (SSB)/La promotes global microRNA expression by binding microRNA precursors through stem-loop recognition PMID: 23129761
    16. Iron promotes the translation initiation of hepatitis C virus by stimulating the expression of eIF3A and La proteins. PMID: 22634302
    17. oxidants cause nuclear export of La/SSB protein and subsequent association of La/SSB with Nrf2 untranslated regions and ribosomes PMID: 22207702
    18. data indicate that the JAK2(V617F) mutation affects p53 response to DNA damage through the upregulation of La antigen and accumulation of MDM2 PMID: 21785463
    19. the structural elements required for La-mediated RNA chaperone activity PMID: 22203678
    20. La binds to domain IV of the hepatitis C virus RNA using an RNA recognition that is quite distinct from its mode of binding to RNAs with a 3' UUU(OH) trailer. PMID: 22009680
    21. function of the RNA-binding protein La in promoting tumor progression of head and neck squamous cell carcinoma PMID: 22016766
    22. Autoantigen La promotes efficient RNAi, antiviral response, and transposon silencing by facilitating multiple-turnover RNA-induced silencing complex catalysis. PMID: 22055194
    23. Overexpression of mouse or human La enhanced M-MuLV particle release in the absence of glycosylated gag. PMID: 21343359
    24. La protein binds to and mediates interaction of the 5' and 3' noncoding ends of the Japanese encephalitis virus genome. PMID: 21294637
    25. show elevated La protein expression in cervical cancer tissue and its correlation with aberrant CCND1 protein levels in cervical tumor tissue lysates PMID: 20856207
    26. There is a correlation between La protein and HBV mRNA and the expression of HBV protein. PMID: 17064465
    27. study found expression of La protein is induced by HCV infection, and this induced La protein-activated telomerase activity in a hepatoma cell line; HCV infection might be strongly related to telomerase activity in the liver through La protein induction PMID: 20497049
    28. nuclear trafficking by La affects the normal order of pre-tRNA processing PMID: 12049746
    29. molecular characterization of the human La protein.hepatitis B virus RNA.B interaction in vitro PMID: 12121976
    30. La autoantigen is required for the internal ribosome entry site-mediated translation of Coxsackievirus B3 RNA PMID: 12384597
    31. binds differentially to multiple sites within the 5' untranslated region of Coxsackievirus B3 RNA PMID: 12457960
    32. in addition to full-length La protein, both N- and C-terminal halves were able to interact with hepatitis C virus internal ribosome entry site in vivo. PMID: 12540850
    33. Dengue 4 virus minus strand 3'UTR RNA binds with La protein in human monocytes PMID: 12584332
    34. Results suggest that La ribonucleoproteins (RNP) exist in distinct states that differ in subcellular localization, serine 366 phosphorylation, and associated RNAs. PMID: 14636586
    35. La motif adopts an alpha/beta fold that comprises a winged-helix motif elaborated by the insertion of three helices PMID: 15004549
    36. La protein binds to the 5' end and with the 3' UTR of Dengue virus RNA; interacts with the dengue virus nonstructural proteins NS5 and NS3 PMID: 15084396
    37. NMR assignment and secondary structure of the La motif PMID: 15213463
    38. Composition of La/hepatitis B virus ribonucleoprotein particles as well as interacting cellular factors, are critical determinants in the regulation of the stability of the HBV RNA. PMID: 15302879
    39. RRM1 and La motifs bind pre-tRNA PMID: 15371415
    40. CK2 is responsible for La S(366) phosphorylation in vivo PMID: 15485924
    41. Nonphosphorylated SSB interacts with nucleolin at nucleolar sites involved in rRNA biogenesis. PMID: 15572691
    42. This protein is s host factors responsible for the regulation of the hepatitis C virus internal ribosome entry site. PMID: 15685555
    43. essential for efficient hepatitis C virus replication PMID: 15823607
    44. In vitro RNA chaperone activity pf SSB was studied. PMID: 15928345
    45. nuclear retention of peptidylglycine alpha-amidating monooxygenase (PAM) mRNA is lost upon expressing the La proteins that lack a conserved nuclear retention element, suggesting a direct association between PAM mRNA and La protein in vivo PMID: 16107699
    46. Anti-La antibodies bound to immunodominant epitopes of La within the NH(2)-terminus and the RNA recognition motif (RRM) region of apoptotic human cells PMID: 16320341
    47. Here, we show by chromatin immunoprecipitation (ChIP) that La is associated with pol III-transcribed genes in vivo PMID: 16344466
    48. Results report the crystal structure of the N-terminal domain of human La, consisting of La and RRM1 motifs, bound to RNA. PMID: 16387655
    49. Changes in salivary production rate are associated with aging and SSB antibodies. PMID: 16467037
    50. La protein undergoes nucleocytoplasmic shuttling whereas EBER1 and EBER2 noncoding RNA's are confined to the nucleus. PMID: 16682524

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    HGNC: 11316

    OMIM: 109090

    KEGG: hsa:6741

    STRING: 9606.ENSP00000260956

    UniGene: Hs.632535