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  4. Recombinant Human Glycine N-methyltransferase (GNMT)

Recombinant Human Glycine N-methyltransferase (GNMT)

  • 货号：
    CSB-YP009628HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009628HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009628HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009628HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009628HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GNMT
  • Uniprot No.：
  • 别名：
    EC 2.1.1.20; Epididymis secretory sperm binding protein Li 182mP; Glycine N methyltransferase; Glycine N-methyltransferase; Gnmt; GNMT_HUMAN; HEL S 182mP; OTTHUMP00000016412
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-295
  • 氨基酸序列
    VDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.
  • 基因功能参考文献：
    1. Elevated GNMT protein expression is associated with pathogenesis of hepatocellular carcinoma. PMID: 28205209
    2. In men of European descent, the GNMT rs10948059 and short tandem repeat polymorphism 1 were associated with prostate cancer risk. PMID: 24800880
    3. GNMT is an androgen receptor-targeted gene with its functional androgen response element located at +19/+33 of the first exon. PMID: 23883094
    4. The studies identify GNMT as a direct transcriptional target of the AR. PMID: 23997240
    5. DNA hypermethylation plays an important role in the repression of GNMT in hepatocellular carcinoma (HCC), and loss of GNMT in human HCC could promote the establishment of aberrant DNA methylation patterns at specific gene promoters PMID: 23475283
    6. single-nucleotide polymorphisms in VEGF, IL-10 and GNMT genes might have a synergistic role in the development of Prostate cancer PMID: 22850906
    7. Report SNPs that are highly associated with hepatic GNMT protein expression and the coordinate regulation of MAT1A levels. PMID: 22807109
    8. GNMT plays important role in maintaining normal function of brain; lack of GNMT causes dysfunction of important signaling pathways and results in alterations of neurotransmitter secretion as well as changes in neuropsychological behavior. PMID: 22264868
    9. GNMT is a crucial regulator in cholesterol metabolism and in inflammation, and contributes to the pathogenesis of atherosclerosis. PMID: 22415010
    10. In conclusion, GNMT regulates hepatocellular carcinoma growth in part through interacting with DEPDC6/DEPTOR and modulating mTOR/raptor signaling pathway PMID: 22160218
    11. GNMT may represent a novel marker of malignant progression and poor prognosis in prostate cancer PMID: 21572396
    12. This study suggests that the glycine N-methyltransferase STRP1 phenotype could modulate urinary 1-hydroxypyrene and 8-hydroxy-2'-deoxyguanosine levels in coke-oven workers exposed to PAHs. PMID: 21691217
    13. investigation of role of GNMT in methyl group homeostasis in liver: GNMT affects transmethylation kinetics, SAM synthesis, and conservation of methyl groups by limiting homocysteine remethylation flux; possible role of GNMT inactivation in hepatoma PMID: 21411609
    14. compound heterzygotes in the gene that encodes GNMT have evidence of mild liver disease PMID: 11810299
    15. Results suggest that GNMT alteration may be an early event in hepatocellular carcinoma development and that GNMT could be a new tumor susceptibility gene for hepatocellular carcinoma. PMID: 12566309
    16. GNMT 1289 C-->T polymorphism influences plasma homocysteine and is responsive to folate intake. PMID: 16317120
    17. GNMT is destabilized by a mutation. PMID: 17660255
    18. Glycine N-methyltransferase is a favorable prognostic marker for human cholangiocarcinoma. PMID: 18624901
    19. GNMT is a tumor suppressor gene for liver cancer, and it is associated with gender disparity in liver cancer susceptibility as shown in humans and in mouse models PMID: 19035462
    20. A transgenic mouse model is used together with in vitro methods to study the ability of the GNMT transgene to block the cytotoxic or carcinogenic activity of aflatoxin B1, a known causal agent of human hepatocellular carcinoma. PMID: 19146867
    21. Study identified a GNMT transcriptional start site at the 14th position upstream of the ATG codon; results indicate binding of the nuclear factor-Y (NF-Y) transcription factor to the CCAAT box (-71/-67) of the GNMT gene. PMID: 19439180

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  • 相关疾病：
    Glycine N-methyltransferase deficiency (GNMT deficiency)
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, Glycine N-methyltransferase family
  • 组织特异性：
    Abundant in liver.
  • 数据库链接：

    HGNC: 4415

    OMIM: 606628

    KEGG: hsa:27232

    STRING: 9606.ENSP00000361894

    UniGene: Hs.144914