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  4. Recombinant Human Histone-lysine N-methyltransferase SETMAR (SETMAR)

Recombinant Human Histone-lysine N-methyltransferase SETMAR (SETMAR)

  • 货号：
    CSB-YP703613HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP703613HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP703613HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP703613HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP703613HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SETMAR
  • Uniprot No.：
  • 别名：
    Histone lysine N methyltransferase; Histone lysine N methyltransferase SETMAR; Hsmar 1; Hsmar1; Mariner transposase Hsmar1; Metnase; SET domain and mariner transposase fusion; SET domain and mariner transposase fusion gene; SET domain and mariner transposase fusion gene containing protein; SET domain and mariner transposase fusion gene-containing protein; Setmar; SETMR_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-671
  • 氨基酸序列
    MAEFKEKPEA PTEQLDVACG QENLPVGAWP PGAAPAPFQY TPDHVVGPGA DIDPTQITFP GCICVKTPCL PGTCSCLRHG ENYDDNSCLR DIGSGGKYAE PVFECNVLCR CSDHCRNRVV QKGLQFHFQV FKTHKKGWGL RTLEFIPKGR FVCEYAGEVL GFSEVQRRIH LQTKSDSNYI IAIREHVYNG QVMETFVDPT YIGNIGRFLN HSCEPNLLMI PVRIDSMVPK LALFAAKDIV PEEELSYDYS GRYLNLTVSE DKERLDHGKL RKPCYCGAKS CTAFLPFDSS LYCPVEKSNI SCGNEKEPSM CGSAPSVFPS CKRLTLETMK MMLDKKQIRA IFLFEFKMGR KAAETTRNIN NAFGPGTANE RTVQWWFKKF CKGDESLEDE ERSGRPSEVD NDQLRAIIEA DPLTTTREVA EELNVNHSTV VRHLKQIGKV KKLDKWVPHE LTENQKNRRF EVSSSLILRN HNEPFLDRIV TCDEKWILYD NRRRSAQWLD QEEAPKHFPK PILHPKKVMV TIWWSAAGLI HYSFLNPGET ITSEKYAQEI DEMNQKLQRL QLALVNRKGP ILLHDNARPH VAQPTLQKLN ELGYEVLPHP PYSPDLLPTN YHVFKHLNNF LQGKRFHNQQ DAENAFQEFV ESQSTDFYAT GINQLISRWQ KCVDCNGSYF D
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

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靶点详情

  • 功能：
    Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Has also kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity. In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining. Also regulates replication fork processing, promoting replication fork restart and regulating DNA decatenation through stimulation of the topoisomerase activity of TOP2A.
  • 基因功能参考文献：
    1. Various SETMAR proteins can be synthesized in human glioblastoma that may each have specific biophysical and/or biochemical properties and characteristics. PMID: 28038463
    2. These results suggest that Metnase enhances Exo1-mediated exonuclease activity on the lagging strand DNA by facilitating Exo1 loading onto a single strand gap at the stalled replication fork. PMID: 27974460
    3. The SET domain is needed for the 5' end of ss-overhang cleavage with fork and non-fork DNA without affecting the Metnase-DNA interaction. This domain has a positive role in restart of replication fork and the 5' end of ss-overhang cleavage. PMID: 26437079
    4. methylation of snRNP70 by SETMAR regulates constitutive and/or alternative splicing PMID: 25795785
    5. Metnase may possess an important role in DNA repair, topoisomerase II function, and the maintenance of stemness during colon cancer development. PMID: 25333365
    6. 293 T transfected with Metnase revealed a large number of rescued plasmids. PMID: 24655462
    7. found known and novel SETMAR splice variants to be significantly increased in acute myeloid leukemia PMID: 24607956
    8. a single mutation DDN(610) --> DDD(610), which restores the ancestral catalytic site, results in loss of function in Metnase PMID: 24573677
    9. phosphorylation of Metnase S495 differentiates between these two functions, enhancing DSB repair and repressing replication fork restart. PMID: 22231448
    10. a role for Metnase's endonuclease activity in promoting the joining of noncompatible ends PMID: 21491884
    11. Data show that DBN1, SETMAR and HIG2 are direct transcriptional targets of the SOX11 protein. PMID: 21124928
    12. DNA repair protein Metnase (also SETMAR), which has a SET histone methylase domain, localized to an induced DSB and directly mediated the formation of H3K36me2 near the induced DSB PMID: 21187428
    13. results establish Metnase as a key factor that promotes restart of stalled replication forks, and implicate Metnase in the repair of collapsed forks. PMID: 20457750
    14. hPso4, once it forms a complex with Metnase, negatively regulates Metnase's TIR binding activity PMID: 20416268
    15. Metnase is a nonhomologous end-joining repair protein that regulates genomic integration of exogenous DNA and establishes a relationship among histone modification, DNA repair, and integration. PMID: 16332963
    16. These data suggest that vectors based on the Himar1 transposable element, in conjunction with the hyperactive mutant transposase C9, may be suitable vectors for gene therapy applications. PMID: 16989604
    17. SETMAR is unlikely to catalyze transposition in the human genome, although the nicking activity may have a role in the DNA repair phenotype. PMID: 17130240
    18. The activities of the SETMAR protein on transposon ends are described. PMID: 17403897
    19. Results suggest that Metnase's DNA cleavage activity, unlike those of other eukaryotic transposases, is not coupled to its sequence-specific DNA binding. PMID: 17877369
    20. hPso4 is necessary to bring Metnase to the DSB sites for its function(s) in DNA repair PMID: 18263876
    21. Metnase physically interacts and co-localizes with Topoisomerase IIalpha, the key chromosome decatenating enzyme. PMID: 18790802
    22. myeloid leukemia cells fail to arrest at the mitotic decatenation checkpoint, and their progression through this checkpoint is regulated by the DNA repair component Metnase (also termed SETMAR) PMID: 19458360

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Class V-like SAM-binding methyltransferase superfamily; Mariner transposase family
  • 组织特异性：
    Widely expressed, with highest expression in placenta and ovary and lowest expression in skeletal muscle.
  • 数据库链接：

    HGNC: 10762

    OMIM: 609834

    KEGG: hsa:6419

    STRING: 9606.ENSP00000373354

    UniGene: Hs.475300