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  4. Recombinant Human guanylate-binding protein 1 (GBP1)

Recombinant Human guanylate-binding protein 1 (GBP1)

  • 货号：
    CSB-YP009297HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009297HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009297HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009297HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009297HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    GBP 1; GBP-1; GBP1; GBP1_HUMAN; GTP binding protein 1; GTP-binding protein 1; Guanine nucleotide binding protein 1; Guanine nucleotide-binding protein 1; Guanylate binding protein 1; Guanylate binding protein 1 interferon inducible 67kDa; Guanylate binding protein 1 interferon inducible; HuGBP 1; HuGBP-1; HuGBP1; Interferon induced guanylate binding protein 1; Interferon-induced guanylate-binding protein 1; OTTHUMP00000012352
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    1-589
  • 氨基酸序列
    MASEIHMTGP MCLIENTNGR LMANPEALKI LSAITQPMVV VAIVGLYRTG KSYLMNKLAG KKKGFSLGST VQSHTKGIWM WCVPHPKKPG HILVLLDTEG LGDVEKGDNQ NDSWIFALAV LLSSTFVYNS IGTINQQAMD QLYYVTELTH RIRSKSSPDE NENEVEDSAD FVSFFPDFVW TLRDFSLDLE ADGQPLTPDE YLTYSLKLKK GTSQKDETFN LPRLCIRKFF PKKKCFVFDR PVHRRKLAQL EKLQDEELDP EFVQQVADFC SYIFSNSKTK TLSGGIQVNG PRLESLVLTY VNAISSGDLP CMENAVLALA QIENSAAVQK AIAHYEQQMG QKVQLPTETL QELLDLHRDS EREAIEVFIR SSFKDVDHLF QKELAAQLEK KRDDFCKQNQ EASSDRCSAL LQVIFSPLEE EVKAGIYSKP GGYRLFVQKL QDLKKKYYEE PRKGIQAEEI LQTYLKSKES MTDAILQTDQ TLTEKEKEIE VERVKAESAQ ASAKMLQEMQ RKNEQMMEQK ERSYQEHLKQ LTEKMENDRV QLLKEQERTL ALKLQEQEQL LKEGFQKESR IMKNEIQDLQ TKMRRRKAC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promotes oxidative killing and delivers antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes.
  • 基因功能参考文献：
    1. we have found GBP1 was downregulated during osteogenic differentiation of hBM-MSCs. While knockdown of GBP1 promoted osteogenesis, overexpression of GBP1 suppressed osteogenesis of hBM-MSCs. PMID: 29348519
    2. Here, we show that the human protein GBP1 acts as a cytosolic "glue trap," capturing cytosolic Gram-negative bacteria through a unique protein motif and preventing disseminated infections in cell culture models. To escape from this GBP1-mediated host defense, Shigella employs a virulence factor that prevents or dislodges the association of GBP1 with cytosolic bacteria. PMID: 29233899
    3. Results show that GBP1 is overexpressed in triple-negative breast cancer (TNBC), under the control of EGFR and selectively affects the growth of TNBC cell lines. PMID: 29115931
    4. hGBP1F acts as a nucleotide-controlled molecular switch by modulating the accessibility of its farnesyl moiety, which does not require any supportive proteins PMID: 28645896
    5. These findings confirm the involvement of caspase-1 in non-classical secretion mechanisms and open novel perspectives for the extracellular function of secreted GBP-1. PMID: 28272793
    6. Results suggest that guanylate-binding protein 1 (GBP1) plays a pivotal role in anti-T. gondii protection of mesenchymal stromal cells (hMSCs) and may shed new light on clarifying the mechanism of host defense properties of hMSCs. PMID: 28123064
    7. Shigella flexneri infection induces rapid proteasomal degradation of human guanylate binding protein-1 (hGBP1); the mode of IpaH9.8 action highlights the functional importance of GBPs in antibacterial defenses PMID: 29144452
    8. Previously reported tetrameric and dimeric species of hGBP-1 and hGBP-5 were unmasked as dimers and monomers, respectively, with their shapes depending on both the bound nucleotide and the ionic strength of the solution. PMID: 28580591
    9. Taken together, these results provide a new understanding of the antiviral mechanism of human GBP1, which possesses potent anti-Kaposi's sarcoma-associated herpesvirus activity, and suggest the critical role of viral RTA in the evasion of the innate immune response during primary infection by Kaposi's sarcoma-associated herpesvirus. PMID: 28592529
