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  4. Recombinant Human Inhibitor of nuclear factor kappa-B kinase subunit alpha (CHUK)

Recombinant Human Inhibitor of nuclear factor kappa-B kinase subunit alpha (CHUK)

  • 货号：
    CSB-YP005420HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005420HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005420HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005420HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005420HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    chuk; CHUK1; Conserved Helix Loop Helix Ubiquitous Kinase; Conserved helix loop ubiquitous kinase; Conserved helix-loop-helix ubiquitous kinase; I Kappa B Kinase 1; I Kappa B Kinase Alpha; I-kappa-B kinase 1; I-kappa-B kinase alpha; IkappaB kinase; IkB kinase alpha subunit; IkBKA; IKK 1; IKK A; IKK a kinase; IKK-A; IKK-alpha; IKK1; IKKA; IKKA_HUMAN; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells; Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit; Inhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; Nuclear Factor Kappa B Inhibitor Kinase Alpha; Nuclear factor NF kappa B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NFkappaB inhibitor kinase alpha; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor; TCF-16; TCF16; Transcription factor 16
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-745
  • 氨基酸序列
    MERPPGLRPG AGGPWEMRER LGTGGFGNVC LYQHRELDLK IAIKSCRLEL STKNRERWCH EIQIMKKLNH ANVVKACDVP EELNILIHDV PLLAMEYCSG GDLRKLLNKP ENCCGLKESQ ILSLLSDIGS GIRYLHENKI IHRDLKPENI VLQDVGGKII HKIIDLGYAK DVDQGSLCTS FVGTLQYLAP ELFENKPYTA TVDYWSFGTM VFECIAGYRP FLHHLQPFTW HEKIKKKDPK CIFACEEMSG EVRFSSHLPQ PNSLCSLVVE PMENWLQLML NWDPQQRGGP VDLTLKQPRC FVLMDHILNL KIVHILNMTS AKIISFLLPP DESLHSLQSR IERETGINTG SQELLSETGI SLDPRKPASQ CVLDGVRGCD SYMVYLFDKS KTVYEGPFAS RSLSDCVNYI VQDSKIQLPI IQLRKVWAEA VHYVSGLKED YSRLFQGQRA AMLSLLRYNA NLTKMKNTLI SASQQLKAKL EFFHKSIQLD LERYSEQMTY GISSEKMLKA WKEMEEKAIH YAEVGVIGYL EDQIMSLHAE IMELQKSPYG RRQGDLMESL EQRAIDLYKQ LKHRPSDHSY SDSTEMVKII VHTVQSQDRV LKELFGHLSK LLGCKQKIID LLPKVEVALS NIKEADNTVM FMQGKRQKEI WHLLKIACTQ SSARSLVGSS LEGAVTPQTS AWLPPTSAEH DHSLSCVVTP QDGETSAQMI EENLNCLGHL STIIHEANEE QGNSMMNLDW SWLTE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates AMBRA1 following mitophagy induction, promoting AMBRA1 interaction with ATG8 family proteins and its mitophagic activity.
  • 基因功能参考文献：
    1. evidence that DCNL5 may be involved in innate immunity, as it is a direct substrate of the kinase IKKalpha during immune signalling. PMID: 29958295
    2. cFLIP appears to bind to IKKalpha to prevent IKKalpha from phosphorylating and activating IRF7. PMID: 29222334
    3. functions as a suppressor of lung adenocarcinoma; deletion up-regulates NOX2 and down-regulates NRF2, leading to ROS accumulation and blockade of cell senescence induction PMID: 29311298
    4. HOTAIR operates the action of IKKalpha, IKKbeta, IKKgamma in liver cancer stem cells PMID: 27367027
    5. Results indicate the involvement of IKK and NF-kappaB signaling in the maintenance of glioblastoma stem cell. PMID: 27732951
    6. Loss-of-function of LINC00473 in vivo effectively promoted the regression of Wilms tumour via miR-195/IKKalpha-mediated growth inhibition. PMID: 29159834
    7. Study results provide new insights into the molecular mechanisms of maspin suppression in response to HBx, and revealed nuclear IKKalpha as a prognostic biomarker and a potential therapeutic target to improve the clinical outcome of HBV-associated HCC patients. PMID: 27409165
    8. Data show that IKKalpha directly bind to the promoters of LGR5, in turn, upregulating LGR5 expression through activation of STAT3 signaling pathway during cancer progression. PMID: 27049829
    9. single-particle cryoelectron microscopy (cryo-EM) and X-ray crystal structures of human IKK1 in dimeric ( approximately 150 kDa) and hexameric ( approximately 450 kDa) forms, are reported. PMID: 27851956
