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  4. Recombinant Human G-protein-signaling modulator 1 (GPSM1)

Recombinant Human G-protein-signaling modulator 1 (GPSM1)

  • 货号：
    CSB-YP801266HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP801266HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP801266HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP801266HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP801266HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GPSM1
  • Uniprot No.：
  • 别名：
    Activator of G-protein signaling 3; AGS3; AGS3 homolog ; C10a; G protein signaling modulator 1 (AGS3 like; C. elegans) ; G-protein-signaling modulator 1; GPSM1; GPSM1_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-675
  • 氨基酸序列
    MAGPAPPVAD ELPGPAARRL YSRMEASCLE LALEGERLCK AGDFKTGVAF FEAAVQVGTE DLKTLSAIYS QLGNAYFYLK EHGRALEYHK HDLLLARTIG DRMGEAKASG NLGNTLKVLG RFDEAAVCCQ RHLSIAQEQG DKVGEARALY NIGNVYHAKG KQLSWNAANA TQDPGHLPPD VRETLCKASE FYERNLSLVK ELGDRAAQGR AYGNLGNTHY LLGNFTEATT FHKERLAIAK EFGDKAAERR AYSNLGNAHV FLGRFDVAAE YYKKTLQLSR QLRDQAVEAQ ACYSLGNTYT LLQDYERAAE YHLRHLLIAQ ELADRVGEGR ACWSLGNAYV SMGRPAQALT FAKKHLQISQ EIGDRHGELT ARMNVAQLQL VLGRLTSPAA SEKPDLAGYE AQGARPKRTQ RLSAETWDLL RLPLEREQNG DSHHSGDWRG PSRDSLPLPV RSRKYQEGPD AERRPREGSH SPLDSADVRV HVPRTSIPRA PSSDEECFFD LLTKFQSSRM DDQRCPLDDG QAGAAEATAA PTLEDRIAQP SMTASPQTEE FFDLIASSQS RRLDDQRASV GSLPGLRITH SNAGHLRGHG EPQEPGDDFF NMLIKYQSSR IDDQRCPPPD VLPRGPTMPD EDFFSLIQRV QAKRMDEQRV DLAGGPEQGA GGPPEPQQQC QPGAS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G-protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction.
  • 基因功能参考文献：
    1. The G-protein Regulatory motif of AGS3 is critical for regulating MUC1/Muc1 expression and cytokine production in the inflammatory microenvironment. PMID: 27270970
    2. Data support the notion that the Galpha, but not Gbetagamma, arm of the Gi/o signalling is involved in TRPC4 activation and unveil new roles for RGS and AGS3 in fine-tuning TRPC4 activities. PMID: 26987813
    3. Activator of G-protein Signaling 3 is an important regulator of esophageal squamous cell carcinoma proliferation PMID: 25812748
    4. Results confirmed that Ric-8A can directly bind to AGS3S but failed to facilitate Galpha(i)-induced suppression of adenylyl cyclase, suggesting that it may not serve as a guanine exchange factor for AGS3/Galpha(i/o)-GDP complex in a cellular environment. PMID: 25480567
    5. High GPSM1 gene methylation is associated with gastric tumor aggressiveness. PMID: 25740824
    6. Data identified three new loci for type 2 diabetes with genome-wide significance: MIR129-LEP, GPSM1 and SLC16A13. PMID: 23945395
    7. Our data indicate that decreased expression of AGS3 is correlated with reduced levels of p-CREB in the apoptotic model. The negative role of AGS3 in cell apoptosis was further confirmed by knocking down AGS3. PMID: 24307516
    8. these results indicate that GRK6 complexes with AGS3-Galphai2 to regulate CXCR2-mediated leukocyte functions at different levels, including downstream effector activation, receptor trafficking, and expression at the cell membrane. PMID: 24510965
    9. These results provide mechanistic insights into how reversible modulation of Galpha(i3) activity by AGS3 and GIV maintains the delicate equilibrium between promotion and inhibition of autophagy. PMID: 21209316
    10. AGS3 receptor coupling to both Galphabetagamma and GPR-Galpha(i) offer additional flexibility for systems to respond and adapt to challenges and orchestrate complex behaviors PMID: 20716524
    11. unlike wild-type AGS3, over-expression of an AGS3 mutant lacking this modification fails to enhance macroautophagic activity. These observations imply that AGS3 phosphorylation may participate in the modulation of macroautophagy PMID: 20126274
    12. These data present AGS3, G-proteins, and mInsc as candidate proteins involved in regulating cellular stress associated with protein-processing pathologies. PMID: 20065032
    13. AGS3 is involved in an early event during the autophagic pathway probably prior to the formation of the autophagosome. PMID: 12642577
    14. cortical localization in mitotic cell culture systems and requirement for normal cell cycle progression PMID: 12925752
    15. data support a model wherein AGS3 modulates the protein trafficking along the TGN/plasma membrane/endosome loop PMID: 17991770
    16. The GPR motif in this protein and other proteins is actually associated with activity as a GDI - guanine nucleotide dissociation inhibitor and not GTPase activator as initially suggested. PMID: 10969064

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  • 亚细胞定位：
    Cytoplasm, cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    GPSM family
  • 组织特异性：
    Expressed in intestinal cells.
  • 数据库链接：

    HGNC: 17858

    OMIM: 609491

    KEGG: hsa:26086

    STRING: 9606.ENSP00000392828

    UniGene: Hs.239370