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  4. Recombinant Human Hypermethylated in cancer 1 protein (HIC1)

Recombinant Human Hypermethylated in cancer 1 protein (HIC1)

  • 货号：
    CSB-YP623921HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP623921HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP623921HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP623921HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP623921HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HIC1
  • Uniprot No.：
  • 别名：
    Hic 1 ; HIC ZBTB transcriptional repressor 1; Hic-1; Hic1; HIC1_HUMAN; Hypermethylated in cancer 1; Hypermethylated in cancer 1 protein; ZBTB29; Zinc finger and BTB domain-containing protein 29; ZNF901
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-733
  • 氨基酸序列
    MTFPEADILL KSGECAGQTM LDTMEAPGHS RQLLLQLNNQ RTKGFLCDVI IVVQNALFRA HKNVLAASSA YLKSLVVHDN LLNLDHDMVS PAVFRLVLDF IYTGRLADGA EAAAAAAVAP GAEPSLGAVL AAASYLQIPD LVALCKKRLK RHGKYCHLRG GGGGGGGYAP YGRPGRGLRA ATPVIQACYP SPVGPPPPPA AEPPSGPEAA VNTHCAELYA SGPGPAAALC ASERRCSPLC GLDLSKKSPP GSAAPERPLA ERELPPRPDS PPSAGPAAYK EPPLALPSLP PLPFQKLEEA APPSDPFRGG SGSPGPEPPG RPDGPSLLYR WMKHEPGLGS YGDELGRERG SPSERCEERG GDAAVSPGGP PLGLAPPPRY PGSLDGPGAG GDGDDYKSSS EETGSSEDPS PPGGHLEGYP CPHLAYGEPE SFGDNLYVCI PCGKGFPSSE QLNAHVEAHV EEEEALYGRA EAAEVAAGAA GLGPPFGGGG DKVAGAPGGL GELLRPYRCA SCDKSYKDPA TLRQHEKTHW LTRPYPCTIC GKKFTQRGTM TRHMRSHLGL KPFACDACGM RFTRQYRLTE HMRIHSGEKP YECQVCGGKF AQQRNLISHM KMHAVGGAAG AAGALAGLGG LPGVPGPDGK GKLDFPEGVF AVARLTAEQL SLKQQDKAAA AELLAQTTHF LHDPKVALES LYPLAKFTAE LGLSPDKAAE VLSQGAHLAA GPDGRTIDRF SPT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. Involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF-responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription of ACKR3, FGFBP1 and EFNA1.
  • 基因功能参考文献：
    1. In this present work, the s identify and characterize a transcription factor i.e. HIC1, which physically interacts with both Bcl11b/CTIP2 and HMGA1 to co-regulate specific subsets of cellular genes and the HIV-1 tat gene. PMID: 27725726
    2. our data demonstrate that HIC1 SUMOylation is important for the transcriptional response to non-repairable DSBs but dispensable for DNA repair. PMID: 27935866
    3. Data suggest that aberrant expression of hypermethylated in cancer-1 (HIC1)/sirtuin-1 (SIRT1) and hypermethylation of the HIC1 promoter may be critical for the development and progression of papillary thyroid cancer. PMID: 27793057
    4. Hypermethylation of HIC1 gene is associated with Colon Cancer. PMID: 26671036
    5. Data show that secretion of IL-6 induced by loss of HIC1 activated STAT3 through IL-6/JAK pathway and was associated with NSCLC progression. PMID: 27107418
    6. The VNTR sequence near HIC1 could be a predictive marker for oxaliplatin-based chemotherapy in patients with metastatic colorectal cancer. PMID: 28708932
    7. Therefore, our data identify HIC1 as a novel factor involved in B cell differentiation acting as an epigenetic repressor of CIITA transcription. PMID: 27720955
    8. HIC1 loss promotes prostate cancer metastasis by triggering epithelial-mesenchymal transition. PMID: 28466555
    9. Data show that expression of hypermethylated in cancer 1 protein (HIC1) is downregulated in uveal melanoma. PMID: 27449031
    10. HIC1 attenuates invasion and metastasis by inhibiting the IL-6/STAT3 signalling pathway in human pancreatic ductal adenocarcinoma. PMID: 27085461
    11. Epigenetic silencing of HIC1 promotes epithelial-mesenchymal transition and drives progression in esophageal squamous cell carcinoma via EphA2 signaling. PMID: 26510908
    12. The tumor-suppressive function of Hic1 in colon is related to its inhibitory action on proproliferative signaling mediated by the Tlr2 receptor present on tumor cells. PMID: 25934696
    13. Results demonstrated an important role of HIC1 for the normal progression of cell cycle, and could affect the homeostasis of p53 as well as number of cell cycle-related genes, which may or may not be directly linked to p53. PMID: 24992983
    14. We found that EVI1 and HIC1 colocalize in the nucleus, and their interaction is mediated by the amino terminal zinc finger binding domain of EVI1 PMID: 24907396
    15. HIC-1 expression was assessed on a tissue microarray containing 80 cases of breast cancer. PMID: 24489730
    16. Hypermethylation of HIC1 promoter and aberrant expression of HIC1/SIRT1 might contribute to the carcinogenesis of pancreatic cancer. PMID: 22552606
    17. ectopic expression of HIC1 in U2OS and MDA-MB-231 cell lines decreases expression of the ApoER2 and VLDLR genes, encoding two canonical tyrosine kinase receptors for Reelin. PMID: 24076391
    18. HIC1 silencing in triple-negative breast cancer drives progression through misregulation of LCN2. PMID: 24295734
    19. HIC1 interacts with and modulates the transcriptional activity of STAT3. PMID: 24067369
