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  4. Recombinant Human Guanine nucleotide-binding protein subunit alpha-13 (GNA13)

Recombinant Human Guanine nucleotide-binding protein subunit alpha-13 (GNA13)

  • 货号：
    CSB-EP618885HU
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    G alpha 13; G alpha-13; G-protein subunit alpha-13; GNA13; GNA13_HUMAN; Guanine nucleotide binding protein (G protein) alpha 13; Guanine nucleotide binding protein alpha 13 subunit; Guanine nucleotide-binding protein subunit alpha-13
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    48.0 kDa
  • 表达区域：
    1-377aa
  • 氨基酸序列
    MADFLPSRSVLSVCFPGCLLTSGEAEQQRKSKEIDKCLSREKTYVKRLVKILLLGAGESGKSTFLKQMRIIHGQDFDQRAREEFRPTIYSNVIKGMRVLVDAREKLHIPWGDNSNQQHGDKMMSFDTRAPMAAQGMVETRVFLQYLPAIRALWADSGIQNAYDRRREFQLGESVKYFLDNLDKLGEPDYIPSQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVSSSEFDQVLMEDRLTNRLTESLNIFETIVNNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPHCLRDVQKFLVECFRNKRRDQQQKPLYHHFTTAINTENIRLVFRDVKDTILHDNLKQLMLQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation.
  • 基因功能参考文献：
    1. High expression of GNA13 is associated with hepatocellular carcinoma. PMID: 27883022
    2. The ability of enhanced GNA13 signaling to suppress KLK gene expression appears at least in part due to the ability of enhanced GNA13 signaling to negatively impact Rho/ROCK-signaling. PMID: 27424208
    3. the selective expression of FZD10 in brain vascular endothelial cells points at a potential role of FZD10-Galpha13 signalling in central nervous system angiogenesis. PMID: 28126591
    4. The protein profile indicates attenuation of "GNA13-ERK signaling" in schizophrenia brain. In particular, EIF4G2 and CYFIP1, which are located downstream of the GNA13-ERK network, were decreased, suggesting that the attenuation of this signal network may cause impairment of axon formation and synapse plasticity in the brain of schizophrenia patients. PMID: 28214564
    5. Findings indicate a tumor suppressive role for G protein subunit alpha 13 (Galpha13) and rhoA GTP-binding protein (RhoA) in Burkitt's lymphoma and diffuse large B-cell lymphoma (DLBCL). PMID: 26616858
    6. Data (including data from studies using transgenic mice) suggest that R7BP-RGS7 heterotrimers interact with Galpha13 to augment signaling pathways in neurons that regulate neurite morphogenesis. (R7BP = RGS7 family binding protein; RGS7 = regulator of G-protein signaling 7 protein; Galpha13 = GTP-binding protein alpha subunit 13) PMID: 28432124
    7. Upregulation of GNA13 expression increased the proliferation and tumorigenicity of gastric cancer. PMID: 26735177
    8. Knockdown DDR1 reversed the effects of Galpha13 knockdown on cell-cell adhesion and proteolytic invasion in three-dimensional collagen. PMID: 26589794
    9. RGS22 acts as a tumor suppressor, repressing human pancreatic adenocarcinoma cell migration by coupling to GNA12/13. PMID: 26323264
    10. Galpha13 Switch Region 2 Binds to the Talin Head Domain and Activates alphaIIbbeta3 Integrin in Human Platelets PMID: 26292217
    11. GNA13 expression in breast cancer cells is regulated by post-transcriptional mechanisms involving miR-31. PMID: 25889182
    12. Data show that N-linked glycosylation of protease-activated receptor-1 (PAR1) at extracellular loop 2 (ECL2) controls G12/13 versus Gq G-proteins coupling specificity in response to thrombin stimulation. PMID: 26100877
    13. Our findings highlight the important role of miR-29c in regulating CRC EMT via GSK-3b/b-catenin signaling by targeting GNA13 and PTP4A and provide new insights into the metastatic basis of colorectal cancer PMID: 25193986
    14. These results place Blk upstream of the p190RhoGAP-RhoA pathway in Galpha13-activated cells, overall representing an opposing signaling module during CXCL12-triggered invasion. PMID: 25025568
    15. findings identify a Galpha13-dependent pathway that exerts dual actions in suppressing growth and blocking dissemination of germinal centre B cells that is frequently disrupted in germinal centre B-cell-derived lymphoma PMID: 25274307
    16. Findings indicate lysophosphatidic acid (LPA)-lysophosphatidic acid receptor-Galpha13 signaling node as a therapeutic target for pancreatic cancer treatment and control. PMID: 23508014
    17. Mutation in the Galpha13 gene is associated with diffuse large B-cell lymphoma. PMID: 23699601
    18. Itk enhances Galpha13 mediated activation of serum response factor (SRF) transcriptional activity dependent on its ability to interact with Galpha13, but its kinase activity is not required to enhance SRF activity. PMID: 23454662
    19. GNA13 is an important mediator of prostate cancer cell invasion, and miR-182 and miR-200 family members regulate its expression post-transcriptionally PMID: 23329838
    20. Activation of p115-RhoGEF requires direct association of Galpha13 and the Dbl homology domain. PMID: 22661716
    21. signaling-selective inhibition of the CXCR4-Galpha(13)-Rho axis prevents the metastatic spread of basal-like breast cancer cells. PMID: 21934106
