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  4. Recombinant Human Kinetochore protein NDC80 homolog (NDC80)

Recombinant Human Kinetochore protein NDC80 homolog (NDC80)

  • 货号：
    CSB-YP015601HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015601HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015601HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015601HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015601HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NDC80
  • Uniprot No.：
  • 别名：
    from Entrez Gene ,; HEC; Highly expressed in cancer; Highly expressed in cancer protein; Highly expressed in cancer rich in leucine heptad repeats; HsHec1; hsNDC80; Kinetochore associated 2; Kinetochore associated protein 2; Kinetochore protein Hec1; Kinetochore protein NDC80 homolog; Kinetochore-associated protein 2; KNTC2; ndc80; NDC80 homolog kinetochore complex component; NDC80 kinetochore complex component homolog; NDC80, S. cerevisiae, homolog of; NDC80_HUMAN; Retinoblastoma associated protein HEC; Retinoblastoma-associated protein HEC; TID3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-642
  • 氨基酸序列
    MKRSSVSSGG AGRLSMQELR SQDVNKQGLY TPQTKEKPTF GKLSINKPTS ERKVSLFGKR TSGHGSRNSQ LGIFSSSEKI KDPRPLNDKA FIQQCIRQLC EFLTENGYAH NVSMKSLQAP SVKDFLKIFT FLYGFLCPSY ELPDTKFEEE VPRIFKDLGY PFALSKSSMY TVGAPHTWPH IVAALVWLID CIKIHTAMKE SSPLFDDGQP WGEETEDGIM HNKLFLDYTI KCYESFMSGA DSFDEMNAEL QSKLKDLFNV DAFKLESLEA KNRALNEQIA RLEQEREKEP NRLESLRKLK ASLQGDVQKY QAYMSNLESH SAILDQKLNG LNEEIARVEL ECETIKQENT RLQNIIDNQK YSVADIERIN HERNELQQTI NKLTKDLEAE QQKLWNEELK YARGKEAIET QLAEYHKLAR KLKLIPKGAE NSKGYDFEIK FNPEAGANCL VKYRAQVYVP LKELLNETEE EINKALNKKM GLEDTLEQLN AMITESKRSV RTLKEEVQKL DDLYQQKIKE AEEEDEKCAS ELESLEKHKH LLESTVNQGL SEAMNELDAV QREYQLVVQT TTEERRKVGN NLQRLLEMVA THVGSVEKHL EEQIAKVDRE YEECMSEDLS ENIKEIRDKY EKKATLIKSS EE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.
  • 基因功能参考文献：
    1. Aurora A kinase regulates kinetochore-microtubule dynamics of metaphase chromosomes, and Hec1 S69, a previously uncharacterized phosphorylation target site in the Hec1 tail, is a critical Aurora A substrate for this regulation. PMID: 29187526
    2. Ska complex directly binds Ndc80 complex through interactions between the Ska3 unstructured C-terminal region and the coiled-coil regions of each Ndc80 complex subunit. PMID: 29487209
    3. Hec1 tail phosphorylation tunes friction along polymerizing microtubules and yet does not compromise the kinetochore's ability to grip depolymerizing microtubules. PMID: 28552353
    4. We provided novel evidence that NDC80 expression is upregulated in osteosarcoma tissues, where it is positively correlated with advanced tumor stage and distant metastasis PMID: 28537682
    5. Ndc80 recruits Bod1 to kinetochores which directly feeds forward to regulate Ndc80 PMID: 29142109
    6. The s show that the Astrin-SKAP complex binds synergistically to microtubules with the Ndc80 complex to form an integrated interface. PMID: 28841134
    7. Elevated expression of NDC80 may play a role in promoting the development of hepatocellular carcinoma PMID: 28611520
    8. Study shows that Cdk1 phosphorylates Ska3 to promote its direct binding to the Ndc80 complex (Ndc80C), a core outer kinetochore component, also show that this phosphorylation occurs specifically during mitosis and is required for the kinetochore localization of the Ska complex. PMID: 28479321
    9. This study suggests that Ndc80 may play an important role in the process of hepatitis B virus-related hepatocellular carcinoma, and that it may be a potential biological treatment target in the future. PMID: 26382282
    10. Whereas CENP-C recruits a single MIS12:NDC80 complex, the s show here that CENP-T binds one MIS12:NDC80 and two NDC80 complexes upon phosphorylation by the mitotic CDK1:Cyclin B complex at three distinct CENP-T sites. PMID: 28012276
    11. Ska is recruited to kinetochores by clusters of Ndc80 proteins. PMID: 28535377
    12. Expression of NDC80 in colon cancer cells and tissues was higher than that in controls. NDC80 promotes the proliferation and metastasis of colon cancer cells. PMID: 27173328
    13. Ndc80 complex bound to microtubules binds every tubulin monomer along the microtubule protofilament. PMID: 26941333
    14. we conclude that Hec1 is consistently overexpressed in human PCa and Hec1 is closely linked with human PCa progression through the meditator LncRNA BX647187 PMID: 26612002
    15. Independent molecular binding events to microtubules (MTs) by individual NDC80 complexes, rather than their structured oligomers, regulate the dynamics and stability of kinetochore-MT attachments in dividing cells. PMID: 25808492
    16. The Ndc80 complex binds straight microtubules by recognizing the dimeric interface of tubulin. PMID: 24413531
    17. Overproduction of Ndc80 in cancer cells may unfavourably absorb protein interactors through the internal loop domain and lead to a change in the equilibrium of microtubule-associated proteins. [Review] PMID: 25557589
    18. N-terminus-modified Hec1 suppresses tumour growth by interfering with kinetochore-microtubule dynamics PMID: 25132262
