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  4. Recombinant Human GDH/6PGL endoplasmic bifunctional protein (H6PD)

Recombinant Human GDH/6PGL endoplasmic bifunctional protein (H6PD)

  • 货号：
    CSB-YP010111HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010111HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010111HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010111HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010111HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    H6PD
  • Uniprot No.：
  • 别名：
    6 phosphogluconolactonase; 6-phosphogluconolactonase; 6PGL; CORTRD1; G6PD; H form; G6PDH; G6PE_HUMAN; GDH; GDH/6PGL endoplasmic bifunctional protein; Glucose 1 dehydrogenase; Glucose 6 phosphate dehydrogenase; salivary; Glucose dehydrogenase; Glucose dehyrogenase; Gpd1; H6pd; Hexose 6 phosphate dehydrogenase (glucose 1 dehydrogenase); Hexose 6 phosphate dehydrogenase; Hexose-6-phosphate dehydrogenase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    20-791
  • 氨基酸序列
    Q ELQGHVSIIL LGATGDLAKK YLWQGLFQLY LDEAGRGHSF SFHGAALTAP KQGQELMAKA LESLSCPKDM APSHCAEHKD QFLQLSQYRQ LKTAEDYQAL NKDIEAQLQH AGLREAGRIF YFSVPPFAYE DIARNINSSC RPGPGAWLRV VLEKPFGHDH FSAQQLATEL GTFFQEEEMY RVDHYLGKQA VAQILPFRDQ NRKALDGLWN RHHVERVEII MKETVDAEGR TSFYEEYGVI RDVLQNHLTE VLTLVAMELP HNVSSAEAVL RHKLQVFQAL RGLQRGSAVV GQYQSYSEQV RRELQKPDSF HSLTPTFAAV LVHIDNLRWE GVPFILMSGK ALDERVGYAR ILFKNQACCV QSEKHWAAAQ SQCLPRQLVF HIGHGDLGSP AVLVSRNLFR PSLPSSWKEM EGPPGLRLFG SPLSDYYAYS PVRERDAHSV LLSHIFHGRK NFFITTENLL ASWNFWTPLL ESLAHKAPRL YPGGAENGRL LDFEFSSGRL FFSQQQPEQL VPGPGPAPMP SDFQVLRAKY RESPLVSAWS EELISKLAND IEATAVRAVR RFGQFHLALS GGSSPVALFQ QLATAHYGFP WAHTHLWLVD ERCVPLSDPE SNFQGLQAHL LQHVRIPYYN IHPMPVHLQQ RLCAEEDQGA QIYAREISAL VANSSFDLVL LGMGADGHTA SLFPQSPTGL DGEQLVVLTT SPSQPHRRMS LSLPLINRAK KVAVLVMGRM KREITTLVSR VGHEPKKWPI SGVLPHSGQL VWYMDYDAFL G
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Bifunctional enzyme localized in the lumen of the endoplasmic reticulum that catalyzes the first two steps of the oxidative branch of the pentose phosphate pathway/shunt, an alternative to glycolysis and a major source of reducing power and metabolic intermediates for biosynthetic processes. Has a hexose-6-phosphate dehydrogenase activity, with broad substrate specificity compared to glucose-6-phosphate 1-dehydrogenase/G6PD, and catalyzes the first step of the pentose phosphate pathway. In addition, acts as a 6-phosphogluconolactonase and catalyzes the second step of the pentose phosphate pathway. May have a dehydrogenase activity for alternative substrates including glucosamine 6-phosphate and glucose 6-sulfate. The main function of this enzyme is to provide reducing equivalents such as NADPH to maintain the adequate levels of reductive cofactors in the oxidizing environment of the endoplasmic reticulum. By producing NADPH that is needed by reductases of the lumen of the endoplasmic reticulum like corticosteroid 11-beta-dehydrogenase isozyme 1/HSD11B1, indirectly regulates their activity.
  • 基因功能参考文献：
    1. Data indicate the tetramer as the most active form of glucose-6-phosphate dehydrogenase (G6PDH). PMID: 28370139
    2. cortisol reduces glucose-6-phosphate (G6P) flux through H6PDH by increasing luminal NADPH, thereby allowing more G6P for hydrolysis via G6Pase PMID: 26860459
    3. Inhibition of glucose-6-phosphate dehydrogenase sensitizes cisplatin-resistant cells to death. PMID: 26337086
    4. The G allele of rs6688832 in H6PD might exert potential genetic protective role against the development of PCOS, especially in overweight women. PCOS patients with AG genotype of rs6688832 might confer risk to the phenotype of hyperandrogenemia of PCOS. PMID: 26452272
    5. The aim of the work was to investigate the expression of HSD11B1, HSD11B2, H6PDH, and glucocorticoids receptor (GR) mRNA in subcutaneous adipose tissue (SAT) from obese women with or without polycystic ovary syndrome. PMID: 23979790
