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  4. Recombinant Human Hsp90 co-chaperone Cdc37 (CDC37)

Recombinant Human Hsp90 co-chaperone Cdc37 (CDC37)

  • 货号：
    CSB-YP613684HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP613684HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP613684HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP613684HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP613684HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CDC 37; Cdc37; CDC37 cell division cycle 37 homolog; CDC37 cell division cycle 37 S cerevisiae homolog; CDC37 cell division cycle 37; S cerevisiae; homolog of; Cdc37 homolog; CDC37 protein; CDC37_HUMAN; CDC37A; cell division cycle 37; Cell division cycle 37 homolog; Hsp90 chaperone protein kinase targeting subunit; Hsp90 chaperone protein kinase targeting subunit p50Cdc37; Hsp90 chaperone protein kinase-targeting subunit; Hsp90 co chaperone Cdc37; Hsp90 co-chaperone Cdc37; p50; p50Cdc37; S cerevisiae hypothetical protein CDC37
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-378
  • 氨基酸序列
    MVDYSVWDHI EVSDDEDETH PNIDTASLFR WRHQARVERM EQFQKEKEEL DRGCRECKRK VAECQRKLKE LEVAEGGKAE LERLQAEAQQ LRKEERSWEQ KLEEMRKKEK SMPWNVDTLS KDGFSKSMVN TKPEKTEEDS EEVREQKHKT FVEKYEKQIK HFGMLRRWDD SQKYLSDNVH LVCEETANYL VIWCIDLEVE EKCALMEQVA HQTIVMQFIL ELAKSLKVDP RACFRQFFTK IKTADRQYME GFNDELEAFK ERVRGRAKLR IEKAMKEYEE EERKKRLGPG GLDPVEVYES LPEELQKCFD VKDVQMLQDA ISKMDPTDAK YHMQRCIDSG LWVPNSKASE AKEGEEAGPG DPLLEAVPKT GDEKDVSV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. Inhibits HSP90AA1 ATPase activity.
  • 基因功能参考文献：
    1. Study showed that Cdc37 gene was up-regulated in human colorectal adenocarcinoma (CRC). Furthermore, knockdown of Cdc37 effectively reduced cell proliferation activity, enhanced apoptosis, and inhibited G1-S transition in CRC cells, and vice versa. For the mechanism, Cdc37 increased CDK4 stability to promote the phosphorylation of RB1, which finally promoted the progression of CRC. PMID: 29288563
    2. During the kinase chaperone cycle, Cdc37 phosphorylated at Y298 acts as a platform for docking of non-receptor tyrosine kinases through their regulatory domains to drive the coupled Hsp90 phosphorylation at Y197 and specifically regulate kinase chaperoning. PMID: 29343704
    3. findings suggested that this mechanism may be exploited by the Hsp90-Cdc37 chaperone to recruit and protect intrinsically dynamic kinase clients from degradation PMID: 29267381
    4. The results suggest a re-evaluation of the role of Cdc37 in the kinase lifecycle, and suggest that such interactions potentially allow kinases to more rapidly respond to key signals while simultaneously protecting unstable kinases from degradation and suppressing unwanted basal activity. PMID: 28784328
    5. Niclosamide ethanolamine disrupted the interaction between cell division cycle 37 and heat shock protein 90 in hepatocellular carcinoma, reducing tumor growth. PMID: 28284560
    6. Cdc37 performs a quality control of protein kinases, including b-raf, where induced conformational instability acts as a "flag" for Hsp90 dependence and stable cochaperone association. PMID: 27105117
    7. Ulk1 promoted the degradation of Hsp90-Cdc37 client kinases, resulting in increased cellular sensitivity to Hsp90 inhibitors. Thus, our study provides evidence for an anti-proliferative role of Ulk1 in response to Hsp90 inhibition in cancer cells PMID: 28073914
    8. The s find that the interaction between sB-Raf and the Hsp90 chaperone system is based on contacts with the M domain of Hsp90, which contributes in forming the ternary complex with Cdc37 as long as the kinase is not stabilized by nucleotide. PMID: 27620500
    9. Apart from these distinct Cdc37/Hsp90 interfaces, binding of the B-Raf protein kinase to the cochaperone is conserved between mammals and nematodes. PMID: 26511315
    10. Suppressing expression of the cochaperone CDC37 in hepatocellular carcinoma cells inhibits cell cycle progression and cell growth. PMID: 25098386
    11. Correlation between PDZK1, Cdc37, Akt and breast cancer malignancy: the role of PDZK1 in cell growth through Akt stabilization by increasing and interacting with Cdc37 PMID: 24869908
    12. The N-terminal tail serves as an intramolecular chaperone ensuring that CDC37 assumes one of two interconvertible states in a manner impacting the interaction of the client binding N-domain and the MC-domains, involved in dimerization and HSP90 binding. PMID: 25619116
    13. CDC37 has an important role in chaperoning protein kinases; it stabilizes kinase clients by a mechanism that is not dependent on a substantial direct interaction between CDC37 and HSP90, but requires HSP90 activity PMID: 24292678
    14. As a novel Hsp90 inhibitor, FW-04-806 binds to the N-terminal of Hsp90 and inhibits Hsp90/Cdc37 interaction, resulting in the disassociation of Hsp90/Cdc37/client complexes and the degradation of Hsp90 client proteins. PMID: 24927996
    15. CDC37 is a crucial HSP90-cofactor for KIT oncogenic expression in gastrointestinal stromal tumors PMID: 23584476