    10. A novel role for hGBP1 in cell-autonomous immunity that is independent of pathogen-containing vacuole translocation. PMID: 26874079
    11. Elevated hGBP-1 RNA in ovarian tumors correlates with shorter recurrence-free survival. hGBP-1 does not confer paclitaxel resistance on MCF-7 and TMX2-28 breast cancer cells. PMID: 27590579
    12. the study not only highlights the importance of hGBP1 tetramer in stimulated GMP formation, but also demonstrates its role in the antiviral activity against hepatitis C virus. PMID: 27071416
    13. GBP1 expression is elevated in human Glioblastoma multiforme tumors and positively correlates with EGFRvIII status in Glioblastoma multiforme specimens, and its expression is inversely correlated with the survival rate of Glioblastoma multiforme patients. Taken together, these results reveal that GBP1 may serve as a potential therapeutic target for Glioblastoma multiforme with EGFRvIII mutation. PMID: 26848767
    14. GBP1 promotes lymph node metastasis and has a positive correlation with EGFR expression in esophageal squamous cell carcinoma. PMID: 26760981
    15. Molecular dynamics studies showed that only GTP decreases the formation of the GBP1:PIM1 complex through an allosteric mechanism, outlining the rational for the identification of new compounds potentially able to revert resistance to paclitaxel. PMID: 25081641
    16. GBP1 overexpression is necessary for the radioresistant phenotype in clinically relevant radioresistant cells PMID: 25098609
    17. data show that dimer formation of hGBP1 involves multiple spatially distant regions of the protein, namely, the N-terminal LG domain and the C-terminal helices alpha13. PMID: 24991938
    18. GBP-1 is a downstream processor of IFN-gamma. PMID: 24337748
    19. GBP-1 is a novel member within the family of actin-remodeling proteins specifically mediating IFN-gamma-dependent defense strategies. PMID: 24190970
    20. IFN-gamma-induced Guanylate Binding Protein-1 is a novel Actin Cytoskeleton Remodeling Factor. GBP-1 may induce actin remodeling via globular actin sequestering and/or filament capping. GBP-1 is a novel member within the family of actin remodeling proteins, specifically mediating IFN-gamma-dependent defense strategies. PMID: 24190970
    21. Data indicate that among three deductive p53 response element (p53RE) present in the hGBP1 promoter region, two p53REs were found to be transactivated by p53. PMID: 23727578
    22. GBP1/2 are critical effectors of antichlamydial interferon (IFN)gamma-mediated pathogen clearance via rerouting of bacterial inclusions in macrophages for lysosomal degradation. PMID: 23086406
    23. Thermodynamic insight as to how the stability of an intermediate catalytic complex regulates the product formation in hGBP1. PMID: 22859948
    24. Cytokine-induced GBP-1 retards cell proliferation by forming a negative feedback loop that suppresses beta-catenin / TCF signaling. PMID: 22692453
    25. results suggested that GBP-1 acts directly as a tumor suppressor in CRC and the loss of GBP-1 expression might indicate tumor evasion from the IFN-gamma-dominated Th1 immune response. PMID: 23042300
    26. GBP1 inhibits proliferation, migration, invasion and tumor formation of colon tumor cells. PMID: 23042300
    27. Data indicate that alpha12/13 represents a stable subdomain of guanylate-binding protein 1 (hGBP1). PMID: 22607347
    28. Data indicate that GBP1 guanine cap (i.e., C-terminal guanine-binding amino acid motif, particularly Arg240/Arg244) is key structural element responsible for dimerization and is essential for self-activation of GTPase activity. PMID: 22059445
    29. higher GBP1 level in oral cavity squamous cell carcinoma tissue was associated with higher overall pathological stage, positive perineural invasion, and poorer prognosis PMID: 21714544
    30. establish GBP1 as a previously unknown link between EGFR activity and MMP1 expression and nominate it as a novel potential therapeutic target for inhibiting GBM invasion. PMID: 22162832
