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  4. Recombinant Human Kunitz-type protease inhibitor 1 (SPINT1)

Recombinant Human Kunitz-type protease inhibitor 1 (SPINT1)

  • 货号：
    CSB-YP022584HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP022584HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022584HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022584HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022584HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SPINT1
  • Uniprot No.：
  • 别名：
    HAI 1; HAI; HAI-1; HAI1; Hepatocyte growth factor activator inhibitor type 1; Kunitz type protease inhibitor 1; Kunitz-type protease inhibitor 1; MANSC2; Serine peptidase inhibitor Kunitz type 1; SPINT1; SPIT1_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    36-529
  • 氨基酸序列
    GPPPA PPGLPAGADC LNSFTAGVPG FVLDTNASVS NGATFLESPT VRRGWDCVRA CCTTQNCNLA LVELQPDRGE DAIAACFLIN CLYEQNFVCK FAPREGFINY LTREVYRSYR QLRTQGFGGS GIPKAWAGID LKVQPQEPLV LKDVENTDWR LLRGDTDVRV ERKDPNQVEL WGLKEGTYLF QLTVTSSDHP EDTANVTVTV LSTKQTEDYC LASNKVGRCR GSFPRWYYDP TEQICKSFVY GGCLGNKNNY LREEECILAC RGVQGGPLRG SSGAQATFPQ GPSMERRHPV CSGTCQPTQF RCSNGCCIDS FLECDDTPNC PDASDEAACE KYTSGFDELQ RIHFPSDKGH CVDLPDTGLC KESIPRWYYN PFSEHCARFT YGGCYGNKNN FEEEQQCLES CRGISKKDVF GLRREIPIPS TGSVEMAVAV FLVICIVVVV AILGYCFFKN QRKDFHGHHH HPPPTPASST VSTTEDTEHL VYNHTTRPL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14).
  • 基因功能参考文献：
    1. The SPINT1 expression was associated with extrathyroidal invasion, lymphovascular invasion, lymph node metastasis, advanced TNM stage, and a higher risk of recurrence in differentiated thyroid cancer. PMID: 29532159
    2. HAI-1 is upregulated in pancreatic intraepithelial neoplasia and broadly expressed in pancreatic ductal adenocarcinoma (PDAC) cells. However, PDAC cases having areas of reduced HAI-1 immunoreactivity may show shorter disease-free survival. PMID: 29202869
    3. In this work, KLK14 binding to either hepatocyte growth factor activator inhibitor type-1 (HAI-1) or type-2 (HAI-2) was essayed using homology modeling, molecular dynamic simulations and free-energy calculations through MM/PBSA and MM/GBSA. KLK14 was successfully modeled. PMID: 28817220
    4. the cell surface expression of HAI-1 in all viable epidermal layers renders it an effective regulator for matriptase and prostasin PMID: 29438412
    5. Data suggest that HAI1, a protease on the surface of colon carcinoma cells, is an MMP7 substrate; proteolysis by MMP7 releases extracellular region as soluble HAI1 (sHAI1); sHAI1 induces cancer cell aggregation; cholesterol sulfate is required for MMP-7--catalyzed generation of sHAI1. (HAI1 = hepatocyte growth factor activator inhibitor type 1; MMP7 = matrix metalloproteinase-7) PMID: 29046355
    6. Concomitant presence of TMPRSS13 with HAI-1 mediates phosphorylation of residues in the intracellular domain of the protease, and it coincides with efficient transport of the protease to the cell surface and its subsequent shedding. PMID: 28710277
    7. our data illustrated a novel pathway comprising miR-221and miR-222 and hepatocyte growth factor activator inhibitor type 1 in gastric cancer, which is a potential target for future clinical use PMID: 28618968
    8. DNA methylation levels in the COASY and SPINT1 promoter regions were considered to potentially be a convenient and useful biomarker for diagnosis of Alzheimer's Disease and Amnestic Mild Cognitive Impairment. PMID: 27992572
    9. The structural insights gained here improve our understanding of the regulation of HAI-1 inhibitory activities and point to new approaches for better controlling these activities. PMID: 28348076
    10. The present study demonstrated that ovarian cancer cell metastasis and invasion were more dependent on upregulation of matriptase levels than downregulation of HAI1. Matriptase may be a potential adjuvant therapeutic target for inhibiting ovarian cancer invasion and metastasis. PMID: 27356668
    11. provide new insight into the interplay between tertiary structure and the inhibitory activity of a multidomain protease inhibitor PMID: 27226587
    12. The findings suggest that the oncogenic activity of hepsin arises not only from elevated expression level but also from depletion of HAI-1, events which together trigger gain-of-function activity impacting HGF/MET signalling and epithelial cohesion PMID: 26165838
    13. it was found that the recombinant fusion protein uPA17-34-KPI (kunitz-type protease inhibitor ) inhibited the invasion and metastasis of ovarian tumors PMID: 26166362
    14. HAI-1 may have a critical role in maintaining normal keratinocyte morphology through regulation of PAR-2-dependent p38 mitogen-activated protein kinase signaling. PMID: 25842366
    15. the anti-migratory effects seen via gamma-catenin were driven by the expression of hepatocyte growth factor activator inhibitor Type I (HAI-1 or SPINT-1), an upstream inhibitor of the c-MET signaling pathway. PMID: 25925948
    16. Data suggest HAI1 exhibits structural features consistent with classification in MANEC subclass of PAN/apple domain family with unifying features such as two additional disulfide bonds, two extended loop regions, and additional alpha-helical elements. PMID: 25510835
    17. Matriptase activity was suppressed by the expression of HAI-1. PMID: 24147538
    18. The results of the present study identified HAI-1 as a favorable prognostic marker for prostate cancer and may indicate that HAI-1 could be a therapeutic target for the treatment of this malignancy. PMID: 24659667
