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  4. Recombinant Human Interferon-inducible protein AIM2 (AIM2)

Recombinant Human Interferon-inducible protein AIM2 (AIM2)

  • 货号：
    CSB-YP001499HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001499HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP001499HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001499HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001499HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Absent in melanoma 2; AIM 2; Aim2; AIM2_HUMAN; Interferon-inducible protein AIM2; OTTHUMP00000035296; PYHIN4
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-343
  • 氨基酸序列
    MESKYKEILL LTGLDNITDE ELDRFKFFLS DEFNIATGKL HTANRIQVAT LMIQNAGAVS AVMKTIRIFQ KLNYMLLAKR LQEEKEKVDK QYKSVTKPKP LSQAEMSPAA SAAIRNDVAK QRAAPKVSPH VKPEQKQMVA QQESIREGFQ KRCLPVMVLK AKKPFTFETQ EGKQEMFHAT VATEKEFFFV KVFNTLLKDK FIPKRIIIIA RYYRHSGFLE VNSASRVLDA ESDQKVNVPL NIIRKAGETP KINTLQTQPL GTIVNGLFVV QKVTEKKKNI LFDLSDNTGK MEVLGVRNED TMKCKEGDKV RLTFFTLSKN GEKLQLTSGV HSTIKVIKAK KKT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8. Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.
  • 基因功能参考文献：
    1. In humans with acute and chronic Hepatitis B exists activation of the AIM2 and IFI16 (in acute Hepatitis B), and the activation of the AIM2 and IFI16 can be inhibited by hepatitis B e antigen in chronic Hepatitis B. PMID: 30222506
    2. expression and activation of AIM2, and the increase in circulating mtDNA levels may contribute to the inflammatory process in patients with T2D. PMID: 29947343
    3. Caspase-1-dependent IL-1beta processing and secretion require the AIM2 inflammasome pathway in human dental pulp cells and the AIM2 inflammasome pathway is critical for regulation of the dental pulp immune response. PMID: 29178062
    4. rs855867 in AIM2 is not significantly associated with childhood autism spectrum disorder or the severity of the disease in a Chinese Han population. PMID: 29216786
    5. This study found IFI16 and AIM2 SNPs associated with higher levels of periodontal microorganisms and an increased percentage of periodontal disease clinical parameters. PMID: 28387608
    6. C-reactive protein polymorphisms and DNA methylation of the AIM2 gene influence associations between trauma exposure, posttraumatic stress disorder, and C-reactive protein. PMID: 28867284
    7. HBx-induced loss of AIM2 is associated with poor outcomes and facilitates hepatocellular carcinoma metastasis by triggering the epithelial-mesenchymal transition process. PMID: 28580773
    8. Clinicopathological analysis of colorectal cancer tissue revealed that low AIM2 expression was significantly associated with some clinicopathological features such as depth of invasion, TNM clinical stage and lymph node metastasis PMID: 28802238
    9. Human cytomegalovirus infection induces an AIM2 inflammasome response, which negatively influences viral life cycle. PMID: 28480966
    10. These results provide evidence for the role of AIM2 in the progression of cSCC and identify AIM2 inflammasome function as a potential therapeutic target in these invasive and metastatic tumors. PMID: 28526809
    11. RSAD2 and AIM2 Modulate Coxsackievirus A16 and Enterovirus A71 Replication in Neuronal Cells in Different Ways That May Be Associated with Their 5' Nontranslated Regions. PMID: 29263272
    12. AIM2 inflammasome expression and activation is increased early during the course of acute pancreatitis, and suggests that AIM2 activation may affect systemic inflammation and organ failure in these patients PMID: 28342645
    13. Overexpression of AIM2 in hepatocellular carcinoma (HCC) cells suppressed mammalian target of rapamycin (mTOR)-S6K1 pathway and further inhibited proliferation of HCC cells. PMID: 27167192
    14. AIM2 is an important component of the inflammasome in tegumentary leishmaniasis (TL) patients and is directly associated with the severity of lesions. PMID: 28397969
    15. AIM2 contributes to the maintenance of intestinal integrity via Akt and protects against Salmonella mucosal infection. PMID: 26838050
    16. study identifies the AIM2 inflammasome and cGAS/IFI16-STING-type I IFN pathway as a novel mechanism for host innate immunity to the ALVAC vaccine vector. PMID: 28947539
    17. Study conclude that functional AIM2 is preferentially expressed in adult human CD27+ B-cells, but is absent in cord blood mononuclear cells. PMID: 28809949
    18. results suggest that the function of AIM2 is focused on the innate immune response, and AIM2 is a detrimental host factor for influenza-induced lung injury and mortality. PMID: 28424239
    19. knockdown of AIM2 by small interfering RNA attenuated caspase-1 activation. the results suggest that EBV infection of human monocytes induces caspase-1-dependent IL-1beta production, and that AIM2, acting as an inflammasome, is involved in this response. PMID: 28369146
    20. These data indicate that human cytomegalovirus pUL83 interacts with human AIM2 in the cytoplasm during the early stages of virus infection. The pUL83/AIM2 interaction deregulates the activation of AIM2 inflammasome. PMID: 28219398
    21. The findings reveal an age-dependent reduction of AIM2 expression and activation which may explain reduced cytokine responses to the cytosolic DNA mimic poly(dA:dT) in healthy elderly individuals. Reduced AIM2-mediated sensing with age may contribute to increased vulnerability to bacterial or viral infections in the elderly. PMID: 26944367