    6. Cytoplasmic Mg2+ regulates glucose 6-phosphate utilization by reticular H6PD. PMID: 24631573
    7. GR and p300 are involved in the induction of H6PD by cortisol in human amnion fibroblasts PMID: 23125313
    8. Polymorphisms in the H6PD gene may not be associated with type 2 diabetes and the metabolic syndrome. PMID: 21869537
    9. Antioxidant supplementation was noted to increase G6PDH in the pentose phosphate cycle and 18S rRNA in the ribosome. There were no significant changes in the gene expression levels of beta-ACT PMID: 22285204
    10. R453Q and D151A variants of the H6PD gene are associated with PCOS and obesity, respectively, and may contribute to the PCOS phenotype by influencing obesity, insulin resistance and hyperandrogenism. PMID: 22306327
    11. Results suggest a novel role for the H6PD gene in atherosclerosis susceptibility. PMID: 21858044
    12. the H6PD gene is very polymorphic and missense variants are common, coding variants rarely (<1.5%) are responsible for hyperandrogenemic polycystic ovary syndrome. PMID: 21050867
    13. identified a mitochondrial matrix-associated G6PDH and also provide evidence that metabolic state/glucose availability modulate enzymatic sources of NADPH PMID: 20228249
    14. This study corroborates the association of one locus determined by genome-wide association study and points to H6PD as a new candidate gene for multiple sclerosis. PMID: 19935835
    15. findings indicate that the decrease in G6PDH activity that occurs after exposure of lung epithelial cells to chrysotile results from the carbonylation of G6PDH by TBARS PMID: 20211231
    16. Mutations in the genes encoding 11beta-hydroxysteroid dehydrogenase type 1 and hexose-6-phosphate dehydrogenase interact to cause cortisone reductase deficiency. PMID: 12858176
    17. H6PDH directly determines the reaction direction of 11beta-Hydroxysteroid dehydrogenase1 in intact cells as an oxoreductase. PMID: 15280030
    18. A new chemiluminescent method was applied to the determination of G6PDH in healthy and enzyme-deficient individuals. PMID: 15767211
    19. Two new missense mutations in glucose-6-phosphate dehydrogenase gene associated with chronic hemolytic anemia. PMID: 16079115
    20. Mutations found in beta thassemia patients complicated by liver disease. PMID: 16079116
    21. The observed constant expression of H6PD suggests that HSD11B1 acts as a reductase throughout the adipogenesis process in human ADMSCs and murine 3T3-L1 cells. PMID: 18586838
    22. Hepatic 11beta-HSD1 and H6PDH are closely interlinked. 11beta-HSD1 gene expression does not seem to be involved in the pathogenesis of fatty liver or nonalcoholic steatohepatitis. PMID: 18665910
    23. Data show that adipose tissue 11-beta-Hydroxysteroid Dehydrogenase Type 1 and Hexose-6-Phosphate Dehydrogenase gene expressions are increased in patients with type 2 diabetes mellitus. PMID: 18963204
    24. The impact of H6PDH on the modulation of 11beta-HSD1-dependent interconversion of cortisone and cortisol by inhibitors and alternative substrates, was investigated. PMID: 19010388
    25. Using co-immunoprecipitation experiments with purified H6PDH and 11beta-HSD1, and with cell lysates expressing H6PDH and 11beta-HSD1, we observe direct physical interaction between the two enzymes. PMID: 19121282

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  • 相关疾病：
    Cortisone reductase deficiency 1 (CORTRD1)
  • 亚细胞定位：
    Endoplasmic reticulum lumen.
  • 蛋白家族：
    Glucose-6-phosphate dehydrogenase family; Glucosamine/galactosamine-6-phosphate isomerase family, 6-phosphogluconolactonase subfamily
  • 组织特异性：
    Present in most tissues examined, strongest in liver.
  • 数据库链接：

    HGNC: 4795

    OMIM: 138090

    KEGG: hsa:9563

    STRING: 9606.ENSP00000366620

    UniGene: Hs.463511