    19. Mechanisms of mitosis-specific assembly of the checkpoint platform Knl1/MIS12/NDC80 at human kinetochores. PMID: 25601404
    20. Certain clinical subtypes of breast cancer more likely to respond to Hec1-targeted therapy were identified and these subtypes are the ones associated with poor prognosis. PMID: 24694948
    21. Hec1 is critical in maintaining the in vitro and in vivo growth of gastric cancer cells; elevated Hec1 levels may occur at the early stage of gastric tumorigenesis PMID: 23591767
    22. Growth inhibition following knockdown of NDC80, CDK1 and PLK1. PMID: 24327015
    23. Hec1 expression was highest in paclitaxel-resistant A2780/Taxol cells. PMID: 23474708
    24. these findings demonstrated that three buried glutamic acid-lysine pairs, in concert with hydrophobic interactions of core residues, provide the major specificity and stability requirements for Hec1-Nuf2 dimerization PMID: 24129578
    25. a novel role for Aurora B-Hec1-Mps1 signaling axis in governing accurate chromosome segregation in mitosis PMID: 24187132
    26. NDC80, NUF2 and PTN were significantly aberrantly overexpressed in serous adenocarcinomas. PMID: 23056589
    27. Ndc80's interaction with either growing or shrinking microtubule ends can be tuned by the phosphorylation state of its tail. PMID: 23085714
    28. The Ndc80 internal loop is essential for end-on microtubule attachment to kinetochores. PMID: 22454517
    29. The Ndc80 kinetochore complex directly modulates microtubule dynamics. PMID: 22908300
    30. results reveal the molecular function of CENP-T proteins and demonstrate how the Ndc80 complex is anchored to centromeres in a manner that couples chromosome movement to spindle dynamics PMID: 22561346
    31. results support the conclusion that Cdt1 binding to Hec1 is essential for an extended Ndc80 configuration and stable kinetochore-microtubule attachment PMID: 22581055
    32. Hec1 serine 165 (S165) is preferentially phosphorylated at kinetochores by Nek2 and it serves as an important mechanism in modulating spindle assemble checkpoint signaling and chromosome alignment. PMID: 21832156
    33. In vertebrates there is a tripartite attachment point facilitating the interaction between Hec1/Ndc80 and microtubules. PMID: 21325630
    34. N-terminally modified Hec1 promotes spindle pole fragmentation by CENP-E-mediated plus-end directed kinetochore pulling forces that disrupt the fine balance of kinetochore- and centrosome-associated forces regulating spindle bipolarity PMID: 21297979
    35. These data suggest that the CH and tail domains of Hec1 generate essential contacts between kinetochores and microtubules in cells, whereas the Nuf2 CH domain does not. PMID: 21270439
    36. Hec1 phosphorylation control kinetochore-microtubule attachment stability during mitosis. PMID: 21266467
    37. Alteration in NDC80, were also detected in benign breast tumors. PMID: 21352579
    38. Data provide evidence for a functional link between Hec1 expression and the pRb pathway. PMID: 20948316
    39. CENP-U is a novel microtubule binding protein and plays an important role in kinetochore-microtubule attachment through its interaction with Hec1 PMID: 21056971
    40. subnanometre-resolution cryo-electron microscopy reconstruction of the human Ndc80 complex bound to microtubules PMID: 20944740
    41. Hec 1 was highhy expressed in cancer tissues with lymph node metastasis and poor differentiation. PMID: 18782526
    42. Results suggest that Hec1, through cooperation with Hice1, contributes to centrosome-directed microtubule growth to facilitate establishing a proper mitotic spindle. PMID: 19776357
    43. cell growths of colorectal and gastric cancers after the siRNA-mediated knockdown of either CDCA1 or KNTC2 were significantly suppressed. PMID: 19878654
    44. role in spindle checkpoint signaling; required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores PMID: 12351790
    45. cell cycle-regulated serine phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation PMID: 12386167
    46. HEC1 is a core component of the kinetochore outer plate essential for organizing microtubule attachment sites. PMID: 15548592
    47. the Spc24, Spc25, Nuf2, and Ndc80/Hec1 complex is a faithful copy of the endogenous Ndc80 complex PMID: 15961401
    48. These findings reveal a key role for the Hec1 N terminus in controlling dynamic behavior of kinetochore microtubules. PMID: 17129782
    49. Study describes the crystal structure of the most conserved region of HEC1, which lies at one end of the rod and near the N terminus of the polypeptide chain, it folds into a calponin-homology domain. PMID: 17195848
    50. overexpression of either CREB or ATF4 enhanced the activation of the HEC1 promoter and overexpression of both of them had an additive effect on the activation of the HEC1 transcription. PMID: 17822787

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  • 亚细胞定位：
    Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
  • 蛋白家族：
    NDC80/HEC1 family
  • 数据库链接：

    HGNC: 16909

    OMIM: 607272

    KEGG: hsa:10403

    STRING: 9606.ENSP00000261597

    UniGene: Hs.414407