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  4. Recombinant Human Growth factor receptor-bound protein 7 (GRB7)

Recombinant Human Growth factor receptor-bound protein 7 (GRB7)

  • 货号：
    CSB-YP009890HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009890HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009890HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009890HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009890HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GRB7
  • Uniprot No.：
  • 别名：
    B47; Epidermal growth factor receptor GRB 7; Epidermal growth factor receptor GRB-7; GRB7 adapter protein; GRB7; GRB7_HUMAN; Growth factor receptor bound protein 7; Growth factor receptor-bound protein 7; OTTHUMP00000164349; OTTHUMP00000164350; OTTHUMP00000164351; OTTHUMP00000164352
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-532
  • 氨基酸序列
    MELDLSPPHL SSSPEDLCPA PGTPPGTPRP PDTPLPEEVK RSQPLLIPTT GRKLREEERR ATSLPSIPNP FPELCSPPSQ SPILGGPSSA RGLLPRDASR PHVVKVYSED GACRSVEVAA GATARHVCEM LVQRAHALSD ETWGLVECHP HLALERGLED HESVVEVQAA WPVGGDSRFV FRKNFAKYEL FKSSPHSLFP EKMVSSCLDA HTGISHEDLI QNFLNAGSFP EIQGFLQLRG SGRKLWKRFF CFLRRSGLYY STKGTSKDPR HLQYVADVNE SNVYVVTQGR KLYGMPTDFG FCVKPNKLRN GHKGLRIFCS EDEQSRTCWL AAFRLFKYGV QLYKNYQQAQ SRHLHPSCLG SPPLRSASDN TLVAMDFSGH AGRVIENPRE ALSVALEEAQ AWRKKTNHRL SLPMPASGTS LSAAIHRTQL WFHGRISREE SQRLIGQQGL VDGLFLVRES QRNPQGFVLS LCHLQKVKHY LILPSEEEGR LYFSMDDGQT RFTDLLQLVE FHQLNRGILP CLLRHCCTRV AL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress.
  • 基因功能参考文献：
    1. MiR-193a-3p not only modulated GRB7 but also ERBB4. PMID: 29290818
    2. Our results indicated that Grb7 over-expression may facilitate invasion and inhibit apoptosis in cervical cancer PMID: 28780081
    3. data shows that GRB7 expression in invasive breast cancer correlates with markers of a more aggressive phenotype, including HER2 overexpression, a greater degree of HER2 amplification, ER negativity, and p53 positivity PMID: 26945445
    4. The crystal structure of a stapled bicyclic peptide inhibitor G7-B1 in complex with the Grb7-SH2 domain. PMID: 27257138
    5. Grb7 protein Ras-associating domain oligomerization has been reported. PMID: 28295715
    6. Study provided a direct link between the signal adapter protein Grb7, anti-apoptotic protein Hax1 isoform 1, and Caspase3-mediated apoptosis pathways, and suggests that besides modulating cell migration and signal transduction, Grb7 may also participate in Hax1-related apoptosis pathways mediated by Caspase3. PMID: 26869103
    7. Suggest close relationship between Grb7 gene amplification and GRB7 protein overexpression in human ovarian cancer. Immunohistochemistry might have limited diagnostic value in these tumors compared to fluorescence in situ hybridization. PMID: 26617853
    8. Grb7 was found to be significantly related to the biological classification of breast cancer PMID: 25182704
    9. apo Grb7 SH2 domain crystallized in the trigonal space group P63, whereas the G7-B1-Grb7 SH2 domain complex crystallized in the monoclinic space group P21 PMID: 24637751
    10. The data reveal that Grb7 plays an important role in breast cancer progression, beyond the context of HER2+ve cell types PMID: 24464577
    11. Data suggest that calmodulin controls Grb7-mediated cell migration. PMID: 23743201
    12. Data propose the phosphorylation state of Grb7-SH2 domain tyrosine residues could control Grb7 dimerization, and dimerization may be an important regulatory step in Grb7 binding to RTKs such as erbB2. PMID: 22811067
    13. GRB7 is a context-dependent oncogene, which modulates the ERBB2 signaling pathway through enhanced phosphorylation of ERBB2 and Akt. PMID: 22584052
    14. propose that CaM inhibits the translocation of Grb7 to the nucleus after binding to its CaM-BD and therefore occluding its overlapping NLS PMID: 22673522
    15. A series of Grb7 SH2 domain-binding nonphosphorylated peptides in the yeast two-hybrid system, were identified. PMID: 22253820
