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  4. Recombinant Human Glutaminyl-peptide cyclotransferase (QPCT)

Recombinant Human Glutaminyl-peptide cyclotransferase (QPCT)

In Stock
  • 货号：
    CSB-EP019135HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP019135HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) QPCT.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP019135HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) QPCT.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    QPCT
  • Uniprot No.：
  • 别名：
    5730422A13Rik; EC; GCT; Glutaminyl cyclase; Glutaminyl peptide cyclotransferase; Glutaminyl tRNA cyclotransferase; Glutaminyl-peptide cyclotransferase; Glutaminyl-tRNA cyclotransferase; Glutamyl cyclase; QC; qpct; QPCT_HUMAN; sQC
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    53.9kDa
  • 表达区域：
    29-361aa
  • 氨基酸序列
    VSPSASAWPEEKNYHQPAILNSSALRQIAEGTSISEMWQNDLQPLLIERYPGSPGSYAARQHIMQRIQRLQADWVLEIDTFLSQTPYGYRSFSNIISTLNPTAKRHLVLACHYDSKYFSHWNNRVFVGATDSAVPCAMMLELARALDKKLLSLKTVSDSKPDLSLQLIFFDGEEAFLHWSPQDSLYGSRHLAAKMASTPHPPGARGTSQLHGMDLLVLLDLIGAPNPTFPNFFPNSARWFERLQAIEHELHELGLLKDHSLEGRYFQNYSYGGVIQDDHIPFLRRGVPVLHLIPSPFPEVWHTMDDNEENLDESTIDNLNKILQVFVLEYLHL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    Full-length of mature human glutaminyl-peptide acyltransferase(QPCT) cDNA carrying an N-terminal 6xHis-SUMO-tag was expressed in E.coli. The resulting protein is the recombinant fusion protein consisting of 29-361aa of human QPCT. It was validated by LC-MS/MS analysis. The SDS-PAGE showed an about 47-51 kDa molecular mass of this protein and determined the purity of over 90%. In-stock QPCT proteins are offered now. This recombinant QPCT protein may be used to produce specific antibodies or in the studies of neuroscience.

    QPCT catalyzes the conversion of the N-terminal glutamate of proteins to pyroglutamate. This process makes the protein more resistant to protease degradation, more susceptible to hydrophobic interactions, aggregation, and neurotoxic. Ziyan Wang etc. demonstrated that QPCT knockdown led to the down-regulation of prophenoloxidase and crustin antimicrobial peptide. And QPCT may contribute to the replication of the White spot syndrome virus (WSSV).

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. Also catalyzes N-terminal pyroglutamate formation. In vitro, catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-amyloid-beta. May be involved in the N-terminal pyroglutamate formation of several amyloid-related plaque-forming peptides.
  • 基因功能参考文献：
    1. The addition of QC activity to core diagnostic CSF biomarkers may be of specific interest in clinical cases with discordant imaging and biochemical biomarker results. PMID: 28587659
    2. these findings demonstrate a significant association between common single nucleotide polymorphism within QPCT gene and schizophrenia risk in a Han Chinese population. PMID: 26572640
    3. the genetic risk of QPCT gene for schizophrenia also exists in the Han Chinese population. PMID: 26492838
    4. observations provide evidence for an involvement of QC in Alzheimer's disease pathogenesis and cognitive decline by QC-catalyzed pGlu-Abeta formation PMID: 24164736
    5. this study demonistrated that QPCT mRNAand protein from mononuclear cells correlated with theseverity of dementia. PMID: 23207485
    6. Upregulation of QPCT expression is associated with thyroid carcinomas. PMID: 23183267
    7. This study presents a first comparison of two mammalian QCs (human and mouse) containing typical, conserved post-translational modifications. PMID: 21671571
    8. The resilts of this studt provided histopathological evidence for QC being a prerequisite for pE-Abeta pathology in vivo and further underline the therapeutic potential of QC inhibition in Alzheimer Disease. PMID: 21301857
    9. Upon binding to PBD150, a large loop movement in gQC allows the inhibitor to be tightly held in its active site primarily by hydrophobic interactions. PMID: 21288892
    10. This study demonstrated that glutaminyl cyclase expression and pE-Abeta formation in subcortical brain regions( Edinger-Westphal nucleus, locus coeruleus and nucleus basalis Meynert) affected in Alzheimer's disease. PMID: 20383514
    11. Human isoQC proteins displayed a broad substrate specificity and preference for hydrophobic substrates, similar to the related QC. PMID: 19804409
    12. Functional expression of human glutaminyl cyclase in Pichia pastoris reveals the presence of a disulfide bond with an important role in the stabilization of active protein structure. PMID: 12196024
    13. human glutaminyl cyclase is a metal-dependent transferase PMID: 14522962
    14. Glutaminyl cyclase was expressed as a fully active 37 kDa enzyme with kcat/Km values of 14.3, 9.3, and 2.4 mM(-1)s(-1) for the substrates Gln-Gln, Gln-NH(2), and Gln-t-butyl ester, respectively PMID: 14680951
    15. Genetic variations in QPCT are important factors affecting the bone mineral density of adult women that contribute to susceptibility for osteoporosis. PMID: 15231017
    16. structure of human QC in free form and bound to a substrate and three imidazole-derived inhibitors PMID: 16135565
    17. Our results suggest that QPCT may be the QTL gene at chromosome 2p for spine BMD variation in the Chinese population PMID: 17687619
    18. combined steady-state enzyme kinetic and X-ray structural analyses of 11 single-mutation human QCs to investigate the roles of the H-bond network in catalysis PMID: 18072935
    19. The computations outline the important role of Trp329 in helping the substrate binding process and stabilizing the cyclization transition state. PMID: 18470930
    20. Golgi apparatus retention implies a "housekeeping" protein maturation machinery conducting glycosylation and pyroglutamyl formation. For these isoenzymes, apparently similar strategies evolved to retain the proteins in the Golgi complex. PMID: 18486145

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Glutaminyl-peptide cyclotransferase family
  • 数据库链接：

    HGNC: 9753

    OMIM: 607065

    KEGG: hsa:25797

    STRING: 9606.ENSP00000344829

    UniGene: Hs.79033