    31. Data indicate that GBP-1 contributes to vascular dysfunction in chronic inflammatory diseases by inhibiting endothelial progenitor cell (EPC) angiogenic activity via the induction of premature EPC differentiation. PMID: 20716116
    32. GBP-1 cellular 1ocalization depends on prenylation and dimerization. PMID: 21151871
    33. hGBP-1, hGBP-2 showed dimerization-related GTPase activity for GMP formation. PMID: 20923658
    34. Animals carrying murine mammary carcinoma cells that had been given doxycycline for induction of human GBP-1 expression revealed a significantly reduced tumor growth compared with mock-treated mice. PMID: 20454519
    35. Results identify intramolecular contacts of guanylate binding protein 1, which relay nucleotide-dependent structural changes from the N-terminus to the C-terminus and thereby mediate tetramer formation of the protein. PMID: 20450919
    36. These findings identify a role for IFN-alphaA-mediated GBP-1 expression in the prevention of intestinal epithelial apoptosis by commensal bacteria. PMID: 20483731
    37. designed point mutants in the phosphate-binding loop (P-loop) as well as in the switch I and switch II regions. These mutant proteins were analysed for their interaction with guanine nucleotides and for their ability to hydrolyse GTP. PMID: 15504415
    38. Golgi targeting of human guanylate-binding protein-1 requires nucleotide binding, isoprenylation, and an IFN-gamma-inducible cofactor. PMID: 15937107
    39. GBP-1 regulates anti-proliferative effect of inflammatory cytokines. It also mediates inhibition of endothelial cell invasiveness by down regulation of MMP-1[review] PMID: 16005050
    40. crystal structures of the N-terminal G domain trapped at successive steps along the reaction pathway and biochemical data reveal the molecular basis for nucleotide-dependent homodimerization and cleavage of GTP PMID: 16511497
    41. kinetic investigation of GTP hydrolysis catalyzed by interferon-gamma-induced hGBP1 PMID: 16873363
    42. Interferon-alpha upregulates GBP1 in cultured human vascular endothelial cells. PMID: 16894355
    43. 3 genes were upregulated in patients with chronic EBV infection: guanylate binding protein 1, tumor necrosis factor-induced protein 6, and guanylate binding protein 5; they may be associated with the inflammatory reaction or with cell proliferation. PMID: 18260761
    44. GBP-1 may be a novel biomarker and an active component of a Th-1-like angiostatic immune reaction in colorectal carcinoma. PMID: 18697200
    45. GBP-1 is a novel marker of intestinal mucosal inflammation that may protect against epithelial apoptosis induced by inflammatory cytokines and subsequent loss of barrier function PMID: 19079332
    46. The s demonstrate for the first time that both the alpha-helix of the intermediate region and the (103)DXEKGD(108) motif play critical roles for the hydrolysis to GMP. PMID: 19150356
    47. The results indicate that the GBP1, STAT1 and CXCL10 may be novel risk genes for the differentiation of PBM at the monocyte stage. PMID: 19223260
    48. Positions of cysteine residues buried between the C-terminal domain of GBP1 and the rest of the protein are identified which report a large change of accessibility by the compound after addition of GTP. PMID: 19463820
    49. Inhibition of endothelial cell spreading and migration by inflammatory cytokines is mediated by GBP-1 through induction of ITGA4 expression. PMID: 18697840
    50. Human guanylate binding protein-1 may be a useful surrogate marker for diagnosis of bacterial meningitis PMID: 16936281

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  • 亚细胞定位：
    Cytoplasm. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Cell membrane. Secreted. Note=Secreted from endothelial cells in the cerebrospinal fluid, upon bacterial challenge and independently of IFNG induction. Golgi membrane localization requires isoprenylation and the presence of another IFNG-induced factor.
  • 蛋白家族：
    TRAFAC class dynamin-like GTPase superfamily, GB1/RHD3-type GTPase family, GB1 subfamily
  • 数据库链接：

    HGNC: 4182

    OMIM: 600411

    KEGG: hsa:2633

    STRING: 9606.ENSP00000359504

    UniGene: Hs.62661