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  4. Recombinant Human Homeobox protein SIX1 (SIX1)

Recombinant Human Homeobox protein SIX1 (SIX1)

  • 货号：
    CSB-YP618986HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP618986HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP618986HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP618986HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP618986HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SIX1
  • Uniprot No.：
  • 别名：
    BOS3; DFNA23; Homeobox protein SIX1; OTTHUMP00000179042; Sine oculis homeobox homolog 1; SIX homeobox 1; SIX1; SIX1_HUMAN; TIP39
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-284
  • 氨基酸序列
    MSMLPSFGFT QEQVACVCEV LQQGGNLERL GRFLWSLPAC DHLHKNESVL KAKAVVAFHR GNFRELYKIL ESHQFSPHNH PKLQQLWLKA HYVEAEKLRG RPLGAVGKYR VRRKFPLPRT IWDGEETSYC FKEKSRGVLR EWYAHNPYPS PREKRELAEA TGLTTTQVSN WFKNRRQRDR AAEAKERENT ENNNSSSNKQ NQLSPLEGGK PLMSSSEEEF SPPQSPDQNS VLLLQGNMGH ARSSNYSLPG LTASQPSHGL QTHQHQLQDS LLGPLTSSLV DLGS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2. Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter and CIDEA enhancer. Regulates the expression of numerous genes, including MYC, CCND1 and EZR. Acts as activator of the IGFBP5 promoter, probably coactivated by EYA2. Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex. During myogenesis, seems to act together with EYA2 and DACH2. Regulates the expression of CCNA1. Promotes brown adipocyte differentiation.
  • 基因功能参考文献：
    1. SIX1 glycolytic function is directly repressed by microRNA-548a-3p, which is downregulated, inversely correlates with SIX1, and is a good predictor of prognosis in breast cancer patients PMID: 29455928
    2. Knockdown of SIX1 increased cell ROS levels and autophagy, promoted cell apoptosis, and enhanced TAX sensitivity of HepG2 cells. PMID: 29656300
    3. SIX1/EYA1 mutations might be partially responsible for conotruncal heart defects. PMID: 29043394
    4. We replicated the association of SNP rs10483727 in the SIX1/SIX6 locus with POAG in a Saudi cohort, suggesting its role in increasing susceptibility to Primary Open Angle Glaucoma . PMID: 29190129
    5. SIX1 is a potential target gene of miR-30a and down-regulation of SIX1 by siRNA inhibited proliferation and invasion of prostate cancer cells. PMID: 28573504
    6. Six1 is overexpressed in human primary pancreatic ductal adenocarcinomas and that its inhibition results in a decreased tumour progression in vitro and in vivo. PMID: 28388884
    7. in non-small cell lung cancer, SIX1-5 were associated with the greater possibility of the tumorigenesis PMID: 27821176
    8. SIX1 oncoprotein is aberrantly expressed in the endometrium following developmental exposure to estrogenic chemicals, correlates with uterine cancer, and is a biomarker in human endometrial cancers PMID: 27259717
    9. SIX homeobox 1 (SIX1) regulates cellular senescence by a p16INK4A (p16)-dependent mechanism. PMID: 26500063
    10. Studies strongly suggest that Six1 overexpression promotes CRC growth and metastasis and remodels tumor stroma by stimulating angiogenesis and recruiting TAM. MAPK activation may be a pivotal event in Six1-associated tumor progression. PMID: 28199476
    11. Restoration of SIX1 was sufficient to abolish proliferation, migration and invasion induced by miR-362 overexpression in cervical cancer cells. The newly identified miR-362/SIX1 pathway provides insight into cervical cancer progression, and may represent a novel therapeutic target. PMID: 27878258
    12. study replicated the association of POAG with two SNPs at the SIX1-SIX6 locus and demonstrated that SNPs, rs10483727 and rs33912345, are significantly associated with POAG, especially with NTG in patients aged above 40 years PMID: 27260188
    13. results suggest that SIX1 is a key proliferation regulator in mouse DFCs and human PDLCs, which provides novel insight into Six family gene function in mammals. PMID: 27241908
    14. miR-188 suppresses proliferation and invasion by targeting SIX1 in oral squamous cell carcinoma cells. PMID: 26490981
    15. Upregulation of Six1 could downregulate miR-204-5p expression. PMID: 26408179
    16. Mutations of SIX1 gene underlie Wilms tumor recurrences. PMID: 26802027
    17. Six1 signaling has a role in paclitaxel-dependent apoptosis in MCF-7 cell line PMID: 26773176
    18. Six1 mutations/gene deletion are unlikely to be a major cause of nonsyndromic CAKUT. PMID: 24899122
    19. Data show that the protein domain interfaces may represent therapeutic targets in homeo domain protein SIX1-positive Hodgkin lymphoma (HL) subsets. PMID: 26473286
    20. Data show that both Ezrin and SIX1 proteins are highly expressed in alpha fetoprotein-negative hepatocellular carcinoma (HCC) and significantly related with the TNM stage. PMID: 26927385
    21. These data suggest differential SIX-factor regulation might have contributed to species differences in nephron progenitor programs such as the duration of nephrogenesis and the final nephron count PMID: 26884396
    22. Single nucleotide polymorphism in SIX1 gene is associated with primary open angle glaucoma. PMID: 25798827