    20. Reactivation of HIC1 suppressed cell migration and induced cell cycle arrest in the G0/G1 phase, as well as induced apoptosis in gastric cancer cells. PMID: 23769968
    21. Our data show for the first time that hypermethylation of HIC1 promoter results in loss of its repressive function, responsible for prostate cancer progression and invasion. PMID: 23340301
    22. epigenetic HIC1 inactivation, which is an early step in tumorigenesis, could contribute to the accumulation of DNA mutations through impaired DNA repair and thus favor tumorigenesis. PMID: 23417673
    23. these results further demonstrate that HIC1 is a key player in the regulation of the DNA damage response. PMID: 23178572
    24. A growth-regulatory role of HIC1 in the parathyroid glands and perturbed expression of HIC1 may represent an early event during tumor development. PMID: 22544915
    25. Promoter hypermethylation of tumor suppressor HIC1 is associated with prostate carcinoma. PMID: 22136354
    26. these results demonstrate that the activating acetylation to SUMOylation switch of HIC1 is favored by genotoxic stresses to regulate the DNA damage response. PMID: 22510409
    27. Identify HIC1 hypermethylation in renal cell carcinoma as an independent predictor of reduced recurrence-free survival. PMID: 22327210
    28. identify HIC1 as the first transcription factor in mammals able to recruit PRC2 to some target promoters through its interaction with Polycomb-like proteins. PMID: 22315224
    29. loss of the regulation of this Eph pathway through HIC1 epigenetic silencing could be an important mechanism in the pathogenesis of epithelial cancers PMID: 22184117
    30. early inactivation of HIC1 in breast carcinomas could predispose to stress-induced metastasis through up-regulation of the beta-2 adrenergic receptor PMID: 22194601
    31. Silencing of HIC1 and TOB1 expression is a common occurrence in gastric cancer and may contribute to the development and progression of the disease. PMID: 21533545
    32. High frequency of methylation at MGMT, RASSFA, and HIC-1 was detected in colorectal carcinoma patients PMID: 21274674
    33. This review highlights the role of HIC1 inactivation in solid tumours and particularly in leukaemia development PMID: 21104471
    34. Maternal genes FLNB, HIC1 and ZNF189 were strongly associated with risk of clefting. PMID: 20634891
    35. Investigated the methylation of the SFRP2, P16, DAPK1, HIC1, and MGMT genes, as well as the mutation of amino acid codons 12 and 13 of the KRAS gene in normal and tumor tissue DNA of patients diagnosed with sporadic colorectal cancer. PMID: 20682398
    36. identify MTA1, a subunit of the NuRD complex, as a new HIC1 corepressor PMID: 20547755
    37. The human candidate tumor suppressor gene HIC1 recruits CtBP through a degenerate GLDLSKK motif. PMID: 12052894
    38. aberrant methylation of HIC1 may play a role in the pathogenesis of specific pediatric tumors PMID: 14506157
    39. epigenetic silencing of HIC-1 may well contribute to the pathogenesis in the majority of medulloblastomas. PMID: 14656076
    40. Inactivation of HIC1 by hypermethylation is associated with medulloblastoma pathogenesis PMID: 14688019
    41. analysis of HIC1 consensus binding sequence and its DNA binding and repressive properties PMID: 15231840
    42. In human osteosarcomas, hypermethylation of HIC1 is frequent only in tumors with p53 mutation PMID: 15488761
    43. HIC1 p53-responsive element (HIC1.PRE) is necessary and sufficient to mediate induction of transcription by p53 PMID: 16301995
    44. HIC1 is involved in a certain feedback regulation for p53 in tumor suppression through histone deacetylase. (review) PMID: 16386221
    45. These data indicate that the intracellular amounts of HIC1 protein can modulate the level of the transcriptional stimulation of the genes regulated by canonical Wnt/beta-catenin signaling. PMID: 16724116
    46. HIC1 interaction with the corepressor CtBP depends on a central leucine residue PMID: 16762039
    47. HIC1 is a target of the class III deacetylase SIRT1 and identify a new posttranslational modification step in the P53-HIC1-SIRT1 regulatory loop. PMID: 17283066
    48. Detailed mapping of chromosome 17p deletions reveals HIC1 as a novel tumor suppressor gene candidate telomeric to TP53 in diffuse large B-cell lymphoma PMID: 17982487
    49. ARID1A/BAF250A was identified as a new HIC1 partner. PMID: 19486893
    50. Transcription factors were screened for regulation of a human HIC1 promoter reporter. Found that E2F1 strongly activates the full-length HIC1 promoter reporter. PMID: 19491197

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Krueppel C2H2-type zinc-finger protein family, Hic subfamily
  • 组织特异性：
    Ubiquitously expressed with highest levels found in lung, colon, prostate, thymus, testis and ovary. Expression is absent or decreased in many tumor cells.
  • 数据库链接：

    HGNC: 4909

    OMIM: 603825

    KEGG: hsa:3090

    STRING: 9606.ENSP00000314080

    UniGene: Hs.695682