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  4. Recombinant Human Growth arrest and DNA damage-inducible proteins-interacting protein 1 (GADD45GIP1)

Recombinant Human Growth arrest and DNA damage-inducible proteins-interacting protein 1 (GADD45GIP1)

  • 货号：
    CSB-YP855033HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP855033HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP855033HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP855033HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP855033HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GADD45GIP1
  • Uniprot No.：
  • 别名：
    39S ribosomal protein L59 mitochondrial; CKBBP2; CKbetaBP2; CKII beta associating protein; CKII beta binding protein 2; CKII beta-associating protein; CR6 interacting factor 1; CR6-interacting factor 1; CRIF1; G45IP_HUMAN; GADD45G interacting protein 1; Gadd45gip1; Growth arrest and DNA damage inducible GADD45G interacting protein; growth arrest and DNA damage inducible, gamma interacting protein 1; Growth arrest and DNA damage-inducible proteins-interacting protein 1; MGC4667; MGC4758; MRP L59; p53 responsive gene 6; p53 responsive gene 6 protein; p53-responsive gene 6 protein; Papillomavirus L2 interacting nuclear protein 1; Papillomavirus L2-interacting nuclear protein 1; PLINP 1; PLINP; PLINP-1; PLINP1; PRG6
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-222
  • 氨基酸序列
    MAASVRQARS LLGVAATLAP GSRGYRARPP PRRRPGPRWP DPEDLLTPRW QLGPRYAAKQ FARYGAASGV VPGSLWPSPE QLRELEAEER EWYPSLATMQ ESLRVKQLAE EQKRREREQH IAECMAKMPQ MIVNWQQQQR ENWEKAQADK ERRARLQAEA QELLGYQVDP RSARFQELLQ DLEKKERKRL KEEKQKRKKE ARAAALAAAV AQDPAASGAP SS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity. May play a role in mitochondrial protein synthesis.
  • 基因功能参考文献：
    1. NRF2 Ser40 phosphorylation was inhibited in Crif1-deficient bone marrow multipotent mesenchymal stromal cells even in the presence of three kinds of PKC agonists, suggesting that CRIF1 might co-activate PKC-delta to phosphorylate NRF2 Ser40. PMID: 28819452
    2. strongly suggest that CRIF1 deficiency promotes endothelial cell inflammation by increasing VCAM-1 expression, elevating inflammatory cytokines levels, and activating the transcription factor NF-kappaB, all of which were inhibited by SIRT1 overexpression PMID: 29474366
    3. Results demonstrate that knockdown of CRIF1 in HUVECs induces mitochondrial dysfunction and reduces SIRT1 expression along with decreased eNOS phosphorylation and increased eNOS acetylation, thereby promoting endothelial dysfunction. These findings indicate that CRIF1 plays an important role in maintaining mitochondrial and endothelial function through its effects on the SIRT1-eNOS pathway. PMID: 28117598
    4. SNF5 is indispensable for CRIF1-enhanced p53 activity and its function in the suppression of cell cycle arrest in human cancer cells. PMID: 28235567
    5. Study shows that Lck interacts with CRIF1 in the mitochondria and negatively regulates CRIF1-mediated translation of mitochondrion-encoded proteins. PMID: 26210498
    6. our results support a novel function of nuclear Lck in promoting human leukemic T cell survival through interaction with a tumor suppressor, CRIF1 PMID: 25997448
    7. Crif1 is an indispensable regulator of PKAalpha cat that modulates the PKA/CREB signaling pathway to promote adipogenic differentiation of bone marrow mesenchymal stem cells after irradiation PMID: 25847389
    8. The results identify the ROS-Sp1-Crif1 pathway to be a new mechanism underlying Abeta-induced mitochondrial dysfunction and suggest that ROS-mediated downregulation of Crif1 is a crucial event in AD pathology. PMID: 25361083
    9. CRIF1 knockdown partially induces endothelial activation via increased ROS production and phosphorylation of p66shc PMID: 24906005
    10. CRIF1 may play a regulatory role in the BM microenvironment-induced leukemia cell cycle arrest possibly through interacting with CDK2 and acting as a cyclin-dependent kinase inhibitor. PMID: 24520316
    11. CKbetaBP2/CRIF1 is expressed with STAT3 in prostate cancer where STAT3 may help to offset the AR repressor effect of CKbetaBP2/CRIF1. PMID: 24103312
    12. results indicated cell cycle arrest of Jurkat cells in the G0/G1 phase to be induced by primary cultured leukemic BMSCs associated with increased expression of CRIF1 by leukemic cells PMID: 21911160
    13. CRIF1, unlike KEAP1 (which only interacts with N-terminal region of NRF2), physically interacts with both N- and C-terminal regions of NRF2 and promotes NRF2 ubiquitination and subsequent proteasome-mediated NRF2 protein degradation PMID: 20427290
    14. results suggest that CRIF1 is a novel nuclear protein that interacts with Gadd45 and may play a role in negative regulation of cell cycle progression and cell growth PMID: 12716909
    15. Phosphorylation of serine-221 in CKBBP2/CRIF1 promotes proliferation of green monkey COS7 cells. PMID: 17069992
    16. NAC-1 contributes to tumor growth and survival by at least inhibiting Gadd45GIP1 expression, which has a tumor suppressor effect in cancer cells. PMID: 17804717
    17. Results suggest that CRIF1 acts as an AR corepressor and may play an important role in the regulation of AR-positive growth of prostate cancer. PMID: 17885209

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  • 亚细胞定位：
    Mitochondrion. Nucleus.
  • 蛋白家族：
    Mitochondrion-specific ribosomal protein mL64 family
  • 组织特异性：
    Widely expressed. Highly expressed in the thyroid gland, heart, lymph nodes, trachea and adrenal tissues. Expressed at lower level in liver skeletal muscle, kidney, pancreas, testis, ovary and stomach. Barely detectable in adrenal adenoma and papillary th
  • 数据库链接：

    HGNC: 29996

    OMIM: 605162

    KEGG: hsa:90480

    STRING: 9606.ENSP00000323065

    UniGene: Hs.515164