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  4. Recombinant Human Homeobox protein TGIF1 (TGIF1)

Recombinant Human Homeobox protein TGIF1 (TGIF1)

  • 货号：
    CSB-YP623005HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP623005HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP623005HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP623005HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP623005HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    5' TG 3' interacting factor; 5''-TG-3''-interacting factor 1; Homeobox protein TGIF ; Homeobox protein TGIF1; HPE4; MGC39747; MGC5066; TALE homeobox TG-interacting factor; TG interacting factor isoform c; TG-interacting factor; TGFB induced factor ; TGFB-induced factor homeobox 1; TGIF1; TGIF1_HUMAN; transforming growth factor-beta-induced factor
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-401
  • 氨基酸序列
    MVLAQSRVSA GVGSPHCSGS GGGGSDSFPW PASHPGNPQC SFSTAFLASP RLSRGTLAYL PPAPWSSLAT PSALLGSSCA PPPPPARCPQ PRALSPELGT KAGPRRPHRW ELPRSPSQGA QGPAPRRRLL ETMKGIVAAS GSETEDEDSM DIPLDLSSSA GSGKRRRRGN LPKESVQILR DWLYEHRYNA YPSEQEKALL SQQTHLSTLQ VCNWFINARR RLLPDMLRKD GKDPNQFTIS RRGAKISETS SVESVMGIKN FMPALEETPF HSCTAGPNPT LGRPLSPKPS SPGSVLARPS VICHTTVTAL KDVPFSLCQS VGVGQNTDIQ QIAAKNFTDT SLMYPEDTCK SGPSTNTQSG LFNTPPPTPP DLNQDFSGFQ LLVDVALKRA AEMELQAKLT A
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities.
  • 基因功能参考文献：
    1. Results suggest that silencing TGIF inhibited migration, invasion and metastasis of the human breast cancer cell line of MDAMB231 in vitro and in vivo. PMID: 29207164
    2. Data suggest that expression of TGIF is up-regulated by carcinogen BaP [benzo(a)pyrene]; this process is involved in BaP-induced cell proliferation, cell migration, tumor invasiveness, and tumor metastasis of lung adenocarcinoma cells. PMID: 29746904
    3. Based on the analysis of the TGIF1-homeodomain (HD) NMR structure determined here, the roles of two holoprosencephaly-related residues P192 and R219 in sufficient folding of TGIF1-HD were revealed. Mutations of these two residues, P192A and R219C found in holoprosence patients previously, resulted in structural change and less folding of TGIF1-HD, and thereby severely impaired the DNA-binding affinity of TGIF1-HD. PMID: 29355528
    4. High TGIF-1 expression is associated with fetal growth restriction. PMID: 27539603
    5. Tgifs regulate ciliogenesis and suggests that Evi5l mediates at least part of this effect. PMID: 27956704
    6. Knockdown of TGIF1 resulted in decreased protein expression of HOXD10 and increased resistance to colistin cytotoxicity in renal cells. PMID: 28335481
    7. Data show that the silencing of TG-interacting factor (TGIF) inhibited A549 lung cancer cell proliferation, growth of tumor xenograft in vivo, and arrested the cell cycle in the G1 phase. PMID: 27448304
    8. we suggest that TGIF plays an important role in low-dose arsenic-induced malignant transformation of HaCaT cells, which is regulated by c-Src/EGFR/AKT/FOXO3A pathway and redox signaling. PMID: 25537191
    9. Corneal fibroblasts demonstrate the expression of TGIF1 and TGIF2 transcription factors. These transcriptional repressors are critical, at least partially, in mediating the antifibrotic effect of vorinostat in the cornea. PMID: 26330748
    10. Our results suggested that elevated expression of TGIF was involved in lung carcinogenesis. PMID: 26091794
    11. our study demonstrated the oncogenic role of TGIF1 in NSCLC, and TGIF1 might be a therapeutic target for non-small cell lung cancer . PMID: 26104768
    12. c-Src/AKT is the upstream signaling that regulates TGIF-induced Nox4 activation and subsequent superoxide production. PMID: 25841779
    13. TGIF1 has a role as a negative regulator of MLL-rearranged acute myeloid leukemia PMID: 25349154
    14. TG-interacting factor transcriptionally induced by AKT/FOXO3A is a negative regulator that antagonizes arsenic trioxide-induced cancer cell apoptosis. PMID: 25791921
    15. TG-interacting factor 1 (Tgif1) is an important repressor of SOAT2 gene expression. PMID: 24478032
    16. TGIF1 is a relevant candidate gene with potential to contribute in the genesis of high myopia. PMID: 24215395
    17. Alternative splicing of TGIF1 is deregulated in OSCC, with overexpression of some splicing variants, especially TGIF1v8, which is associated with advanced stages of OSCC. PMID: 24119525
    18. Data demonstrate that homeobox gene TGIF-1 is a potential up-stream regulator of trophoblast differentiation and the altered TGIF-1 expression may contribute to aberrant villous trophoblast differentiation in FGR. PMID: 23761267
    19. 2 mutations in the holoprosencephaly (HPE)-related genes were detected (c.799 C>T, p.Q267X in TGIF gene and c.1279G>A, p.G427R in SHH gene) in 2 patients with pituitary stalk interruption syndrome (PSIS); data suggest HPE-related gene mutations are implicated in the etiolog of isolated pituitary defects PMID: 23476075