    16. GRB7 protein over-expression is an independent adverse prognostic factor in human breast cancer PMID: 20635137
    17. Full-length Grb7 and Hax-1 interact in mammalian cells and Grb7 is tyrosine phosphorylated. PMID: 20665473
    18. X-ray diffraction data were collected from crystals to 2.4A resolution for G7-18NATE in complex with its Grb7 SH2 domain. PMID: 21139214
    19. proposed helix-swapping of the SH2 domain of Grb7, a regulatory protein implicated in cancer progression and inflammation PMID: 20370637
    20. Data show that Grb7 removal by RNA-interference reduces breast cancer cell viability. PMID: 20126311
    21. Findings illustrate an underlying mechanism by which Grb7 promotes tumorigenesis through the formation of a novel EGFR-Grb7-Ras signaling complex, thereby highlighting the potential strategy of targeting Grb7 as an anti-breast cancer therapy. PMID: 20622016
    22. Studies implicate that the overexpressed GRB7 and GRB7v are associated with high-grade tumors and exert distinct tumorigenic functions through regulating different signaling pathways in ovarian cancer cells. PMID: 20388850
    23. High Grb7 is associated with breast cancer. PMID: 19717535
    24. Identification of novel non-phosphorylated ligands, which bind selectively to the SH2 domain of Grb7 PMID: 11809769
    25. the PH domain mediated Grb7 binding to phospholipids PMID: 12021278
    26. NMR assignments of backbone 1H, 13C, and 15N resonances of Grb7-SH2 domain in complex with a phosphorylated peptide ligand PMID: 12061724
    27. review of GRB7 binding sites and molecular interactions, and role in progression and invasive potential in cancer, making it a target for therapeutics PMID: 14585167
    28. Incresaed Grb7 expression is associated with disease progression in chronic lymphocytic leukemia PMID: 15470489
    29. Human Grb7 specifically binds CaM in a calcium2+-dependent manner; possible alterations of the Grb7/CaM system in tumor cells could be relevant to high motility seen in many cancer cells PMID: 15806159
    30. Grb7-SH2 domain dimerisation depends on a single point mutation PMID: 15841400
    31. Mutation and elevated expression of GRB7 is associated with the development of testicular germ cell tumors PMID: 16354586
    32. Grb7 expression was statistically significantly different between specimens from patients with or without lymph node metastasis. PMID: 16595785
    33. The Grb7 peptide has potential to be developed as a therapeutic agent alone, or in combination with traditional chemotherapy. PMID: 17426702
    34. Grb7 plays an important role in hepatocellular carcinoma progression and is strongly associated with expression of focal adhesion kinase. PMID: 17634422
    35. The solution structure and the backbone relaxation behavior of the Grb7-SH2/erbB2 peptide complex. PMID: 17705331
    36. GRB-7 over-expression plays pivotal roles in activating signal transduction and promoting tumor growth in breast cancer cells with chromosome 17q11-21 amplification PMID: 17916906
    37. Formation of focal adhesion kinase *Grb7 complexes and Grb7 phosphorylation by FAK in an integrin-dependent manner were essential for cell migration, proliferation and anchorage-independent growth in A431 epidermal carcinoma cells. PMID: 19473962
    38. Results illuminate the membrane-recruitment mechanisms of Grb7, Grb10 and Grb14. PMID: 19648926
    39. Oncogenomic recombination hotspot around the PPP1R1B-STARD3-TCAP-PNMT-PERLD1-ERBB2-C17orf37-GRB7 amplicon at human chromosome 17q12 is closely linked to evolutionary recombination hotspot around the GSDML-GSDM locus. PMID: 15010812

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  • 亚细胞定位：
    Cytoplasm. Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic granule. Cell projection.
  • 蛋白家族：
    GRB7/10/14 family
  • 数据库链接：

    HGNC: 4567

    OMIM: 601522

    KEGG: hsa:2886

    STRING: 9606.ENSP00000403459

    UniGene: Hs.86859