    22. The extent of NLRC3 and AIM2 gene reduction was correlated with cancer progression. PMID: 26378020
    23. Molecular docking analysis reveals that the conformation switching may be important for the autoinhibition of the AIM2 protein. PMID: 27037024
    24. A significant upregulation of the PYHIN inflammasomes AIM2 and IFI16 in active inflammatory bowel disease versus controls was found. PMID: 26313692
    25. Lung tumor-associated plasmacytoid dendritic cells (pDCs) are responsive to the activation of AIM2 that promotes calcium efflux and reactive oxygen species from mitochondria, leading to calpain activation and high levels of IL-1alpha. PMID: 26506473
    26. analysis of a subset of inflammasome receptors including NLRP3, NLRC4 and AIM2 that triggers formation of the micrometer-sized spherical supramolecular complex called the ASC speck PMID: 26258904
    27. pyrin domain drives both filament formation and dsDNA binding PMID: 26197926
    28. activation of the AIM2 inflammasome did not require a priming signal in ascitic fluid-derived macrophages, demonstrating the preactivated state of the inflammasome in these cells. PMID: 25173967
    29. These results suggest that AIM2 expressed in human dental pulp plays an important role in the immune defense by activating the inflammasome signaling pathway. PMID: 24986444
    30. The comparison shows mouse AIM2 PYD domain adopts a unique alpha2-alpha3 helix conformation distinct from its human homologues, but similar to the pyrin domain of human NLRP10/PYNOD, which belongs to another family. PMID: 25888795
    31. Increased expression and activation of absent in melanoma 2 inflammasome components in lymphocytic infiltrates of abdominal aortic aneurysms PMID: 24618883
    32. EB1 has a role in linking AIM2 inflammasomes with autophagy-dependent secretion PMID: 25164813
    33. caspase-1, IL-1beta, and IL-18 are regulated by AIM2, which mediates inflammation-associated renal damage in hepatitis B virus-associated glomerulonephritis PMID: 24701032
    34. Lack of Absent in Melanoma 2 expression is associated with colorectal cancer. PMID: 24729378
    35. Thus, autophagy may function as a negative counter-regulatory mechanism for HMGB1-DNA complex-induced inflammasome activation, and provide a checkpoint to limit the development of inflammation. PMID: 24971542
    36. These results, together with the reported data on p202 HINb, lead to an interaction model for full-length p202 and dsDNA which provides a conceivable mechanism for the negative regulation of Aim2 inflammasome activation by p202. PMID: 24419611
    37. this study was to investigate the mRNA levels of AIM2 and ASC in a lymphocyte cell line (Jurkat) before and after MiR-143 introducing. PMID: 23811549
    38. Ethanol inhibits activation of NLRP3 and AIM2 inflammasomes in human macrophages--a novel anti-inflammatory action of alcohol. PMID: 24244322
    39. AIM2 mRNA levels were higher in benign prostate hyperplasia (BPH)than normal prostate; levels of AIM2 mRNA were lower in clinical tumor specimens; constitutive levels of AIM2 mRNA and protein were lower in a subset of prostate cancer cells compared with BPH cells; Implication that AIM2 inflammasome has a fundamental role in the generation of prostatic diseases PMID: 23864729
    40. Vascular endothelial/smooth muscle cells are able to respond to inflammatory signals by upregulation of AIM2 expression, indicating a role of AIM2 in vascular pathogenesis. PMID: 23790454
    41. Binding of Human papillomavirus 16 DNA leads to AIM2 inflammasome activation. PMID: 23764897
    42. AIM2 might act as an important DNA sensor and a potential biomarker for apopDNA-induced macrophage functional maturation and SLE disease PMID: 23479181
    43. Studies indicate that the HIN (hematopoietic expression, interferon-inducible nature, and nuclear localization) domains exhibit either absent in melanoma2 (Aim2) HIN-like or p202 HINa-like modes of DNA binding. PMID: 24164899
    44. Data suggest that AIM2 (absent in melanoma 2) expression is correlated with the immune clearance of hepatitis B virus (HBV) in the host. PMID: 23376086
    45. Novel structural features of the AIM2 pyrin domain and insights into the potential mechanisms of domain interactions important for AIM2 autoinhibition and inflammasome assembly. PMID: 23530044
    46. Our data highlight the dynamics of epidermal AIM2 expression, showing Langerhans cell and melanocyte-restricted expression in normal epidermis but a pronounced induction in subpopulations of epidermal keratinocytes under inflammatory conditions. PMID: 23171461
    47. End binding protein 1 directly interacted with AIM2 and ASC in vitro and in vivo. PMID: 22869553
    48. crystal structures of their HIN domains in complex with double-stranded (ds) DNA. PMID: 22483801
    49. expression of AIM2 and IFI16 may have oncogenic activities in the OSCC cells that have inactivated the p53 system. PMID: 22320325
    50. Human keratinocytes express a functional AIM2 inflammasome which triggers a strong IL-1beta release in response to cytosolic dsDNA. PMID: 22092578

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Activated inflammasomes can aggregate in the cytosol as speck-like particles.
  • 蛋白家族：
    HIN-200 family
  • 组织特异性：
    Expressed in spleen, small intestine, peripheral blood leukocytes, and testis.
  • 数据库链接：

    HGNC: 357

    OMIM: 604578

    KEGG: hsa:9447

    STRING: 9606.ENSP00000357112

    UniGene: Hs.733411