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  4. Recombinant Human Kinetochore protein Nuf2 (NUF2)

Recombinant Human Kinetochore protein Nuf2 (NUF2)

  • 货号：
    CSB-YP871623HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP871623HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP871623HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP871623HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP871623HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NUF2
  • Uniprot No.：
  • 别名：
    Cancer/testis antigen 106; CDCA 1; CDCA1; Cell division cycle associated 1; Cell division cycle associated protein 1; Cell division cycle-associated protein 1; CT106; hNuf 2; hNuf2; hNuf2R; hsNuf2; Kinetochore protein Nuf2; Nuf 2; NUF2; NUF2 NDC80 kinetochore complex component ; NUF2 NDC80 kinetochore complex component homolog ; NUF2, S. cerevisiae, homolog of; NUF2_HUMAN; NUF2R
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-464
  • 氨基酸序列
    METLSFPRYN VAEIVIHIRN KILTGADGKN LTKNDLYPNP KPEVLHMIYM RALQIVYGIR LEHFYMMPVN SEVMYPHLME GFLPFSNLVT HLDSFLPICR VNDFETADIL CPKAKRTSRF LSGIINFIHF REACRETYME FLWQYKSSAD KMQQLNAAHQ EALMKLERLD SVPVEEQEEF KQLSDGIQEL QQSLNQDFHQ KTIVLQEGNS QKKSNISEKT KRLNELKLSV VSLKEIQESL KTKIVDSPEK LKNYKEKMKD TVQKLKNARQ EVVEKYEIYG DSVDCLPSCQ LEVQLYQKKI QDLSDNREKL ASILKESLNL EDQIESDESE LKKLKTEENS FKRLMIVKKE KLATAQFKIN KKHEDVKQYK RTVIEDCNKV QEKRGAVYER VTTINQEIQK IKLGIQQLKD AAEREKLKSQ EIFLNLKTAL EKYHDGIEKA AEDSYAKIDE KTAELKRKMF KMST
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.
  • 基因功能参考文献：
    1. CDCA1 could be a prognostic marker in malignant melanoma patients. PMID: 27237743
    2. Results provide evidence that CDCA1 plays a significant role in oral cavity carcinoma (OCC) tumorigenesis. Importantly, CDCA1 is associated with growth and survival of OCC cells where its elevated expression issignificantly associated with poor prognosis . PMID: 27499128
    3. NUF2 modulates cell proliferation via cell cycle control in osteosarcoma Saos-2 cells. PMID: 27049259
    4. found LncRNA AF339813 was positively regulated by NUF2 PMID: 26045769
    5. NUF2 silencing by RANi (RNA interference) reduced the proliferation and colony formation ability of pancreatic cancer cells in vitro. PMID: 25370920
    6. NUF2 plays a critical role in the regulation of HCC cell proliferation and apoptosis. PMID: 25374179
    7. NUF2 was overexpressed in glioma tissues and differentially expressed in a series of glioma cell lines. Depletion of NUF2 by short-hairpin RNA inhibited cell growth in vitro and in vivo. PMID: 25481014
    8. hnRNP K binds to the promoter region of NUF2 and activates its transcription. PMID: 25701787
    9. These results suggest that CDCA1 could be a prognostic biomarker and a potential therapeutic target for colorectal cancers . PMID: 24247253
    10. these findings demonstrated that three buried glutamic acid-lysine pairs, in concert with hydrophobic interactions of core residues, provide the major specificity and stability requirements for Hec1-Nuf2 dimerization PMID: 24129578
    11. NDC80, NUF2 and PTN were significantly aberrantly overexpressed in serous adenocarcinomas. PMID: 23056589
    12. These data suggest that the CH and tail domains of Hec1 generate essential contacts between kinetochores and microtubules in cells, whereas the Nuf2 CH domain does not. PMID: 21270439
    13. cell growths of colorectal and gastric cancers after the siRNA-mediated knockdown of either CDCA1 or KNTC2 were significantly suppressed. PMID: 19878654
    14. Data highlight a specific role for hNuf2 in kinetochore-microtubule attachment and suggest that hNuf2 is part of a molecular linker between the kinetochore attachment site and tubulin subunits within the lattice of attached plus ends. PMID: 12438418
    15. NUF2 is a core component of the kinetochore outer plate essential for organizing microtubule attachment sites. PMID: 15548592
    16. HsNUF2 and CENP-E are required for organization of stable microtubule-kinetochore attachment that is essential for faithful chromosome segregation in mitosis PMID: 17535814
    17. CDCA1 may therefore be an ideal tumor-associated antigen useful for the diagnosis and immunotherapy of these cancer PMID: 18770861
    18. alternative splicing variants, M-RIP, HYAL2, CDCA1, and MSMB genes showed differential expressions between cancer cells and corresponding normal tissues. PMID: 19081476
    19. Chromosome congression in HSET + hNuf2 co-depleted cells required the plus-end directed motor CENP-E , which has been implicated in the gliding of mono-oriented kinetochores alongside adjacent K-fibres. PMID: 19525938

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  • 亚细胞定位：
    Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. NDC80 is required for efficient kinetochore localization.
  • 蛋白家族：
    NUF2 family
  • 数据库链接：

    HGNC: 14621

    OMIM: 611772

    KEGG: hsa:83540

    STRING: 9606.ENSP00000271452

    UniGene: Hs.651950