    16. SGK3 stability and kinase activation are regulated by the Hsp90-Cdc37 chaperone complex. PMID: 24379398
    17. Cdc37 (cell division cycle 37) restricts Hsp90 (heat shock protein 90) motility by interaction with N-terminal and middle domain binding sites. PMID: 23569206
    18. ERK5 interacts with the Hsp90-Cdc37 chaperone in resting cells, and inhibition of Hsp90 or Cdc37 results in ERK5 ubiquitylation and proteasomal degradation. PMID: 23428871
    19. an essential role for surface Cdc37 in concert with HSP90 on the cell surface during cancer cell invasion processes PMID: 22912728
    20. A series of tyrosine phosphorylation events, involving both p50(Cdc37) and Hsp90, are minimally sufficient to provide directionality to the chaperone cycle. PMID: 22727666
    21. Data show that part of the normal clearance cascade for TDP-43 involves the Cdc37/Hsp90 complex. PMID: 22674575
    22. Cdc37-mediated direct interaction between Hsp90/Cdc37 and an IRE1alpha cytosolic motif is important to maintain basal IRE1alpha activity and contributes to normal protein homeostasis and unfolded protein response under physiological stimulation. PMID: 22199355
    23. The primary mechanisms by which apigenin kill multiple myeloma cells is by targeting the trinity of CK2-Cdc37-Hsp90. PMID: 21871133
    24. the Hsp90 kinase co-chaperone Cdc37 regulates tau stability and phosphorylation dynamics PMID: 21367866
    25. Hsp90-Cdc37 complex acta as an endogenous regulator of noncanonical p38alpha activity. PMID: 20299663
    26. Tnf-induced recruitment and activation of the IKK complex require Cdc37 and Hsp90. PMID: 11864612
    27. role in regulating Hsp90 ATPase activity PMID: 11916974
    28. CDC37 binds to Akt and HSP90 in the signal transduction pathway in human tumor cells PMID: 12176997
    29. Results show that Cdc37 and heat shock protein 90 bind specifically to the kinase domain of LKB1. PMID: 12489981
    30. phosphorylation of Cdc37 on Ser13 is critical for its ability to coordinate Hsp90 nucleotide-mediated conformational switching and kinase binding PMID: 12930845
    31. Heteromeric comlpexes containing the molecular chaperones Hsp90 and Cdc37/p50 interacts with the kinase domain of LKB1. PMID: 14668798
    32. the interaction of Cdc37 with its client protein kinases requires amino acid residues within a motif that is present in many protein kinases PMID: 14701845
    33. Hsp90/p50cdc37 is required for mixed-lineage kinase (MLK) 3 signaling PMID: 15001580
    34. the Hsp90.Cdc37 molecular chaperone module has a central role in interleukin-1 receptor-associated-kinase-dependent signaling by toll-like receptors PMID: 15647277
    35. Cdc37 is found to heterodimerize with heat-shock protein 90 (Hsp90)-associating relative of Cdc37 (Harc) in vitro. PMID: 15850399
    36. Nuclear magnetic resonance study of binding to to HSP90. PMID: 16132836
    37. results suggest that a region of Cdc37 other than the client-binding site may be responsible for discriminating client protein kinases from others PMID: 16156789
    38. JAK1/2 are client proteins of Hsp90 alpha and beta; Hsp90 and CDC37 play a critical role in types I and II interferon pathways PMID: 16280321
    39. N-terminal glycine-rich loop of protein kinases is essential for physically associating with Cdc37. PMID: 16611982
    40. The data shows the expression and purification of an Hsp90-Cdc37-Cdk4 complex, defining its stoichiometry, and determining its 3D structure by single-particle electron microscopy. PMID: 16949366
    41. these observations support the hypothesis that there is a specific coordination between the activation of the cytosolic Ah receptor and the c-Src- and cdc37-containing HSP90 complex. PMID: 17223712
    42. The present data denote Hsp90-Cdc37 as a transiently acting essential regulatory component of IKK signaling. PMID: 17728246
    43. identify Pink1 as a novel Cdc37/Hsp90 client kinase PMID: 18003639
    44. Cdc37 is essential for maintaining prostate tumor cell growth and may represent a novel target in the search for multitargeted therapies. PMID: 18089825
    45. These data reveal a cyclic regulatory mechanism for Cdc37, in which its constitutive phosphorylation is reversed by targeted dephosphorylation in Hsp90 complexes. PMID: 18922470
    46. CDC37 in concert with HSP90 plays an essential role in maintaining oncogenic protein kinase clients including ERBB2, CRAF, CDK4, CDK6, & phosphorylated AKT. PMID: 18931700
    47. The human Cdc37.Hsp90 complex studied by heteronuclear NMR spectroscopy. PMID: 19073599
    48. C-terminal tail and determinants in the alphaE-helix of the catalytic domain allows the chaperones Hsp90 and Cdc37 to bind newly synthesized PKC beta II, a required event in the processing of PKC by phosphorylation PMID: 19091746
    49. celastrol may represent a new class of Hsp90 inhibitor by modifying Hsp90 C terminus to allosterically regulate its chaperone activity and disrupt Hsp90-Cdc37 complex. PMID: 19858214

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    CDC37 family
  • 数据库链接：

    HGNC: 1735

    OMIM: 605065

    KEGG: hsa:11140

    STRING: 9606.ENSP00000222005

    UniGene: Hs.160958