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  4. Recombinant Human Forkhead box protein K1 (FOXK1)

Recombinant Human Forkhead box protein K1 (FOXK1)

  • 货号：
    CSB-YP008824HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP008824HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP008824HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP008824HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP008824HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    FOXK1
  • Uniprot No.：
  • 别名：
    A630048H08Rik; AI463295; ENSMUSG00000075577; FHX; Fork head; Forkhead box protein K1; FOXJ2 forkhead factor; FOXK1; FOXK1_HUMAN; Gm10868; MNF; Myocyte nuclear factor
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-733
  • 氨基酸序列
    AEVGEDSGA RALLALRSAP CSPVLCAAAA AAAFPAAAPP PAPAQPQPPP GPPPPPPPPL PPGAIAGAGS SGGSSGVSGD SAVAGAAPAL VAAAAASVRQ SPGPALARLE GREFEFLMRQ PSVTIGRNSS QGSVDLSMGL SSFISRRHLQ LSFQEPHFYL RCLGKNGVFV DGAFQRRGAP ALQLPKQCTF RFPSTAIKIQ FTSLYHKEEA PASPLRPLYP QISPLKIHIP EPDLRSMVSP VPSPTGTISV PNSCPASPRG AGSSSYRFVQ NVTSDLQLAA EFAAKAASEQ QADTSGGDSP KDESKPPFSY AQLIVQAISS AQDRQLTLSG IYAHITKHYP YYRTADKGWQ NSIRHNLSLN RYFIKVPRSQ EEPGKGSFWR IDPASEAKLV EQAFRKRRQR GVSCFRTPFG PLSSRSAPAS PTHPGLMSPR SGGLQTPECL SREGSPIPHD PEFGSKLASV PEYRYSQSAP GSPVSAQPVI MAVPPRPSSL VAKPVAYMPA SIVTSQQPAG HAIHVVQQAP TVTMVRVVTT SANSANGYIL TSQGAAGGSH DAAGAAVLDL GSEARGLEEK PTIAFATIPA AGGVIQTVAS QMAPGVPGHT VTILQPATPV TLGQHHLPVR AVTQNGKHAV PTNSLAGNAY ALTSPLQLLA TQASSSAPVV VTRVCEVGPK EPAAAVAATA TTTPATATTA SASASSTGEP EVKRSRVEEP SGAVTTPAGV IAAAGPQGPG TGE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis, muscle cell differentiation and autophagy. Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context. Together with FOXK2, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen. Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4. Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate. Involved in mTORC1-mediated metabolic reprogramming: in response to mTORC1 signaling, translocates into the nucleus and regulates the expression of genes associated with glycolysis and downstream anabolic pathways, such as HIF1A, thereby regulating glucose metabolism. Together with FOXK2, acts as a negative regulator of autophagy in skeletal muscle: in response to starvation, enters the nucleus, binds the promoters of autophagy genes and represses their expression, preventing proteolysis of skeletal muscle proteins. Acts as a transcriptional regulator of the myogenic progenitor cell population in skeletal muscle. Binds to the upstream enhancer region (CCAC box) of myoglobin (MB) gene, regulating the myogenic progenitor cell population. Promotes muscle progenitor cell proliferation by repressing the transcriptional activity of FOXO4, thereby inhibiting myogenic differentiation. Involved in remodeling processes of adult muscles that occur in response to physiological stimuli. Required to correct temporal orchestration of molecular and cellular events necessary for muscle repair. Represses myogenic differentiation by inhibiting MEFC activity. Positively regulates Wnt/beta-catenin signaling by translocating DVL into the nucleus. Reduces virus replication, probably by binding the interferon stimulated response element (ISRE) to promote antiviral gene expression.
  • 基因功能参考文献：
    1. Knockdown of FOXK1 reduced cell viability and HK2 expression, decreased glucose consumption and lactate production in liver cancer cells. Furthermore, FOXK1 knockdown suppressed the activation of Akt/mTOR pathway. Inhibition of Akt/mTOR pathway reduced cell viability and glycolysis of liver cancer cells. PMID: 30312701
    2. s show that a nuclear-cytoplasmic transport system is necessary for the mTORC1-FOXK1 signal transduction. This reaction is mediated by a shuttling protein B56, which is a regulatory subunit of PP2A and plays an essential role in the mTORC1-dependent dephosphorylation of FOXK1. PMID: 29845697
    3. Results demonstrated that knockdown of FOXK1 inhibited the proliferation and metastasis of prostate cancer, at least in part, through suppressing the Wnt/beta-catenin signaling pathway. PMID: 28267429
    4. demonstrated that circMAN2B2 acts as an oncogenic role in lung cancer through promoting FOXK1 expression by sponging miR-1275 PMID: 29550475
    5. Knockdown of FOXK1 significantly inhibited hepatocellular carcinoma cell proliferation, migration and invasion. PMID: 28551547
    6. For FOXK1 and KCNA7, the age effect on the sperm epigenome was replicated in an independent cohort of 188 sperm samples. PMID: 28171595
    7. The higher expression of FOXK1 could indicate a poor prognosis in colorectal carcinoma (CRC) patients since FOXK1 induces epithelial-mesenchymal transition and promotes CRC cell invasion in vitro and in vivo. PMID: 27223064
    8. FOXK1 was overexpressed in esophageal cancer (EC) tissues compared with corresponding non-tumor tissues using immunohistochemistry. And high FOXK1 expression was related to poor differentiation of EC. The Kaplan-Meier curve indicated that high FOXK1 expression may result in poor prognosis of EC patients. PMID: 29050933
    9. c-jun promoted FOXK1-mediated proliferation and metastasis via orthotopic implantation. PMID: 27882939
    10. These findings implicate FOXK1 as a cell cycle and growth modulator that inhibits apoptosis in colon cancer cells. FOXK1-shRNA may serve as a novel and potent therapeutic agent, alone or with 5-FU, against colon cancer PMID: 27571921
    11. Data indicated the function of Foxk1 as a tumor suppressor in facilitating angiogenesis and metastasis in breast cancer. PMID: 26732382
    12. FOXK1 protein levels and activity are regulated by associating with JLP and PLK1 PMID: 26468278
    13. Overall, these results demonstrate a novel role for FOXK1 in regulating the expression of antiviral genes via Nup98, from insects to humans. PMID: 25852164
    14. FOXK1 and FOXK2 positively regulate Wnt/beta-catenin signaling by translocating dishevelled proteins into the nucleus. PMID: 25805136
    15. FOXK1 binds to the promoter and regulates expression of DHFR, TYMS, GSDMD, and the E2F binding partner TFDP1. PMID: 22740631
    16. crystallographic analysis of the FOXK1a-DNA complex PMID: 16624804

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 组织特异性：
    Expressed both developing and adult tissues. In adults, significant expression is seen in tumors of the brain, colon and lymph node.
  • 数据库链接：

    HGNC: 23480

    OMIM: 616302

    KEGG: hsa:221937

    STRING: 9606.ENSP00000328720

    UniGene: Hs.487393