    19. HAI-1 expression correlates with the differentiation status of colorectal epithelia and could serve as a differentiation marker. PMID: 23979832
    20. HAI-1 and HAI-2 influence proliferation and cell fate of NPCs and their expression levels are linked to BMP sign PMID: 23409135
    21. Crystal structures of matriptase in complex with its inhibitor hepatocyte growth factor activator inhibitor-1 PMID: 23443661
    22. It is suggested that HAI-1 may play an important role in the pathogenesis of castration-resistant prostate cancer PMID: 23393351
    23. These results suggest that HAI-1 functions as a physiological regulator of HAT by inhibiting its protease activity and proteolytic activation in airway epithelium.(HAI-1) PMID: 22023801
    24. we showed for the first time that MT1-MMP activates matriptase through the cleavage of HAI-1 PMID: 22118498
    25. These data suggest that matriptase activity can be rapidly inhibited by HAI-1 and other HAI-1-like protease inhibitors and "locked" in an inactive autoactivation intermediate, all of which places matriptase under very tight control. PMID: 22031598
    26. HAI-1 and -2 are overexpressed in the liver in cholangiopathies with ductular reactions and are possibly involved in liver fibrosis and hepatic differentiation. PMID: 21898507
    27. The data suggested that normal epithelial cells use a dual mechanism involving hepatocyte growth factor activator inhibitor-1 and antithrombin to control matriptase and that the antithrombin-based mechanism is lost in the majority of carcinomas. PMID: 21795523
    28. Low HAI-1 is associated with endometrial cancer. PMID: 20715109
    29. High-level expression of HAI-1 is retained in the epidermal granular layer. This pattern of expression is retained in most skin disorders. PMID: 21123732
    30. Expression of HAI-1 in hepatocellular carcinoma is associated with poor prognosis. HAI-1 may be important in neoplasm progression and a new prognostic factor for hepatocellular carcinoma. PMID: 20213201
    31. HAI-1 regulates pulmonary metastasis of the human pancreatic carcinoma cell line SUIT-2 PMID: 21166957
    32. results suggest that TMPRSS13 functions as an HGF-converting protease, the activity of which may be regulated by HAI-1 PMID: 20977675
    33. the matriptase-prostasin proteolytic cascade is tightly regulated by two mechanisms: 1) prostasin activation temporally coupled to matriptase autoactivation and 2) HAI-1 rapidly inhibiting not only active matriptase but also active prostasin PMID: 20696767
    34. Data suggest that over-expression of HAI-1 in SW620 cells has weak effect on cell growth in vitro, but can significantly inhibit cell migration/motility. PMID: 16044910
    35. hepatocyte growth factor activator inhibitor-1B (new isoform) has roles in various physiological and pathological processes PMID: 12815039
    36. 1 serine protease inhibitor genotype is a significant risk factor for chronic obstructive pulmonary disease. PMID: 15994391
    37. HAI-1B is a potential physiological regulator of prostasin function PMID: 16103126
    38. The expressions of SNC19/matriptase and its inhibitor HAI-1 are decreased in gastrointestinal cancer tissues PMID: 16273651
    39. results show that dysregulation of the matriptase/HAI-1 mRNA ratio occurs early during colorectal cancer carcinogenesis PMID: 16820046
    40. Addition of HAI1 blocked the proteolytic activation of STP14 at the cell surface of peritoneal macrophages. PMID: 17389401
    41. Prostate cancer cells, after loss of HAI-1, had a significantly increased in vitro invasiveness together with an increase in cellular motility. PMID: 17786295
    42. analysis of the regulation of matriptase and HAI-1 with an emphasis on the molecular mechanisms governing its zymogen activation, inhibition by HAI-1, and ectodomain shedding [review] PMID: 17981575
    43. Unlike HAI-1 and matriptase, HAI-2 expression is detected in non-epithelial cells of brain and lymph nodes, suggesting that HAI-2 may also be involved in inhibition of serine proteases other than matriptase PMID: 18713750
    44. tissue microenvironment regulates the cell surface expression of HAI-1, and thereby may regulate proteolysis and processing of bioactive molecules on the cellular surface PMID: 18769935
    45. HAI-1 expression levels were significantly higher in all proliferative prostate diseases and seems to be a marker of prostate epithelial cell proliferation PMID: 18813126
    46. HAI-1 and HAI-2 may possibly be therapeutic target for treatment approaches in ovarian cancer. PMID: 19148468
    47. Expression of hepatocyte growth factor activator inhibitor type 1 in bronchial epithelial cells are altered by tissue injury to bronchi. PMID: 19222607
    48. interactions between HAI-1/SPINT1 and membrane-bound serine proteinases contribute to transcriptional and functional changes involved in EMT in certain carcinoma cells. PMID: 19223533
    49. Data suggest that the cellular location of matriptase activation and inhibition, and the secretory route for matriptase-HAI-1 complex may vary along with the functional divergence of different epithelial cells. PMID: 19535514
    50. The disease-free and overall survival rates of patients exhibiting high HAI-1 expression were significantly higher than those of patients exhibiting low HAI-1 expression in cervix cancer. PMID: 19578736

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  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    HGNC: 11246

    OMIM: 605123

    KEGG: hsa:6692

    STRING: 9606.ENSP00000342098

    UniGene: Hs.233950