    10. results suggest that changes in the relative concentrations of RelB, NIK:IKK1, and p100 during noncanonical signaling modulate this transitional complex and are critical for maintaining the fine balance between the processing and protection of p100. PMID: 27678221
    11. IKKalpha-dependent phosphorylation of S376 stimulated whereas IKKalpha-independent phosphorylation of S484 inhibited RORgammat function in Th17 differentiation. PMID: 28667162
    12. IKKalpha promotes migration through dynamic interactions with the EGF promoter depending on the redox state within cells. PMID: 28122935
    13. the dual regulation of STAT1 by IKKalpha in antiviral signaling suggests a role for IKKalpha in the fine-tuning of antiviral signaling in response to non-self RNA. PMID: 27992555
    14. In epithelial ovarian cancer cells, miR-23a enhances the expression of IKKalpha. The proliferation, migration, and invasion of EOC cells are increased by IKKalpha. PMID: 27537390
    15. TLR signaling led to lower expression of LRRC14. PMID: 27426725
    16. IKKalpha is an important determinant of poor outcome in patients with ER-positive invasive ductal breast cancer and thus may represent a potential therapeutic target PMID: 28006839
    17. The molecular mechanisms involved in IKKalpha-related tumors. [review] PMID: 26323241
    18. this study shows that miR-23a regulated IL-17-mediated proinflammatory mediators expression in rheumatoid arthritis by directly targeting IKKalpha PMID: 27936459
    19. W346 effectively inhibited tumor necrosis factor (TNF-a)-induced NF-kappaB activation by suppressing IKK phosphorylation, inhibiting IkB-a degradation, and restraining the accumulation of NF-kappaB subunit p65 nuclear translocation. W346 also affected NF-kappaB-regulated downstream products involved in cycle arrest and apoptosis. PMID: 26520440
    20. Data indicate a significantly higher quantity of mesenchymal stromal cells (MSCs)was produced from human embryonic stem cells (hESCs) with IkappaB kinase (IKK)/nuclear factor kappa B (NF-kappaB) suppression. PMID: 26972683
    21. Findings indicate that IkappaB kinase inhibitor ACHP can slowdown the accumulation of collagen type I (COL1A1). PMID: 26337045
    22. TRIM22 could interact with IkappaB kinase (IKK)alpha but not IKKbeta and could increase the level and phosphorylation of IKKalpha through its really interesting new gene (RING) and spla-ryanodine receptor (SPRY) domains. PMID: 25510414
    23. IKKalpha is diversely expressed in keratinizing and non-keratinizing carcinomas in the same type of cancer. PMID: 26317791
    24. results indicate that nuclear active IKK is a robust biomarker to predict cutaneous squamous cell carcinoma outcome, and suggest the possibility of targeting IKK activity as a future therapy for treating metastatic cutaneous squamous cell carcinomas PMID: 26094020
    25. We also report that the extra-genomic effects elicited by both ligands, leading to recruitment of active Akt to chromatin, are essential for phosphorylation of serine 10 in histone H3 by IKKa PMID: 25482200
    26. Upon infection, the HCV 3'UTR redistributes DDX3X and IKK-alpha to speckle-like cytoplasmic structures shown to be stress granules. PMID: 25740981
    27. Suppression of PKK expression by RNA interference inhibits phosphorylation of IKKalpha and IKKbeta as well as activation of NF-kappaB in human cancer cell lines; thus, PKK regulates NF-kappaB activation by modulating activation of IKKalpha and IKKbeta. PMID: 25096806
    28. Silencing of IKKa in E2-challenged cells resulted into an increased presence of either 8-oxo-Gs as well as of the base excision repair enzyme 8-oxo-guanine-DNA glycosylase 1. PMID: 24971480
    29. Findings indicated that IKKalpha plays a crucial role as a tumor suppressor that suppresses the invasion, metastasis, and angiogenesis of nasopharyngeal carcinoma (NPC) cells in vitro and correlates with the survival in NPC patients. PMID: 24753359
