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  4. Recombinant Human Fructose-bisphosphate aldolase A (ALDOA)

Recombinant Human Fructose-bisphosphate aldolase A (ALDOA)

  • 货号：
    CSB-EP001583HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP000154h could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ALDOA.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP000154h could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ALDOA.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    ALDA; Aldo1; ALDOA; ALDOA_HUMAN; Aldolase 1; Aldolase A; Aldolase A fructose bisphosphatase; Aldolase A fructose bisphosphate; Aldolase; fructose-bisphosphate A; Epididymis secretory sperm binding protein Li 87p; FRUCTOALDOLASE A; Fructose 1 6 bisphosphate triosephosphate lyase; Fructose bisphosphate aldolase A; Fructose bisphosphate aldolase; FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE A; Fructose-bisphosphate aldolase A; Fructose-bisphosphate aldolase A Muscle-type; GSD12; HEL S 87p; Lung cancer antigen NY LU 1; Lung cancer antigen NY-LU-1; MGC10942; MGC17716; MGC17767; Muscle type aldolase; Muscle-type aldolase; RNALDOG5
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    66.3kDa
  • 表达区域：
    2-364aa
  • 氨基酸序列
    PYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFVSNHAY
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
  • 基因功能参考文献：
    1. Results showed that ALDOA was upregulated in renal cell carcinoma (RCC) samples and cell lines and significantly associated with metastasis and survival. Overall, data revealed that ALDOA functions as a tumor promoter, plays a prominent role in proliferation, migration, and invasion of RCC cells with high expression, and may promote EMT and activate the Wnt/betacatenin signaling pathway. PMID: 29693182
    2. The results indicate that ALDOA and PGK1 may indicate resistance to cisplatin in osteosarcoma PMID: 29199648
    3. Knockdown of ALDOA in QBC939 and RBE cells attenuated the cell proliferation and induced a higher apoptosis rate. PMID: 30121648
    4. Silencing aldolase A suppressed colon cancer cell proliferation and invasion and inhibited the epithelial-mesenchymal transition phenotype. Aldolase A protein expression in colon cancer was related to tumor location, tumor clinical stage and survival. PMID: 29453983
    5. mir-122 and its targets G6PC3, ALDOA and CS play roles in the hypoxia responses that regulate glucose and energy metabolism and can serve as hypoxia biomarkers. PMID: 27793029
    6. Aldolase A promotes lung cancer metastasis via PHD-mediated stabilization of HIF-1alpha and the subsequent activation of MMP9. PMID: 28610954
    7. Findings show that ALDOA expression is up-regulated in colorectal cancer (CRC) and is a hypoxia-inducible prognostic factor that is closely related to CRC malignancy. PMID: 28000858
    8. Overexpression of ALDOA is associated with colorectal cancer. PMID: 27468721
    9. In vitro and in vivo results demonstrated that ALDOA was associated with proliferation and metastasis of pancreatic cancer cells. PMID: 26854714
    10. Study shows that PI3K directly coordinates glycolysis with cytoskeletal dynamics in an AKT-independent manner. Growth factors or insulin stimulate the PI3K-dependent activation of Rac, leading to disruption of the actin cytoskeleton, release of filamentous actin-bound aldolase A, and an increase in aldolase activity. PMID: 26824656
    11. Our results expand the clinical spectrum of aldolase A deficiency to isolated temperature-dependent rhabdomyolysis, and suggest that thermolability may be tissue specific. We also propose a treatment for this severe disease PMID: 25392908
    12. Study provides evidence supporting a critical functional role of ALDOA in osteosarcoma progression, metastasis and perhaps chemoresistance. PMID: 25215901
    13. ALDOC, Aldolase A (ALDOA) and Aldolase B (ALDOB) activate Wnt signaling. PMID: 24993527
    14. ALDOA is highly expressed in lung squamous cell carcinoma (LSCC) and its expression level is correlated with LSCC metastasis, grades, differentiation status and poor prognosis. PMID: 24465716
    15. The expression of ALDOA and/or SULT1A3 is significantly higher. PMID: 22949271
    16. The results presented here point to ALDA as a factor involved in the regulation of cells proliferation. PMID: 23886627
    17. The new prognostic biomarkers GRP78, Fructose-bisphosphate Aldolase A (ALDOA), Carbonic Anhydrase I (CA1) and Peptidyl-prolyl cis-trans isomerase A or Cyclophilin A (PPIA)) provided good survival prediction for TNM stage I-IV patients. PMID: 22996014
    18. ALDOA is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
    19. Data that aldolase forms a complex with ARNO/Arf6 and the V-ATPase and that it may contribute to remodeling of the actin cytoskeleton. PMID: 21307348
    20. Posttranslational nitration of aldolase A may be an important pathway that regulates mast cell phenotype and function. PMID: 20511553
    21. ALDOA is involved in keratinocyte migration following the induction of lamellipodia formation, and ALDOA-related migration is enhanced by EGF PMID: 20362419
    22. melanoma antigen expressed in G361, a representative melanoma cell line/ reacted with autoantibodies in patient sera PMID: 20181627
    23. Gly346 is crucial for the correct conformation and function of aldolase A, because it governs the entry/release of the substrates into/from the enzyme cleft, and/or allows important C-terminal residues to approach the active site. PMID: 14766013
    24. The existence of highly AMP-sensitive muscle-like FBPase, activity of which is regulated by metabolite-dependent interaction with aldolase enables the precise regulation of muscle energy expenditures. PMID: 18214967
    25. Results identify VDAC2 and aldolase A as membrane proteins of K562 cells with increased expression under iron deprivation. PMID: 18278581
    26. Aldolase A may play a role in the radio-response of human cells, probably in nuclei, in addition to its glycolytic role in the cytosol. PMID: 18328256
    27. ZNF224 recruits the arginine methyltransferase PRMT5 on the transcriptional repressor complex of the aldolase A gene PMID: 19741270

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  • 相关疾病：
    Glycogen storage disease 12 (GSD12)
  • 亚细胞定位：
    Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, M line.
  • 蛋白家族：
    Class I fructose-bisphosphate aldolase family
  • 数据库链接：

    HGNC: 414

    OMIM: 103850

    KEGG: hsa:226

    STRING: 9606.ENSP00000336927

    UniGene: Hs.513490