    22. alpha subunit of the G protein G13 regulates activity of one or more Gli transcription factors independently of smoothened PMID: 21757753
    23. Negative regulation of Gq-mediated pathways in platelets by G(12/13) pathways through Fyn kinase PMID: 21592972
    24. Galpha(12/13) regulate AP-1-dependent CYR61 induction in vascular smooth muscle, promoting migration, and they are upregulated with CYR61 in arteriosclerotic lesions. PMID: 21212405
    25. A new role is described for Galpha13 as a mediator of chemokine receptor CXCR4 endosomal trafficking in human T cells. PMID: 21148034
    26. Galpha(12) binds to p120(ctn) and modulates its phosphorylation status through a Rho-independent mechanism. Galpha(12) emerges as an important regulator of p120(ctn) function PMID: 20974127
    27. Galpha12/13 upregulate matrix metalloproteinase-2 via p53 promoting human breast cell invasion. PMID: 20044778
    28. G(alpha)(12) and G(alpha)13) exert a complex pattern of nonredundant effects in small cell lung cancer cells PMID: 20160064
    29. This review gives an overview of Galpha12/13 signaling of G protein-coupled receptors with a focus on RhoGTPase nucleotide exchange factor (RhoGEF) proteins as the immediate mediators of G12/13 activation. PMID: 19226283
    30. The studies highlighted in this review provide compelling evidence that Galpha12 and Galpha13 play pivotal roles in many aspects of cancer invasion and metastasis. PMID: 19422395
    31. study demonstrates not only a function of integrin alphaIIbbeta3 as a noncanonical Galpha13-coupled receptor but also a new concept of Galpha13-dependent dynamic regulation of RhoA PMID: 20075254
    32. Galpha12 and Galpha13 negatively regulate the adhesive functions of cadherin PMID: 11976333
    33. Galpha13 is not associated with lipid rafts PMID: 12117999
    34. Galpha13 can induce ppET-1 gene expression through a JNK-mediated pathway. PMID: 12135322
    35. co-stimulation of G(12/13) and G(i) pathways is sufficient to activate GPIIb/IIIa in human platelets in a mechanism that involves intracellular calcium PMID: 12297512
    36. These results suggest that protein kinase A blocks Rho activation by phosphorylation of Galpha(13) Thr(203). PMID: 12399457
    37. Selective activation of Galpha(12) and Galpha(13) by thrombin and LPA, respectively, is determined by the N-terminal short sequences of alpha subunits. PMID: 12594220
    38. Several features of a typical alpha/RGS interaction are preserved in the alpha(13)/p115RhoGEF interaction. PMID: 15735747
    39. Galpha13-induced VASP phosphorylation that involves activation of RhoA and MEKK1, phosphorylation and degradation of IkappaB, release of PKA catalytic subunit from the complex with IkappaB and NF-kappaB, and subsequent phosphorylation of VASP PMID: 16046415
    40. analysis of the molecular mechanism of how the human TXA2 receptor interacts with G alpha 13 to activate intracellular signaling PMID: 16212421
    41. Estrogen receptor alpha interacts with Galpha13 to drive actin remodeling and endothelial cell migration. PMID: 16601072
    42. Results identify the G12 family proteins Galpha12 and 13 as important regulators of prostate cancer invasion and suggest that these proteins may be targeted to limit invasion- and metastasis-induced prostate cancer patient mortality. PMID: 16787920
    43. Galpha13-Rho signaling axis is required for SDF-1-induced migration through CXCR4 PMID: 17056591
    44. Distinct regions of Galpha13 participate in its regulatory interactions with RGS homology domain-containing RhoGEFs. PMID: 17449226
    45. analysis of a novel cross-talk exerted from the LPA/Galpha(13)/p115RhoGEF/RhoA pathway to the beta(2)-adrenergic receptor/Galpha(s)/adenylyl cyclase pathway PMID: 17493936
    46. G alpha(12/13) regulate basal p53 levels via mdm4, which constitutes a cell signaling pathway distinct from p53 phosphorylations elicited by genotoxic stress. PMID: 17510313
    47. role in regulating ASK1 expression levels PMID: 17595347
    48. provides first examination of Galpha12 and Galpha13 in the human heart, demonstrating selective activation of human atrial Galpha12 and Galpha13 by endothelin and angiotensin receptors, respectively PMID: 17878759
    49. A pronounced and rapid translocation of p115-RhoGEF from the cytosol to the plasma membrane was observed upon activation of several G(12/13)-coupled receptors in a cell type-independent fashion. PMID: 18320579
    50. AC7 is a specific downstream effector of the G(12/13) pathway PMID: 18541530

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor. Melanosome. Cytoplasm. Nucleus.
  • 蛋白家族：
    G-alpha family, G(12) subfamily
  • 组织特异性：
    Expressed in testis, including in Leydig cells and in the seminiferous epithelium, in differentiating cells from the spermatogonia to mature spermatozoa stages and round spermatids (at protein level). Expressed in 99.2% of spermatozoa from healthy individ
  • 数据库链接：

    HGNC: 4381

    OMIM: 604406

    KEGG: hsa:10672

    STRING: 9606.ENSP00000400717

    UniGene: Hs.515018