    30. s show that activation of NF-kappaB by Kaposi's sarcoma-associated herpesvirus K15 protein involves the recruitment of NF-kappaB-inducing kinase (NIK) and IKK alpha/beta to result in the phosphorylation of p65/RelA on Ser536. PMID: 25187543
    31. Data show that downstream of Akt protein, IkappaB kinase alpha (IKKalpha) directly phosphorylates mammalian target of rapamycin (mTOR) to drive mTORC1 activation. PMID: 24990947
    32. Survivin-2B promoted autophagy and further regulated cell death by accumulating and stabilizing IKK alpha in the nucleus of selenite treated leukemia cells. PMID: 24556686
    33. IKK complex functions as a key mediator of detachment-induced autophagy and anoikis resistance in epithelial cells. PMID: 23778976
    34. Increases in Ikappa B kinase alpha suppresses the progression of nasopharyngeal carcinoma. PMID: 24075781
    35. That block IKKalpha/beta and EGFR pathways. PMID: 23455325
    36. Data indicate that muscle IKK-alpha protein content was significantly lower in chronic obstructive pulmonary disease (COPD) patients. PMID: 24215713
    37. IKK interacts with rictor and regulates the function of mTORC2 including phosphorylation of AKT (at Serine473) and organization of actin cytoskeleton. PMID: 23872070
    38. roles for p53 and IKKalpha/IKKbeta in non-canonical Notch signaling and IL-6 as a novel non-canonical Notch target gene PMID: 23178494
    39. Data indicate that eight of twelve compounds showed acceptable inhibitory effects on IKKbeta. PMID: 23501112
    40. Results suggest that 13-197 targets IKKbeta and thereby inhibits mTOR and NF-kappaB pathways. PMID: 23444213
    41. Our findings suggest ERLIN1-CHUK-CWF19L1 variants are associated with early stage of fatty liver accumulation to hepatic inflammation. PMID: 23477746
    42. Results demonstrate that IL-8 expression is mediated, at least partly, by IKKalpha PMID: 23894194
    43. Reconstituting irradiated mutant animals with wild-type bone marrow (BM) prevented SCC development, suggesting that BM-derived IKKalpha mutant macrophages promote the transition of IKKalpha(low)K5(+)p63(hi) cells to tumor cells. PMID: 23597566
    44. This review highlights major advances in the studies of the nuclear functions of IKKalpha and the mechanisms of IKKalpha nuclear translocation. Understanding the nuclear activity is essential for targeting IKKalpha for therapeutics. PMID: 23343355
    45. Chemical inhibitors of IKK-alpha suppress HCV infection and IKK-alpha-induced lipogenesis, offering a proof-of-concept approach for new HCV therapeutic development PMID: 23708292
    46. Inhibition of IKKalpha partially rescued p53 levels, while concomitant IKKalpha inhibition fully rescued p53 and regulates MDM2 SUMOylation. PMID: 23032264
    47. H5N1 virus NS1 not only blocks IKKbeta-mediated phosphorylation and degradation of IkappaBalpha in the classical pathway but also suppresses IKKalpha-mediated processing of p100 to p52 in the alternative pathway. PMID: 22891964
    48. The results suggest that inactivation of IKKalpha, followed by Akt and FOXO1 phosphorylation and caspase-3 activation, contributes to zerumbone-induced GBM cell apoptosis. PMID: 23035900
    49. Active nuclear p45-IKKalpha forms a complex with nonactive IKKalpha and NEMO that mediates phosphorylation of SMRT and histone H3. PMID: 23041317
    50. E7 proteins from the cutaneous human papillomavirus types demonstrated interaction with IKKalpha but not with IKKbeta. PMID: 22776252

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  • 相关疾病：
    Cocoon syndrome (COCOS)
  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, I-kappa-B kinase subfamily
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 1974

    OMIM: 600664

    KEGG: hsa:1147

    STRING: 9606.ENSP00000359424

    UniGene: Hs.198998