    20. TGIF1 plays a role in TNF-alpha- and radiation-induced inflammation and it could be a target in limiting this event in the vascular compartment PMID: 22995913
    21. biochemical analysis of how to overcome TALE DNA binding domain sensitivity to cytosine methylation PMID: 23019344
    22. TGIF can promote cellular migration/invasion activity of urothelial carcinoma cells. PMID: 22728270
    23. Exclusion of mutations in TGIF gene in patients with the syndrome of frontonasal dysgenesis, callosal agenesis, basal encephalocele, and eye anomalies PMID: 22496059
    24. TGIF contributes to the progression of urothelial carcinoma via the phosphatidylinositol 3-kinase-AKT pathway. PMID: 22771156
    25. Mutations in TGIF is associated with holoprosencephaly. PMID: 22310223
    26. There was a positive correlation between the severity of the brain malformation and facial features for SHH, SIX3, and TGIF, but no such correlation was found for ZIC2 mutations. PMID: 21940735
    27. Longer survival was associated with hypomethylation at specific CpG sites (e.g. GREB1, TGIF and TOB1) and hypermethylation in other genes (e.g. TMCO5, PTPRN and GUCY2C). PMID: 21577013
    28. The Smad corepressor transforming growth interacting factor was found to have flow-dependent nuclear localization PMID: 21490324
    29. TGIF1 transcript signals were frequently intense in normal tissue, and generally weak in oral squamous cell carcinoma (OSCC), and stronger transcript signals in well-differentiated areas of OSCC when compared with poorly differentiated ones. PMID: 21237437
    30. The mutation percentage (24%) is comparable with previous reports, but we detected significantly less mutations in SHH and significantly more in SIX3. For TGIF1 and ZIC2 mutation the rate was in conformity with earlier reports PMID: 20531442
    31. TGIF is a potential repressor of TGF-beta pathways in myometrial cells. PMID: 19524896
    32. Data show that activation of TNF-alpha signaling induced the association of TGIF with Itch/AIP4, resulting in increased accessibility of cFlip(L) for association and ubiquitination by Itch/AIP4. PMID: 20064471
    33. Data suggest that transforming growth-interacting factor (TGIF) has an important role myelopoiesis and may regulate the balance between proliferation and differentiation. PMID: 19699159
    34. molecular analysis of holoprosencephaly identified a novel heterozygous missense TGIF mutation PMID: 11810641
    35. The first nonsense mutation and also a novel missense mutation identified in tgif gene is associated with holoprosencephaly PMID: 12522553
    36. TGF-beta affects the gene expression regulation of this protein in normal and tumor cell lines. PMID: 12593671
    37. TGIF is a probable candidate gene for high myopia. PMID: 12601022
    38. Mutation analysis of the encoded TGIF gene for MYP2 autosomal dominant high myopia did not identify sequence alterations associated with the disease phenotype. PMID: 15223781
    39. Hypermethylation of cellular retinol-binding protein 1 is associated with gastric carcinogenesis PMID: 16134180
    40. The interaction of TGIF with cPML through c-Jun may negatively regulate TGF-beta signaling through controlling the localization of cPML and, consequently, the assembly of the cPML-SARA complex. PMID: 16916642
    41. Of the eleven sequence variations in TGIF, all but four can be demonstrated to be functionally abnormal, associated with holoprosencephaly. PMID: 16962354
    42. We found no statistical association between any of the 13 SNPs located on the TGIF gene and high myopia in Japanese subjects. PMID: 17048038
    43. results reveal an important mechanism for the degradation of TGIF through the ubiquitin-proteasome pathway, whose deregulation might contribute to the development of human holoprosencephaly PMID: 17158784
    44. This results point to the possible involvement of TGIF in the pathophysiology of psychotic disorders in the CVCR population. PMID: 17440433
    45. A Brazilian boy with lobar holoprosencephaly has a K44N mutation in the TGIF gene. PMID: 17825514
    46. Findings of this study suggest that the TGIF gene is unlikely to play a major role in either ocular biometric measures or refraction in a Caucasian population. PMID: 18172074
    47. TGIF is essential for EGF-mediated downregulation of tropoelastin expression PMID: 18441095
    48. A detailed description of the TGIF locus characterizing 12 TGIF splice isoforms. PMID: 18455519

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  • 相关疾病：
    Holoprosencephaly 4 (HPE4)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TALE/TGIF homeobox family
  • 数据库链接：

    HGNC: 11776

    OMIM: 142946

    KEGG: hsa:7050

    STRING: 9606.ENSP00000327959

    UniGene: Hs.373550