    23. High expression of SIX1 is an independent prognostic marker in colorectal cancer. PMID: 25951369
    24. Three causative genes for BOR syndrome have been reported thus far: EYA1, SIX1, and SIX5, but the causative genes for approximately half of all BOR patients remain unknown.[review] PMID: 24730701
    25. SIX1 and EYA are often co-overexpressed in tumors, and the SIX1-EYA2 interaction has been shown to be critical for metastasis in a breast cancer model. PMID: 25555392
    26. Six1 plays an important role in the TIC population in luminal breast cancers and induces a TIC phenotype by enhancing both TGF-beta and ERK signaling. PMID: 22765220
    27. SIX1 plays an important role in the progression ofhepatocellular carcinoma PMID: 25031720
    28. in tumors with DGCR8 E518K and DROSHA exon 29 (miRNAPG-HS) mutations ... greater prevalence of tumors with blastemal predominant histology in patients with miRNAPG-HS and/or SIX1/2 Q177R mutations PMID: 25670082
    29. Recurrent mutations included a hotspot mutation (Q177R) in the homeo-domain of SIX1 and SIX2 in tumors with high proliferative potential (18.1% of blastemal cases); mutations in the DROSHA/DGCR8 microprocessor genes PMID: 25670083
    30. Six1 overexpression in HPV16-immortalized keratinocytes increased cell proliferation and promoted cell migration and invasion by inducing epithelial-mesenchymal transition. PMID: 25463612
    31. HDAC5 promoted the Six1 expression. PMID: 24706304
    32. tumor cells that expressed high levels of SIX1 could promote lymphangiogenesis and counteract the negative effects of TGFbeta on lymphangiogenesis by increasing the expression of VEGF-C PMID: 25142796
    33. Results suggest that the increase of SIX1 expression could promote tumorigenesis, progression and invasive growth of cervical cancer by promoting DNA replication. PMID: 24970368
    34. In breast phyllodes tumors, Six1 and Pax3 expression is correlated with tumour grade, unfavourable clinicopathological parameters and poorer clinical outcome, suggesting that both proteins may play a role in malignant progression. PMID: 24438019
    35. SIX1 was frequently upregulated in gastric adenocarcinoma specimens and its overexpression could potentially be used as an independent predictor of prognosis and survival time in patients with gastric cancer PMID: 24866365
    36. Suggest that lentivirus-mediated Six1 inhibition may represent a novel therapeutic approach for treatment of colorectal cancer. PMID: 24551283
    37. Six1 promotes epithelial-mesenchymal transition and malignant conversion in human papillomavirus type 16-immortalized human keratinocytes. PMID: 24574515
    38. In colorectal cancer cells, SIX1 is a target gene for regulation of cell migration and tumor invasion. PMID: 24593661
    39. data suggested that Six1 might be involved in the promotion of growth, proliferation, and migration of osteosarcoma cell lines PMID: 24114014
    40. These results suggest that SIX1/alpha5beta1 might be considered as valuable marker for metastatic potential of cervical cancer cells, or a therapeutic target in cervical cancer treatment. PMID: 24613848
    41. SIX1 overexpression is associated with pancreatic ductal adenocarcinoma. PMID: 24263054
    42. these data suggest that Six1 may function as an important modifier of the paclitaxel response in breast cancer cells, and serve as a potential target for overcoming paclitaxel resistance in breast cancer. PMID: 24184484
    43. Six1 promotes proliferation of pancreatic cancer cells via upregulation of cyclin D1 expression. PMID: 23527134
    44. Given that SIX1 and EYA are overexpressed in many tumor types, our data indicate that targeting the SIX1-EYA complex may be a potent approach to inhibit tumor progression in multiple cancer types PMID: 23435380
    45. It is required downstream of Six1 to induce these phenotypes. PMID: 22286770
    46. SIX1 overexpression contributes to epithelial-mesenchymal transition partly through repression of miR-200-family expression and activation of ZEB1 in colorectal cancer. PMID: 22286765
    47. The identification of SIX1 and CDKN2B variant was found to be associated more strongly with advanced open-angle glaucoma. PMID: 22840486
    48. An exception is the predominant expression of SIX1 in blastemal cells, hereby identifying this protein as a candidate marker for blastema. PMID: 22180226
    49. In East Asian populations, a SIX1 mutation has been reported in a Japanese family with branchio-oto (BO) syndrome. PMID: 22447252
    50. A critical role for SIX1 in lymphatic dissemination of breast cancer cells, providing a direct mechanistic explanation for how VEGF-C expression is upregulated in breast cancer. PMID: 22466647

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  • 相关疾病：
    Deafness, autosomal dominant, 23 (DFNA23); Branchiootic syndrome 3 (BOS3)
  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    SIX/Sine oculis homeobox family
  • 组织特异性：
    Specifically expressed in skeletal muscle.
  • 数据库链接：

    HGNC: 10887

    OMIM: 601205

    KEGG: hsa:6495

    STRING: 9606.ENSP00000247182

    UniGene: Hs.633506